LOCUS       sequence-469333-        2256 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; type3B; NS3 systems; NS3a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pWN079
FEATURES             Location/Qualifiers
     source          1..2256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(621..655)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(726..1385)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1300..1319
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1567..2155
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2056..2075
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat tctaagaaaa aaggctcagt cgttatcgtt ggtcgtataa atttaagcgg
       61 cgatactgct tacgcccagc aaactagggg tgaagagggg tgtcaagaaa ccagccaaac
      121 tgggcgtgat aaaaatcaag tggaagggga ggtccagatt gtgtccacgg ctactcagac
      181 ctttttagcg actagcatta acggggtgct ttggactgtt taccacggcg cgggtacaag
      241 gacgatcgct tctcccaagg gcccagtgac tcaaatgtat accaacgtag acaaggatct
      301 ggttgggtgg caagccccac aaggatctcg tagcttgacg ccatgcacat gcgggtcctc
      361 agacctgtat ctggtaacaa gacacgcgga tgtaattccg gtcaggcgta ggggtgattc
      421 caggggtagc ctgttatctc cccgtccgat aagttactta aagggatcag cgggcggacc
      481 tttgctttgt ccggctgggc atgcagtagg catcttcagg gcggcggtca gcactagggg
      541 cgtagctaag gcagtagact tcatcccggt tgaaagtctt gaaacaacca tgagaagtcc
      601 aggatcctga gaccagacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
      661 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata
      721 tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
      781 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
      841 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
      901 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga aacgaaaaac
      961 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     1021 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     1081 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     1141 agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga
     1201 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     1261 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     1321 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     1381 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
     1441 gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa
     1501 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1561 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1621 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1681 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     1741 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1801 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1861 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1921 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     1981 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2041 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2101 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2161 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     2221 tcctgcgtta tcccctgatt ctgtggataa ccgtag
//
