LOCUS       sequence-469334-        1906 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; type2A; ABA systems; phlfO7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pRO232
FEATURES             Location/Qualifiers
     source          1..1906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..25
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(477..511)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(582..1241)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1156..1175
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(1423..1906,1..105)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
       61 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
      121 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
      181 tcccctgatt ctgtggataa ccgtagtcgg tctcaaacgg gatgatacga aacgtaccgt
      241 atcgttaagg tgaatgatac gaaacgtacc gtatcgttaa ggtcaatgat acgaaacgta
      301 ccgtatcgtt aaggtcaatg atacgaaacg taccgtatcg ttaaggtgta tgatacgaaa
      361 cgtaccgtat cgttaaggta gatgatacga aacgtaccgt atcgttaagg tcgatgatac
      421 gaaacgtacc gtatcgttaa ggtcggatct cagctagctt tgatgagacc agaccaataa
      481 aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta
      541 ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac
      601 tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg
      661 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg
      721 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg
      781 gtgaaactca cccagggatt ggctgaaacg aaaaacatat tctcaataaa ccctttaggg
      841 aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc
      901 cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa
      961 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata
     1021 cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac
     1081 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg
     1141 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg
     1201 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct
     1261 gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag
     1321 ttggaacctc ttacgtgccc gatcaatcat gaccaaaatc ccttaacgtg agttttcgtt
     1381 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     1441 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     1501 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     1561 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     1621 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     1681 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     1741 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     1801 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     1861 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagctt
//
