LOCUS       sequence-469339-        2556 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; type3B; NS3 systems; NS3-V2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pWN018
FEATURES             Location/Qualifiers
     source          1..2556
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1565..1591
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      complement(1611..1645)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1716..2375)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2290..2309
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtag tcggtctcat tctggggcga gcgcgggcat
      721 ggcgtcaatg actgggggcc agcaaatggg gagtacgttg taccaagagt ttgacgagat
      781 ggaagagtgt tcacaacatg gcaagaagaa gggtgacatc gacacgtaca gatacatagg
      841 ctctagtggg acaggctgcg tagtaatcgt gggcagaatt gtcctaagcg gatccggaac
      901 cagtgcgcct atcaccgcct acgcacaaca aaccagggga ctattgggct gtatcataac
      961 aagcctaact ggaagagaca aaaatcaagt cgagggcgag gttcagatcg tatctacggc
     1021 aactcagacc tttcttgcca cgtgcataaa cggcgtgtgc tgggccgtat atcatggggc
     1081 tggcacaaga acgatagcga gtcctaaggg tcctgttatt caaatgtata ccaacgtaga
     1141 ccaggatcta gtcggctggc ccgcgcccca aggctctagg agtcttaccc cttgcacgtg
     1201 cgggtcctca gacttatacc tggtaacgag gcacgcagat gttatacctg tgcgtagaag
     1261 gggtgacagt agaggatctc ttttatcccc ccgtcccatt agctacttaa agggtagctc
     1321 tggggggccg ttgttatgtc cggcaggaca tgccgtcggg ttattcagag cggctgtttg
     1381 tacgaggggt gttgcaaaag cagtagattt tataccggtt gagaacttag agacaacgat
     1441 gaggtccccg gtttttacag acaactcatc tccacctgct gttacactga cccaccccat
     1501 caccaaaatt gacagggagg ttctctacca ggaatttgac gaaatggagg agtgctcaca
     1561 acactatcca tacgacgtac cagattatgc tggatcctga gaccagacca ataaaaaacg
     1621 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
     1681 ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg
     1741 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga
     1801 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg
     1861 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
     1921 ctcacccagg gattggctga aacgaaaaac atattctcaa taaacccttt agggaaatag
     1981 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
     2041 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
     2101 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat
     2161 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
     2221 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
     2281 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
     2341 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
     2401 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
     2461 cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     2521 agcgtcagac cccgtagaaa agatcaaagg atcttc
//
