LOCUS       sequence-469335-        2010 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; type3B; Auxin systems; AID-Delta-34.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pWN022
FEATURES             Location/Qualifiers
     source          1..2010
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             218..349
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     terminator      complement(375..409)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(480..1139)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1054..1073
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1321..1909
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1810..1829
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat tctggatcag tggaactaaa cttgcgtgaa acggagcttt gtttgggcct
       61 gccaggaggg gacacagtag ctcctgtgac agggaataaa aggggtttct ctgaaacggt
      121 ggatctaaag ctaaatctaa acaatgaacc ggcgaataag gagggttcta ctactcacga
      181 cgttgtaaca tttgatagta aggagaagag cgcctgtcct aaggaccccg ccaaaccccc
      241 agcgaaggcg caagtcgtag gatggcctcc ggtaagaagc taccgtaaga atgtaatggt
      301 aagctgtcaa aaatcatccg ggggaccgga ggccgcggcc ttcgttaaag tatcaggatc
      361 ctgagaccag accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
      421 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat
      481 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
      541 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
      601 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
      661 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgaaacgaa aaacatattc
      721 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
      781 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
      841 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
      901 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
      961 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
     1021 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
     1081 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     1141 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     1201 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga ccaaaatccc
     1261 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     1321 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     1381 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     1441 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     1501 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     1561 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     1621 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     1681 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     1741 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     1801 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     1861 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     1921 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     1981 gttatcccct gattctgtgg ataaccgtag
//
