LOCUS       sequence-469327-        3925 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; ConLS/R1 cassette; rtTA systems; pRAD27-rtTA-v16-tADH1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pRO247
FEATURES             Location/Qualifiers
     source          1..3925
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(103..894,895..963))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     726..745
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(964..1068)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1171..1759
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1660..1679
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      3524..3711
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3579..3603)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 gcggccgcga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
       61 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
      121 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
      181 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
      241 gcgggaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
      301 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
      361 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
      421 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
      481 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
      541 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
      601 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
      661 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
      721 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
      781 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
      841 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
      901 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
      961 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     1021 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     1081 aaaagtgcca cctgtcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1141 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1201 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1261 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1321 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1381 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1441 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1501 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1561 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1621 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     1681 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     1741 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     1801 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtgc
     1861 ggccgcccct gaattcgcat ctagatggta gagccacaaa cagccggtac aagcaacgat
     1921 ctccaggacc atctgaatca tgcgcggatg acacgaactc acgacggcga tcacagacat
     1981 taacccacag tacagacact gcgacaacgt ggcaattcgt cgcaataccg tctcactgaa
     2041 ctggccgata attgcagacg aacgccttgt gaaattgcaa atatggtgat ttgaaacgtt
     2101 tcctagtgca gcaggatcac agataacgtg taaagggctt agcagttgat aatcctctct
     2161 agttaagacc taaacaaaat gctgtcacta accgtagtat taaatgacac actttggtga
     2221 ctttcgttaa tggggatgtg gtagtggcca ttgccaataa acaaaaagaa cagggaaaga
     2281 agtagaaagt gatataagtt tgcttgccac ttttcgtttt tcacgaaaaa aacaggcgaa
     2341 aaaaaatgct agacaagtac ccggctgaat cacacctcgt taacagtgac tttcggtgac
     2401 agatacccga ttgggcaccc ggctggtaag ttatgataga aagccaacgc tgtactattg
     2461 gcttagctat ggcaatattt tgattatcag ctagttttat taacgttata attagtgtaa
     2521 ccagtttttc atctatttca tttatttcat ttatttactt taattgcaga tccccctaac
     2581 gcgtttaaag cttttattca ctagcttatg tattttttat aggaaacgcg acgcgtaaca
     2641 tcgcgcaaat gaaggttttg atgtattata atgaggtatt cttccttata tacatcgatg
     2701 aaaagcgttg acagcataca ttggaaagaa ataggaaacg gacaccggaa gaaaaaatag
     2761 atctatgagt cgtcttgata agtccaagat cataaatagc gcactggaat tgctgaacgg
     2821 cgttggtatt gagggtctta ccacgagaaa gctggcacag aagttggggg tcgaacaacc
     2881 gaccttgtac tggcatgtca agaataagcg tgcgctatta gatgccttac ctatcgagat
     2941 gctagacaga catcatactc actcatgtcc attggagggg gagtcttggc aagactttct
     3001 tagaaacaat gcaaagtcat ataggtgcgc gctactatca catagagatg gggccaaagt
     3061 ccatttgggc accaggccga ccgaaaagca gtacgagact ttagagaacc agctggcatt
     3121 cctgtgtcaa caaggcttca gtctagagaa tgctctgtat gctttaagcg cggtcggcca
     3181 cttcacgcta ggttgtgtat tagaggaaca agaacaccaa gtggcaaaag aggaaagaga
     3241 aactccaacg accgacagca tgccaccact gttgaaacaa gcaatcgaac tgtttgatcg
     3301 tcaaggggct gaaccagcat tcctattcgg tttggaatta ataatctgcg gtctggagaa
     3361 acaattgaag tgcgaaagcg gcgggccgac agatgcctta gatgactttg acctagatat
     3421 gttaccagcc gacgcccttg acgactttga tctggacatg ctgccggctg acgccttgga
     3481 tgacttcgac ttggatatgt tgccaggggg atcctaactc gaggcgaatt tcttatgatt
     3541 tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat tttaaagtga
     3601 ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac tctttcctgt aggtcaggtt
     3661 gctttctcag gtatagcatg aggtcgctct tattgaccac acctctaccg gcatgccgag
     3721 caaatgcctg caaatcgctc cccatttcgc tgccaatgag acgacggggt catcacggct
     3781 catcatgcgc caaacaaatg tgtgcaatac acgctcggat gactgcatga tgaccgcact
     3841 gactggggac agcagatcca cctaagcctg tgagagaagc agacacccga cagatcaagg
     3901 cagttaacta gtgcactgca gtaca
//
