LOCUS       sequence-469311-        6291 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid for RSV A2 F.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6291)
  AUTHORS   Simonich CAL, McMahon TE, Ju X, Yu TC, Brunette N, Stevens-Ayers T, 
            Boeckh MJ, King NP, Greninger AL, Bloom JD
  TITLE     RSV F evolution escapes some monoclonal antibodies but does not 
            strongly erode neutralization by human polyclonal sera.
  JOURNAL   J Virol. 2025 Jul 3:e0053125. doi: 10.1128/jvi.00531-25.
  PUBMED    40607811
REFERENCE   2  (bases 1 to 6291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2025 Jul 3:e0053125. doi: 10.1128/jvi.00531-25."
COMMENT     Sequence Label: HDM_RSV_A2_F
FEATURES             Location/Qualifiers
     source          1..6291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        1253..1632
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1633..1836
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1787..1807
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1833..1857
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1950..2425
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     regulatory      2493..2502
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4232..4248)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4233..4249)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    4342..4736
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(5070..5088)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5156..5260
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5261..5329,5330..6121)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5479..5498)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cggagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag
      661 ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg
      721 tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc
      781 accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa
      841 cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccaatgat
      901 cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc
      961 tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag
     1021 aatcataatg gggaaggcca tccagcctcg cgtcggggag ctttttgcaa aagcctaggc
     1081 ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct
     1141 ctgcataaat aaaaaaaatt agtcagccat gagcttggcc cattgcatac gttgtatcca
     1201 tatcataata tgtacattta tattggctca tgtccaacat taccgccatg ttgacattga
     1261 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     1321 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     1381 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     1441 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     1501 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     1561 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     1621 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     1681 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     1741 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     1801 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     1861 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
     1921 ccctcgaagc tgatcctgag aacttcaggg tgagtctatg ggacccttga tgttttcttt
     1981 ccccttcttt tctatggtta agttcatgtc ataggaaggg gagaagtaac agggtacaca
     2041 tattgaccaa atcagggtaa ttttgcattt gtaattttaa aaaatgcttt cttcttttaa
     2101 tatacttttt tgtttatctt atttctaata ctttccctaa tctctttctt tcagggcaat
     2161 aatgatacaa tgtatcatgc ctctttgcac cattctaaag aataacagtg ataatttctg
     2221 ggttaaggca atagcaatat ttctgcatat aaatatttct gcatataaat tgtaactgat
     2281 gtaagaggtt tcatattgct aatagcagct acaatccagc taccattctg cttttatttt
     2341 atggttggga taaggctgga ttattctgag tccaagctag gcccttttgc taatcatgtt
     2401 catacctctt atcttcctcc cacagctcct gggcaacgtg ctggtctgtg tgctggccca
     2461 tcactttggc aaagaattcc gcgggcggcc gcgccaccat ggaattactg atcctgaagg
     2521 ccaatgccat caccacaatc ctgacagccg tgaccttctg cttcgccagc ggccagaata
     2581 tcaccgaaga gttctaccag agcacctgca gcgccgtgtc caagggctat ctgtctgccc
     2641 tgagaacagg ctggtatact tccgtgatca ccatcgagct gagcaacatc aaggaaaaca
     2701 agtgtaatgg cacggacgcc aaggtcaaac tgatcaagca ggagctggac aagtacaaga
     2761 acgccgtgac cgagctgcag ctgctgatgc agtctacgcc tgctacaaac aaccgcgcca
     2821 gaagagagct gcccagattc atgaactaca ccctgaacaa cgcaaagaag actaatgtga
     2881 cactgtctaa gaaaagaaaa cggcggtttc tgggctttct cctcggcgtg ggcagcgcta
     2941 tcgccagcgg cgtggccgtg agcaaagtcc tgcacctgga aggagaggtg aacaagatca
     3001 agtctgctct gctgagcacc aacaaggccg tggtgtccct gagcaacgga gtgagcgtgc
     3061 tgacaagcaa ggtgctggat ctgaagaact acatcgataa gcaactgctg cctatcgtta
     3121 ataagcagag ctgcagcatc agcaatatcg aaaccgtgat cgaattccag cagaaaaata
     3181 acaggctgct tgagatcaca cgggaatttt ctgtcaacgc tggcgtgaca acccctgtga
     3241 gcacatacat gctgacaaac agcgagctgc tgtctctgat caacgacatg cctatcacca
     3301 atgaccagaa aaagctgatg tctaacaacg tgcagatcgt gagacagcag agctacagca
     3361 tcatgagcat catcaaggaa gaagtgctcg cctacgtggt ccagctgccc ctgtacggcg
     3421 tgatcgacac cccttgctgg aagctgcaca cctcccctct gtgtaccacc aacacaaagg
     3481 aaggcagcaa tatctgtctg accagaaccg acagaggctg gtattgtgac aacgccggct
     3541 ctgtgtcctt cttcccccag gccgagacat gcaaggtgca atccaacaga gtgttctgcg
     3601 atacaatgaa ctccctgacc ctgccatctg aggtgaacct gtgcaacgtg gacatcttca
     3661 accccaagta cgactgcaag atcatgacca gcaagaccga tgtgtccagc agcgtgatca
     3721 ccagcctggg agccattgtg tcttgctacg gcaagaccaa gtgcacagct agcaacaaga
     3781 accggggcat catcaagacc tttagcaacg gttgtgatta cgtgagcaac aaaggagtag
     3841 acaccgtgag cgttggcaac actctgtact acgtgaacaa gcaagagggc aaaagcctgt
     3901 acgtgaaggg cgagcctatc atcaacttct acgaccctct ggtgttccct agcgatgagt
     3961 tcgacgcctc tatctcacag gtgaacgaga aaatcaacca gagcctggcc ttcatcagaa
     4021 agagcgacga gctgctgcac aacgtgaatg ccggaaagtc caccaccaac atcatgatta
     4081 ccaccattat catcgtgatc atcgtgatcc tcctgagcct gatcgccgtg ggcctgctgc
     4141 tttactgcaa ggcccggagc accccagtga cactgagcaa ggaccagctg tctggaatca
     4201 acaatatcgc tttcagcaac tgaaagctta tcgataccgt cgacctcgag ggcccagatc
     4261 taattcaccc caccagtgca ggctgcctat cagaaagtgg tggctggtgt ggctaatgcc
     4321 ctggcccaca agtatcacta agctcgcttt cttgctgtcc aatttctatt aaaggttcct
     4381 ttgttcccta agtccaacta ctaaactggg ggatattatg aagggccttg agcatctgga
     4441 ttctgcctaa taaaaaacat ttattttcat tgcaatgatg tatttaaatt atttctgaat
     4501 attttactaa aaagggaatg tgggaggtca gtgcatttaa aacataaaga aatgaagagc
     4561 tagttcaaac cttgggaaaa tacactatat cttaaactcc atgaaagaag gtgaggctgc
     4621 aaacagctaa tgcacattgg caacagcccc tgatgcctat gccttattca tccctcagaa
     4681 aaggattcaa gtagaggctt gatttggagg ttaaagtttt gctatgctgt attttacatt
     4741 acttattgtt ttagctgtcc tcatgaatgt cttttcacta cccatttgct tatcctgcat
     4801 ctctcagcct tgactccact cagttctctt gcttagagat accacctttc ccctgaagtg
     4861 ttccttccat gttttacggc gagatggttt ctcctcgcct ggccactcag ccttagttgt
     4921 ctctgttgtc ttatagaggt ctacttgaag aaggaaaaac agggggcatg gtttgactgt
     4981 cctgtgagcc cttcttccct gcctccccca ctcacagtga cccggaatcc ctcgacatgg
     5041 cagtctagat cattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     5101 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     5161 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     5221 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     5281 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     5341 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     5401 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     5461 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     5521 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     5581 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     5641 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     5701 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     5761 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     5821 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     5881 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     5941 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     6001 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     6061 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     6121 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     6181 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6241 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt c
//
