LOCUS       sequence-469329-        4214 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; ConL1/R2 cassette; ABA systems; pTDH3-NLS-VP48-ABI-tENO1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pRO204
FEATURES             Location/Qualifiers
     source          1..4214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        220..886
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     756..779
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             896..916
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(2459..3250,3251..3319))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3082..3101
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3320..3424)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3527..4115
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4016..4035
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcggccgccc ctgaattcgc atctagaacg gggtcatcac ggctcatcat gcgccaaaca
       61 aatgtgtgca atacacgctc ggatgactgc atgatgaccg cactgactgg ggacagcaga
      121 tccacctaag cctgtgagag aagcagacac ccgacagatc aaggcagtta cgtctcacca
      181 aaccagatgt caacacagct acaacgcagt tcgagtttat cattatcaat actgccattt
      241 caaagaatac gtaaataatt aatagtagtg attttcctaa ctttatttag tcaaaaaatt
      301 agccttttaa ttctgctgta acccgtacat gcccaaaata gggggcgggt tacacagaat
      361 atataacatc gtaggtgtct gggtgaacag tttattcctg gcatccacta aatataatgg
      421 agcccgcttt ttaagctggc atccagaaaa aaaaagaatc ccagcaccaa aatattgttt
      481 tcttcaccaa ccatcagttc ataggtccat tctcttagcg caactacaga gaacaggggc
      541 acaaacaggc aaaaaacggg cacaacctca atggagtgat gcaacctgcc tggagtaaat
      601 gatgacacaa ggcaattgac ccacgcatgt atctatctca ttttcttaca ccttctatta
      661 ccttctgctc tctctgattt ggaaaaagct gaaaaaaaag gttgaaacca gttccctgaa
      721 attattcccc tacttgacta ataagtatat aaagacggta ggtattgatt gtaattctgt
      781 aaatctattt cttaaacttc ttaaattcta cttttatagt tagtcttttt tttagtttta
      841 aaacaccaag aacttagttt cgaataaaca cacataaaca aacaaaagat ctatgcccaa
      901 gaagaagcgt aaagtgccgg cggatgctct agacgatttc gatctggata tgctaccagc
      961 cgatgctctt gacgacttcg acttagacat gctaccggcc gacgctctag atgacttcga
     1021 cttagacatg ctcggttcta cgagagttcc tttgtatggc ttcacctcaa tttgcggtag
     1081 aagacccgag atggaagccg ccgtatcgac gataccacgt tttttacaat cctcctccgg
     1141 ctcaatgtta gacggacgtt ttgatcccca gagtgcagct catttctttg gtgtttatga
     1201 tggtcacggg ggtagccagg tagccaacta ttgccgtgaa aggatgcatt tagcgttagc
     1261 tgaggaaatc gccaaagaaa agcctatgtt gtgtgatgga gacacctggt tggagaagtg
     1321 gaaaaaggcg ttattcaaca gcttcctaag ggtggatagt gagatagagt ctgttgctcc
     1381 ggagactgtt ggctcaacat ccgtggtggc cgtggtcttc ccgagccata tttttgtggc
     1441 taattgtggt gactctcgtg cggtcttgtg cagggggaag actgcattac ctttaagcgt
     1501 cgatcataag ccggacaggg aggacgaagc agcgaggata gaggccgccg gtggaaaggt
     1561 aatacaatgg aatggtgcta gggtattcgg cgtactggca atgagtagat ccattggtga
     1621 tagatacttg aagccgagca taatccctga ccctgaagta acagcggtta aaagggtgaa
     1681 ggaagacgat tgccttatct tggcttctga cggagtgtgg gatgtcatga ctgacgagga
     1741 agcctgcgaa atggcaagga aaagaatact actttggcat aagaaaaatg cggtagctgg
     1801 ggacgcaagt ctgttagcag atgaaagaag aaaagaaggc aaggaccccg ccgcaatgtc
     1861 agccgcggag tacctgtcta aacttgctat tcagaggggg agtaaagata acatatccgt
     1921 ggtcgtggtt gatctaaagg gatcctaact cgagagcttt tgattaagcc ttctagtcca
     1981 aaaaacacgt ttttttgtca tttatttcat tttcttagaa tagtttagtt tattcatttt
     2041 atagtcacga atgttttatg attctatata gggttgcaaa caagcatttt tcattttatg
     2101 ttaaaacaat ttcaggttta ccttttattc tgcttgtggt gacgcgtgta tccgcccgct
     2161 cttttggtca cccatgtatg ctggatgtga gacgataaat cgcagccaag tgagtgaata
     2221 gatgacgcac cacggtcaga cacggccaca tcgtatctca caggagcaag cgcgatagga
     2281 gcactcacac atagtacggt gatccgctga ctcctttgcc caaataagac gtgagccact
     2341 agtgcactgc agtacagcgg ccgcgattat caaaaaggat cttcacctag atccttttaa
     2401 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     2461 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     2521 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     2581 gtgctgcaat gataccgcgg gacccacgct caccggctcc agatttatca gcaataaacc
     2641 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     2701 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     2761 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     2821 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     2881 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     2941 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     3001 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     3061 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     3121 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     3181 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     3241 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     3301 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     3361 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     3421 cgcgcacatt tccccgaaaa gtgccacctg tcatgaccaa aatcccttaa cgtgagtttt
     3481 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3541 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3601 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3661 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3721 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3781 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3841 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3901 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3961 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     4021 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     4081 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     4141 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     4201 ctgtggataa ccgt
//
