LOCUS       sequence-469326-       11209 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of NM2C (dog) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11209)
  AUTHORS   Rouaud F, Huang W, Flinois A, Jain K, Vasileva E, Di Mattia T, 
            Mauperin M, Parry DAD, Dugina V, Chaponnier C, Mean I, Montessuit S,
            Mutero-Maeda A, Yan J, Citi S
  TITLE     Cingulin and paracingulin tether myosins-2 to junctions to 
            mechanoregulate the plasma membrane.
  JOURNAL   J Cell Biol. 2023 Jul 3;222(7):e202208065. doi: 
            10.1083/jcb.202208065. Epub 2023 May 19.
  PUBMED    37204781
REFERENCE   2  (bases 1 to 11209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-07-3"; volume: "222(7):e202208065. doi"; pages: 
            " 10.1083/jcb.202208065. Epub 2023 May 19"
COMMENT     Sequence Label: HA-cNM2C
FEATURES             Location/Qualifiers
     source          1..11209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1040..1161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1051..1070)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1105..1124
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1291..1665)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(1684..1731)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(1779..2108)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1793..1928
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2093..2113
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2122..2550)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2232..2253)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2444..2463
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    2596..2820
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2809..2826
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(9073..9099)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(9161..9180)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9162..9180)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(9225..9428)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9254..9274)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9429..9808
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     9980..9999
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        10074..10178
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10179..10247,10248..11039)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10397..10416)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtagcacgtg tcagtcctgc tcctcggcca cgaagtgcac
     1321 gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct cgccgatctc
     1381 ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac acgacctccg accactcggc
     1441 gtacagctcg tccaggccgc gcacccacac ccaggccagg gtgttgtccg gcaccacctg
     1501 gtcctggacc gcgctgatga acagggtcac gtcgtcccgg accacaccgg cgaagtcgtc
     1561 ctccacgaag tcccgggaga acccgagccg gtcggtccag aactcgaccg ctccggcgac
     1621 gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg gccatggttt agttcctcac
     1681 cttgtcgtat tatactatgc cgatatacta tgccgatgat taattgtcaa cacgtgctga
     1741 tcagatccga aaatggatat acaagctccc gggagctttt tgcaaaagcc taggcctcca
     1801 aaaaagcctc ctcactactt ctggaatagc tcagaggcag aggcggcctc ggcctctgca
     1861 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
     1921 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
     1981 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
     2041 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
     2101 tgacacacat tccacagaat taattcgcgt taaatttttg ttaaatcagc tcatttttta
     2161 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     2221 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     2281 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     2341 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     2401 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     2461 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     2521 ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc cctagagccc cagctggttc
     2581 tttccgcctc agaagccata gagcccaccg catccccagc atgcctgcta ttgtcttccc
     2641 aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca
     2701 gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca
     2761 gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag
     2821 tcgaggctga tcagcgggtt taaacgggcc ctctagactc gagcggcctt actgcggttg
     2881 ggacaccggg ggcccttccg gctccggggg cggcccggcc ccaggctccg cctcctcgac
     2941 ctcctcttct gatgccacgc cctcctccag cctgaagacc tggcgcacag ttcgagtggt
     3001 gaaggtaagg ggaccgcgcc ggagccggtt cctcagcgtg ttcaactcgc ggttcatgga
     3061 ctccgaggcc tctgtgacat cttctagctc gtgctgcagc ctccggcgga ccgactgagc
     3121 ccgggacgcc tcctcctcgg cctcctccag ctgccgcttc agctgcttca cccggaggtt
     3181 gcttttctcc agctggtccc gaagctggtc agccaccctc cgctcctcct ccacttggag
     3241 taccacctct ttaagccgct tctcagccct gcgcaccaac ttgcctgaga ggatgcgctc
     3301 cctactctcc tgctccagct gttcctcagc ctgggccagc ttggattcca gggcggcaat
     3361 gaccatcttc tggcgggccc gagccccagc atcctcctca cctaggcgcc cccggagctc
     3421 ctggatctgc cgctccagct gctgtcgccc gctctctgcc ttggctgaga aactacgctc
     3481 agctgacaat tctgtagtca gtgattccac ctgcaggagc agcttgcggt agcggtcatt
     3541 gagcagctcc gcattgctct gctcctcctc cagctcttct tccatttgac ccaggcgccc
     3601 ctccagctga cgcttctctt ccagaatagc cgtcttgcta aggctgccat tggccacctc
     3661 gtctgccatc tcatcccgct cctgctggat ttgccgccgg gcacgatctg aggcggccag
     3721 ttcctcctgc agccgcagca cctctgcctc cagcgccttg aggcgtttct cattctcccg
     3781 gttctgggca aagatttcct cccgggagct gcgcgtctct tccacctccc gccacagctc
     3841 cttcatctgg gtctgcatct tgcggagctg cttcaccgct tcctccttgc cctgcacccc
     3901 catcgcattc tgggctctca agtcctctac ttcggcctcc agcttcttgc gggcggccac
     3961 agccagggcg cgttgcttgc gttcctcatc ccgctccacc tctgcatccc tcagctgctt
     4021 ggccagctgc cgccgcctct cctcgccagc ctcatctcgg ctctgcaggt cgcgctcatg
     4081 ctgtgccttg agagcctgca cggtcacttc cagccgcagc ttggcgtcct cggctgccgt
     4141 cagctcatcc tccagctcgg tcacctgtgc tcgcaggtcg ttggctgtct gttctgctgc
     4201 ccggcgggat cgctccagct catgcgcgtt cttgccgacg tcatccttac tgctcagcag
     4261 tgcttccatc tctgcccgca gggcccggtt ctgccgctcc agctcctcac gtgcttcctg
     4321 ctcctcctcc agtgcccgag tcagtgacag ggtccgagcc tcgcgctccc ggccctccgc
     4381 ctcaacccgc tcacgttcct ccaccgcccg caggacagct gccttctcct ctgccaggag
     4441 ctggtcgaac ttgcgctgct tcttctccag tgtgctcacg agctgccgct gctgctccag
     4501 gtccacggtg gcatcgtcca gctcctgccg cagctggcgg cggccccgct ccagccgctc
     4561 caccgcctca gttttttctg ccaggcgcag gcttagggcc tcagcctcac gggctgcccg
     4621 gcgccgggcc tcctctcctg cttccagggc tcctgcctcc tcctcctggc gccgccgcca
     4681 ctctgagagc tgggcctggg cggtctgtag ctcgcggcct gcccgctccc tggcggctgc
     4741 ctcctcctcc agctgttccc gcagccccgc cacttcagcc tccagggctc gcgccttgga
     4801 ccccaaggcc agctttgccc tactctcctc ctgaagcatc tcctgagcgt catgtagctg
     4861 ggcctctgag ctgctcagct ccttgctgag ccggatggct ctggactcgg cctcactcag
     4921 ggccccggac acattctcca gttcagcctg ggctcgctga agcttctcag cagcctcggc
     4981 tcgcgcccgc tccccgtcgc cagcccggcc ctgaacttcc tgcagctgtg actccaagcg
     5041 gcgtctccgc tgctcgccct cgtgacgtga ggcctgcagg ctgctcagct ccgaccgtag
     5101 ctcagacact tcagtctcca gggccaaccg ggtcttctcc catgtacctt tgctcctccg
     5161 ggcctgttcc agttgctccg ccagctcgcc caatgcctgc ccatggcgct gcctcagctc
     5221 ctgcaccgcc acttcgtgaa cacgtgtctc ctcttccaga gccttcttca actctgtcac
     5281 ttcctgttcc ctcttggacc tgagctcctg ctgcgcattg gtggagtcca gcgtgtcctc
     5341 tagttcactc cgcagcgcct ccagctcctc acccaggtcc ctgcgctgct tctctgcctt
     5401 ggccctggct gcccgctcgg cctccaggtc ttcctgggcc tccgcgagtc ctgcctgtgc
     5461 ttcccgcagg gatttgagca gctgggcccg ggcaccgccc tcctcctcca ccctggccag
     5521 ggccgcctgc agctcctcct ccttgcggcc cagctgggcc cgcagctcct ccccacgctg
     5581 ctgctgctcg gccatctgct cctgcagctc cgagctctcc ccgtccagcc gccgcttcag
     5641 cttctccagc tcctggcggc ccttctcctc cttccgtagg cggtcttcca tgtccgcaat
     5701 cgtggcctcg tatttgagtc gaagcttatt gaggctcttg accttctcct cctcttcggc
     5761 cgcctgtgat gagaactcgg ccagacgctc ctccaacagc ttccgctcct tgctcagctt
     5821 ggcattctga tcttctaaaa gtagcaggtc ttcctcaaat ttcttcagct tcgcctctgt
     5881 ggtcaccttc tccagctgca gtttctgcct tgccccttcc tcagcctcga ggtggctctc
     5941 cagatcctgt atatgctgct gcagcctctt cttgtcactt tgcagctggc gggtgcactc
     6001 ctcctcctcg cccacacggg cctccagctc tgataccacc agctccagct cctgtttgcg
     6061 ggctgccagc cgcccccgtg tctcttcagc ctccgcacac agctctgcct ctgctcgcag
     6121 ttgttctgcc aggcgagtgc gctcctcttc cagctgcgcc aggcggccct ggagctcgcc
     6181 cacctcgcgg gcgctctgct gctgcagctc ctgaactttc tgtagctcct gggcccgcgc
     6241 ctgcagcacc tcatcctgcc gcgtcacctg cagcagcggc ttcaccttga tgaagagccg
     6301 ccaccactgc cagtgtctca gcttgaggta agcagcacag ttcctctgca tcaccctcag
     6361 ggcactctgc tgctgctgcc gcttctggaa ggctctgcga gctaggtacc cccgggcagc
     6421 tgcctggaag gacacgatga tgtctgtgac cttcaggtcc cgttcctctt ccagctgggc
     6481 caggacccct gcccggaaga agatcttgct ctgtcctaca cggtagaggt tggggtccag
     6541 ttctagagcc tggatcatct tctcacaggc ctgtttccca tccataaagc ccttggggat
     6601 ggcattgggc gtcaggatct cgtatcgctg ccggaactcc tggaagagga tgcggttggg
     6661 gaagccctgg cgacagatcc ggatgccctc caggacgccg ttgcagcgca gctggtccag
     6721 caccagccga ggctccagct tgccagccct cttctcatgg ttggggacga tacagcgaac
     6781 gaagctgggg ttggtgttgc tgagtgtggc catgagacgg ctcagtgact ccttgtagag
     6841 ctgtcctact gttcggaaca tgccccggcg ggggcggcca cccggagggc catctccaag
     6901 gctgctcacc tgctccagcc ccacgatccc ctccacccct gttggagaaa tggcagagcc
     6961 gtgcctccct gaagatcctg ggggtgatgg tgggagggag ccaaggaaag agaactgctg
     7021 gaagcccccg tgttcatctt tccagatctc tgccgtcagc ttgtctgtgc tcatgtgaag
     7081 cagggctgcc acgttgtcat tcagagggtc catgtttttc atcagccact cattggcctt
     7141 gtagtcgacc ttgcccgcat agtgcaggac gctgaagtca gcctggtccc gcaggttcct
     7201 cggccgctga aacttgggat ggcttccttg ctcctgggcc accttctcta caaaggactt
     7261 gtccgtggcc tttgggaacc agcactcctc gtccagcagg gccaggagcc caggggggtt
     7321 agccggccgc tcaatgaggt caatgcaggg ctgtaggtcg aggccaaagt cgaggaaggt
     7381 ccaggggatg ccctcgcgct ggtactcctc ctgctccagg atgaacatgg tgtggttgaa
     7441 gagctgctgc agcttctcgt tggtgtagtt gatgcagagc tgctcgaagg agttgagctg
     7501 gaagatctca aagccagcga tgtccaggat ccccaggaaa gaggcgccct ggcggggact
     7561 gcggtccagg gcccggttga ggcgcaggac cagccagcgg aagaggcgct cataggtggc
     7621 cttggccagg gcctccagtg caaagtcagc ctgttccttg gtctgtgctt tctgcacgta
     7681 gtctcggcca actttgatgc ggggggtgag cagggctcgg gagaagtccg tcacccctac
     7741 tcccaggagg cggcagagct tctgtgcagc tgtgttgtca ggcatggagg cttgatcctt
     7801 attccgttct ctcttcagga cgatgttgcc aaactggaga acggcagaga ccatccgcag
     7861 catggaggtg atttcctccg gggtgaatcc caggaccttc agtgactcca gcgtctcgtg
     7921 gaagagctcc cgctcctggc caggagagga tgacggcccg ttggtcagga agcggtagtt
     7981 ggagcatggc tccaagagga gttcagcttt gagatgctct ccagcacctc ccagcagctg
     8041 atagaagatg tggaagctgc actcatcttt ggcctggcgg atggccctgg acttctccaa
     8101 caggtaggtc tcgatgttgg cgcccacaat gtacccagcc acgtcgaagt tgatgcgaat
     8161 aaatttgcca aatcgggaag agttgtcatt cttcacagtc ttggcgttgc caaaggcctc
     8221 taggatgggg ttggcctgaa gcagctgccg ctccagctca ccataagaca cgatgctggc
     8281 agccggtaca cctggctcct tcctgccctt tggtgatgat gccacgtggg caagatactg
     8341 gatgaccttc ttggtgtttt ctgtcttccc agccccagat tccccggtgc agagaatgga
     8401 ctgatcttca cgatcctgga gcatgcttcg gtaggccccc tcggtcaccg catacacgtg
     8461 gggtggcact tcatggcgct tcttgccccg gtacatctcc acaatggcct ctgtatagat
     8521 gggaagctgc ttgtacggat tgatgaccac acagaaaagg ccagagtacg tatagatgag
     8581 gcccgagtag taccgctcac ggaggttgtg cagcaccgag gcctcgttga ggcaggtgag
     8641 ctccgccatg tcctcggcct tgctgaactt gggcgggttc atgcgctgga tttggtcccg
     8701 cggcagccgc agccgccgcc cgctctcggc cagctccact tcggcctcct cctcgccctc
     8761 gtgccgcagc gccgccgcct cgaacccgtg tagctccgaa ggcacccaca ccaggcgccg
     8821 cgccgtccac tccacctgcg gcgaggtgcc tgcgcccccg ggcgcgcccc ccatgcctgg
     8881 cccactgggc gtgaacaggt agggctgggc ggcctccggc accgggctgg gcctgggggc
     8941 caccttccgc ccaggcaccg acaaggtcgc tgcggccatg gtctgctggt ctctcccgct
     9001 gggctgggag ctcttccagg acacaggcct gagtcggttt gccttggctt ccccagagcc
     9061 cagcatatcg atagcgtagt ctgggacgtc gtatgggtac atggtggcgg atccgagctc
     9121 ggtaccaagc ttaagtttaa acgctagcca gcttgggtct ccctatagtg agtcgtatta
     9181 atttcgataa gccagtaagc agtgggttct ctagttagcc agagagctct gcttatatag
     9241 acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat
     9301 tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg
     9361 agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac
     9421 cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc
     9481 cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata
     9541 gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa
     9601 tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc
     9661 attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta
     9721 agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac
     9781 tattaataac tagtcaataa tcaatgtcaa cgcgtatatc tggcccgtac atcgcgaagc
     9841 agcgcaaaac gcctaaccct aagcagattc ttcatgcaat tgtcggtcaa gccttgcctt
     9901 gttgtagctt aaattttgct cgcgcactac tcagcgacct ccaacacaca agcagggagc
     9961 agatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatagggga
    10021 tcgggagatc tcccgatccg tcgacgtcag gtggcacttt tcggggaaat gtgcgcggaa
    10081 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
    10141 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
    10201 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
    10261 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
    10321 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
    10381 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
    10441 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
    10501 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
    10561 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
    10621 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
    10681 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
    10741 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
    10801 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
    10861 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
    10921 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
    10981 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
    11041 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
    11101 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
    11161 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttc
//
