LOCUS       sequence-469314-        6294 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid for RSV A F from 2020.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6294)
  AUTHORS   Simonich CAL, McMahon TE, Ju X, Yu TC, Brunette N, Stevens-Ayers T, 
            Boeckh MJ, King NP, Greninger AL, Bloom JD
  TITLE     RSV F evolution escapes some monoclonal antibodies but does not 
            strongly erode neutralization by human polyclonal sera.
  JOURNAL   J Virol. 2025 Jul 3:e0053125. doi: 10.1128/jvi.00531-25.
  PUBMED    40607811
REFERENCE   2  (bases 1 to 6294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2025 Jul 3:e0053125. doi: 10.1128/jvi.00531-25."
COMMENT     Sequence Label: HDM_RSV_A_2020_F
FEATURES             Location/Qualifiers
     source          1..6294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        1253..1632
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1633..1836
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1787..1807
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1833..1857
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1950..2425
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     regulatory      2493..2502
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4235..4251)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4236..4252)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    4345..4739
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(5073..5091)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5159..5263
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5264..5332,5333..6124)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5482..5501)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cggagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag
      661 ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg
      721 tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc
      781 accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa
      841 cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccaatgat
      901 cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc
      961 tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag
     1021 aatcataatg gggaaggcca tccagcctcg cgtcggggag ctttttgcaa aagcctaggc
     1081 ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct
     1141 ctgcataaat aaaaaaaatt agtcagccat gagcttggcc cattgcatac gttgtatcca
     1201 tatcataata tgtacattta tattggctca tgtccaacat taccgccatg ttgacattga
     1261 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     1321 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     1381 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     1441 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     1501 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     1561 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     1621 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     1681 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     1741 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     1801 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     1861 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
     1921 ccctcgaagc tgatcctgag aacttcaggg tgagtctatg ggacccttga tgttttcttt
     1981 ccccttcttt tctatggtta agttcatgtc ataggaaggg gagaagtaac agggtacaca
     2041 tattgaccaa atcagggtaa ttttgcattt gtaattttaa aaaatgcttt cttcttttaa
     2101 tatacttttt tgtttatctt atttctaata ctttccctaa tctctttctt tcagggcaat
     2161 aatgatacaa tgtatcatgc ctctttgcac cattctaaag aataacagtg ataatttctg
     2221 ggttaaggca atagcaatat ttctgcatat aaatatttct gcatataaat tgtaactgat
     2281 gtaagaggtt tcatattgct aatagcagct acaatccagc taccattctg cttttatttt
     2341 atggttggga taaggctgga ttattctgag tccaagctag gcccttttgc taatcatgtt
     2401 catacctctt atcttcctcc cacagctcct gggcaacgtg ctggtctgtg tgctggccca
     2461 tcactttggc aaagaattcc gcgggcggcc gcgccaccat ggagctgccc atcctgaaaa
     2521 ccaacgccat cactacaatc ctggccgccg tgactctgtg cttcgcctca tcccagaaca
     2581 tcaccgagga gttttatcag tcaacctgta gtgccgtgtc taagggctat ctgtccgccc
     2641 tgcgcaccgg ttggtatacc tctgtgatca ctatcgaact gagcaatatt aaggagaaca
     2701 agtgcaacgg cacagacgcc aaggtgaagc tgatcaagca ggagctggat aagtataaga
     2761 acgccgttac cgaactgcag ctgctgatgc agtccacccc cgccgctaat tcccgcgcca
     2821 gacgggaact gccccgcttc atgaactaca ccctgaataa cactaagaac accaacgtga
     2881 cgctttccaa gaagaggaag aggaggttcc tggggttcct gctgggcgtg ggcagcgcca
     2941 tcgccagcgg aattgccgtg agcaaggtgc tgcatctgga gggcgaggtc aacaagatta
     3001 agtcagccct gctgagcacc aacaaggccg tggtgtccct gagcaacggt gtgagcgtgc
     3061 tgacaagcaa agtgctggac ctgaagaact acattgacaa gcagctgctg ccaatcgtca
     3121 ataagcagag ttgctccatc agcaatattg agactgtgat cgagttccag cagaagaata
     3181 accgcctgct ggagattacc agggagttca gcgtgaatgc aggcgtgacc acccccgtgt
     3241 ccacctatat gctgactaat agcgagctgc tgagcctgat caacgatatg ccaatcacca
     3301 acgaccagaa gaagctgatg tcatccaatg tgcagattgt gcgccagcag agctacagca
     3361 ttatgtccat catcaaggaa gaggtgctgg cttacgtggt gcagctgcct ctgtacggag
     3421 tgatcgatac cccatgctgg aagctccaca cctctccact ctgtactacc aacacaaagg
     3481 agggcagcaa catctgtctg acccgcaccg atcgcggatg gtactgcgac aacgctggca
     3541 gcgtgagctt ctttccccag gccgagactt gtaaggtgca gagcaacagg gtgttctgtg
     3601 ataccatgaa tagtctgacc ctcccctctg aggtgaatct gtgcaacatc gacatcttca
     3661 accccaagta cgattgtaag atcatgacat ctaagaccga tgtgagtagc agcgtgatca
     3721 cttccctggg cgccatcgtg agctgctacg gcaagaccaa atgcaccgcc tctaataaga
     3781 acaggggtat catcaagaca tttagcaacg ggtgtgacta cgtgtctaat aagggggtgg
     3841 ataccgtgag tgtgggcaac accctgtatt acgtgaacaa gcaggaaggc aaatccctgt
     3901 acgtgaaagg cgaacctatc atcaacttct acgacccact ggtgttcccc agcgacgagt
     3961 tcgatgcctc catcagccag gtgaatgaga agatcaacca gagcctggct tttatcagaa
     4021 agtctgacga gctgctgcac aacgtgaatg ccggaaagtc caccactaat attatgatca
     4081 ccactataat cattgtgatc atcgtgattc tgctgtccct gatcgctgtg ggtctgctgc
     4141 tgtactgcaa ggctcgctcc actcccgtga cactcagcaa ggaccagctg agcggcatca
     4201 acaatatcgc attttccaat taatgaaagc ttatcgatac cgtcgacctc gagggcccag
     4261 atctaattca ccccaccagt gcaggctgcc tatcagaaag tggtggctgg tgtggctaat
     4321 gccctggccc acaagtatca ctaagctcgc tttcttgctg tccaatttct attaaaggtt
     4381 cctttgttcc ctaagtccaa ctactaaact gggggatatt atgaagggcc ttgagcatct
     4441 ggattctgcc taataaaaaa catttatttt cattgcaatg atgtatttaa attatttctg
     4501 aatattttac taaaaaggga atgtgggagg tcagtgcatt taaaacataa agaaatgaag
     4561 agctagttca aaccttggga aaatacacta tatcttaaac tccatgaaag aaggtgaggc
     4621 tgcaaacagc taatgcacat tggcaacagc ccctgatgcc tatgccttat tcatccctca
     4681 gaaaaggatt caagtagagg cttgatttgg aggttaaagt tttgctatgc tgtattttac
     4741 attacttatt gttttagctg tcctcatgaa tgtcttttca ctacccattt gcttatcctg
     4801 catctctcag ccttgactcc actcagttct cttgcttaga gataccacct ttcccctgaa
     4861 gtgttccttc catgttttac ggcgagatgg tttctcctcg cctggccact cagccttagt
     4921 tgtctctgtt gtcttataga ggtctacttg aagaaggaaa aacagggggc atggtttgac
     4981 tgtcctgtga gcccttcttc cctgcctccc ccactcacag tgacccggaa tccctcgaca
     5041 tggcagtcta gatcattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
     5101 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     5161 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     5221 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     5281 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     5341 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     5401 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     5461 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     5521 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     5581 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     5641 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     5701 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     5761 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     5821 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     5881 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     5941 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     6001 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     6061 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     6121 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     6181 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     6241 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttc
//
