LOCUS       sequence-469331-        1806 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; type3A; Activation domains; NLS-VP48.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pRO176
FEATURES             Location/Qualifiers
     source          1..1806
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             14..34
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      complement(171..205)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(276..935)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     850..869
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1117..1705
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1606..1625
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat atgcccaaga agaagcgtaa agtgccggcg gatgctctag acgatttcga
       61 tctggatatg ctaccagccg atgctcttga cgacttcgac ttagacatgc taccggccga
      121 cgctctagat gacttcgact tagacatgct cggttcttga gaccagacca ataaaaaacg
      181 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
      241 ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg
      301 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga
      361 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg
      421 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
      481 ctcacccagg gattggctga aacgaaaaac atattctcaa taaacccttt agggaaatag
      541 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
      601 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
      661 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat
      721 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
      781 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
      841 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
      901 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
      961 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
     1021 cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1081 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1141 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1201 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     1261 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     1321 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     1381 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     1441 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     1501 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     1561 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     1621 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     1681 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     1741 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     1801 ccgtag
//
