LOCUS       sequence-469332-        1963 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; type4A; Activation domains; VP48.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pRO288
FEATURES             Location/Qualifiers
     source          1..1963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             182..841
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(248..267)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      912..946
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     rep_origin      complement(1375..1963)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1455..1474)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgattga tcgggcacgt aagaggttcc aactttcacc ataatgaaat
      121 aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa
      181 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
      241 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
      301 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
      361 tcacattctt gcccgcctga tgaatgctca tccggaattt cgtatggcaa tgaaagacgg
      421 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
      481 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
      541 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
      601 gaatatgttt ttcgtttcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
      661 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
      721 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca
      781 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta
      841 atttgatatc gagctcgctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc
      901 atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt ctggtctcaa
      961 tccccggcgg atgctctaga cgatttcgat ctggatatgc taccagccga tgcacttgac
     1021 gatttcgact tagacatgct tccggccgac gctctagatg acttcgactt agatatgctc
     1081 taatggctga gacctgagac ggcatccgtc aaagcccccg gcaaaagcac aggtagttgg
     1141 gtggccaccg gtgagaaact atagaaaaaa catgatgact cagcaaaaga cttcatccgg
     1201 tgctgaggag gcgagcagcg agaaggccgg gaatttcggt ggaggtgccg caggggcagg
     1261 cttggtaaaa gtctctatgg acggagcacc atcaggggat aacgcaggaa agaacatgtg
     1321 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     1381 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     1441 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     1501 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1561 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     1621 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     1681 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     1741 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     1801 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     1861 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     1921 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caa
//
