LOCUS       sequence-469328-        4355 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; ConLS/R1 cassette; ABA systems; 
            pTDH3-Phlf-NES-PYL1-tADH1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pRO241
FEATURES             Location/Qualifiers
     source          1..4355
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        220..886
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     756..779
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             1502..1528
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     terminator      2096..2283
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2151..2175)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(join(2600..3391,3392..3460))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3223..3242
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3461..3565)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3668..4256
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4157..4176
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcggccgccc ctgaattcgc atctagatgg tagagccaca aacagccggt acaagcaacg
       61 atctccagga ccatctgaat catgcgcgga tgacacgaac tcacgacggc gatcacagac
      121 attaacccac agtacagaca ctgcgacaac gtggcaattc gtcgcaatac cgtctcactg
      181 aactggccga taattgcaga cgaacgcagt tcgagtttat cattatcaat actgccattt
      241 caaagaatac gtaaataatt aatagtagtg attttcctaa ctttatttag tcaaaaaatt
      301 agccttttaa ttctgctgta acccgtacat gcccaaaata gggggcgggt tacacagaat
      361 atataacatc gtaggtgtct gggtgaacag tttattcctg gcatccacta aatataatgg
      421 agcccgcttt ttaagctggc atccagaaaa aaaaagaatc ccagcaccaa aatattgttt
      481 tcttcaccaa ccatcagttc ataggtccat tctcttagcg caactacaga gaacaggggc
      541 acaaacaggc aaaaaacggg cacaacctca atggagtgat gcaacctgcc tggagtaaat
      601 gatgacacaa ggcaattgac ccacgcatgt atctatctca ttttcttaca ccttctatta
      661 ccttctgctc tctctgattt ggaaaaagct gaaaaaaaag gttgaaacca gttccctgaa
      721 attattcccc tacttgacta ataagtatat aaagacggta ggtattgatt gtaattctgt
      781 aaatctattt cttaaacttc ttaaattcta cttttatagt tagtcttttt tttagtttta
      841 aaacaccaag aacttagttt cgaataaaca cacataaaca aacaaaagat ctatggcacg
      901 tacgccctca cgttccagca taggtagcct tagatcacca catacgcaca aagccatcct
      961 gacttcaact attgaaatac taaaagaatg cgggtattct ggactaagta tcgaatcagt
     1021 ggctagacgt gccggggcca gcaaacccac aatttaccgt tggtggacca acaaagccgc
     1081 tctaatagcc gaagtgtacg aaaacgagtc tgaacaggtc cgtaagttcc ctgatttagg
     1141 ctcctttaag gcggatttgg atttcttact gagaaaccta tggaaagtat ggagggagac
     1201 aatttgcgga gaagcattta ggtgcgtcat tgctgaagcc caacttgatc ccgcgacgct
     1261 tacacaacta aaagatcagt ttatggagag aaggcgtgaa atgccaaaga aattagtaga
     1321 aaacgccata tcaaacgggg agctacccaa agacaccaac agagaactgc tattagatat
     1381 gatctttggt ttttgctggt acagactgtt gactgaacaa cttactgtgg agcaagatat
     1441 agaggagttc acctttctgt tgataaacgg agtttgccca gggacacaac gtggaagcgg
     1501 actgcctccg ttagaaagac taacgctggg ttctggtgga ggtgctccaa cacaagatga
     1561 gttcacacaa ctatcacaga gtatcgcaga attccatacc taccaactag gaaatggaag
     1621 atgttcttca ctgcttgcgc aaagaatcca tgccccgccg gagactgtgt ggagtgtagt
     1681 taggaggttc gacaggccgc agatatacaa gcactttatc aaatcctgca atgttagcga
     1741 agatttcgag atgcgtgttg gatgcaccag ggacgtaaat gttatatctg gattacctgc
     1801 gaatacgtca agagaaaggt tggacttact agacgacgac agacgtgtta ccggattttc
     1861 cataacgggt ggtgaacatc gtctacgtaa ttataagagc gtgactacag tccaccgttt
     1921 tgaaaaggag gaagaggagg agagaatatg gaccgtggtt ctggaatcct acgttgttga
     1981 tgtacccgag gggaatagcg aggaggatac acgtctgttc gcggataccg ttatcagatt
     2041 gaatcttcaa aaacttgctt ccattacaga ggccatgaac ggatcctaac tcgaggcgaa
     2101 tttcttatga tttatgattt ttattattaa ataagttata aaaaaaataa gtgtatacaa
     2161 attttaaagt gactcttagg ttttaaaacg aaaattctta ttcttgagta actctttcct
     2221 gtaggtcagg ttgctttctc aggtatagca tgaggtcgct cttattgacc acacctctac
     2281 cggcatgccg agcaaatgcc tgcaaatcgc tccccatttc gctgccaatg agacgacggg
     2341 gtcatcacgg ctcatcatgc gccaaacaaa tgtgtgcaat acacgctcgg atgactgcat
     2401 gatgaccgca ctgactgggg acagcagatc cacctaagcc tgtgagagaa gcagacaccc
     2461 gacagatcaa ggcagttaac tagtgcactg cagtacagcg gccgcgatta tcaaaaagga
     2521 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     2581 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     2641 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     2701 agggcttacc atctggcccc agtgctgcaa tgataccgcg ggacccacgc tcaccggctc
     2761 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     2821 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     2881 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     2941 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     3001 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     3061 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     3121 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     3181 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     3241 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     3301 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     3361 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     3421 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     3481 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     3541 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gtcatgacca
     3601 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3661 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3721 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3781 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     3841 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3901 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3961 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4021 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     4081 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     4141 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4201 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4261 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4321 ttcctgcgtt atcccctgat tctgtggata accgt
//
