LOCUS       sequence-469330-        2544 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; type3B; NS3 systems; NS3-V1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pAL009
FEATURES             Location/Qualifiers
     source          1..2544
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             863..889
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      complement(909..943)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1014..1673)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1588..1607
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1855..2443
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2344..2363
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat tctggggcga gcgcgggcat ggcgtcaatg actgggggcc agcaaatggg
       61 gagtacggag gatgtagttt gttgtcattc aatatacggc aagaagaagg gtgacatcga
      121 cacgtacaga tacataggct ctagtgggac aggctgcgta gtaatcgtgg gcagaattgt
      181 cctaagcgga tccggaacca gtgcgcctat caccgcctac gcacaacaaa ccaggggact
      241 attgggctgt atcataacaa gcctaactgg aagagacaaa aatcaagtcg agggcgaggt
      301 tcagatcgta tctacggcaa ctcagacctt tcttgccacg tgcataaacg gcgtgtgctg
      361 ggccgtatat catggggctg gcacaagaac gatagcgagt cctaagggtc ctgttattca
      421 aatgtatacc aacgtagacc aggatctagt cggctggccc gcgccccaag gctctaggag
      481 tcttacccct tgcacgtgcg ggtcctcaga cttatacctg gtaacgaggc acgcagatgt
      541 tatacctgtg cgtagaaggg gtgacagtag aggatctctt ttatcccccc gtcccattag
      601 ctacttaaag ggtagctctg gggggccgtt gttatgtccg gcaggacatg ccgtcgggtt
      661 attcagagcg gctgtttgta cgaggggtgt tgcaaaagca gtagatttta taccggttga
      721 gaacttagag acaacgatga ggtccccggt ttttacagac aactcatctc cacctgctgt
      781 tacactgacc caccccatca ccaaaattga cagggaggtt ctctaccagg aatttgacga
      841 aatggaggag tgctcacaac actatccata cgacgtacca gattatgctg gatcctgaga
      901 ccagaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt
      961 ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc
     1021 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
     1081 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
     1141 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
     1201 aaatcaaaac tggtgaaact cacccaggga ttggctgaaa cgaaaaacat attctcaata
     1261 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
     1321 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
     1381 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
     1441 ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
     1501 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
     1561 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
     1621 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct
     1681 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca
     1741 ttatggtgaa agttggaacc tcttacgtgc ccgatcaatc atgaccaaaa tcccttaacg
     1801 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     1861 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1921 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     1981 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     2041 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     2101 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     2161 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     2221 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2281 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2341 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2401 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2461 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     2521 ccctgattct gtggataacc gtag
//
