LOCUS       sequence-469341-        2568 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; type3B; ABA systems; ABI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pRO175
FEATURES             Location/Qualifiers
     source          1..2568
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(933..967)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1038..1697)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1612..1631
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1879..2467
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2368..2387
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat tctacgagag ttcctttgta tggcttcacc tcaatttgcg gtagaagacc
       61 cgagatggaa gccgccgtat cgacgatacc acgtttttta caatcctcct ccggctcaat
      121 gttagacgga cgttttgatc cccagagtgc agctcatttc tttggtgttt atgatggtca
      181 cgggggtagc caggtagcca actattgccg tgaaaggatg catttagcgt tagctgagga
      241 aatcgccaaa gaaaagccta tgttgtgtga tggagacacc tggttggaga agtggaaaaa
      301 ggcgttattc aacagcttcc taagggtgga tagtgagata gagtctgttg ctccggagac
      361 tgttggctca acatccgtgg tggccgtggt cttcccgagc catatttttg tggctaattg
      421 tggtgactct cgtgcggtct tgtgcagggg gaagactgca ttacctttaa gcgtcgatca
      481 taagccggac agggaggacg aagcagcgag gatagaggcc gccggtggaa aggtaataca
      541 atggaatggt gctagggtat tcggcgtact ggcaatgagt agatccattg gtgatagata
      601 cttgaagccg agcataatcc ctgaccctga agtaacagcg gttaaaaggg tgaaggaaga
      661 cgattgcctt atcttggctt ctgacggagt gtgggatgtc atgactgacg aggaagcctg
      721 cgaaatggca aggaaaagaa tactactttg gcataagaaa aatgcggtag ctggggacgc
      781 aagtctgtta gcagatgaaa gaagaaaaga aggcaaggac cccgccgcaa tgtcagccgc
      841 ggagtacctg tctaaacttg ctattcagag ggggagtaaa gataacatat ccgtggtcgt
      901 ggttgatcta aagggatcct gagaccagac caataaaaaa cgcccggcgg caaccgagcg
      961 ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa
     1021 gcgagctcga tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca
     1081 ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc
     1141 ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag
     1201 aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca gggattggct
     1261 gaaacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa
     1321 cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc
     1381 cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta
     1441 tcccatatca ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc
     1501 aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc
     1561 tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac
     1621 tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca
     1681 gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat
     1741 acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgcccgatc
     1801 aatcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     1861 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     1921 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     1981 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     2041 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     2101 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     2161 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     2221 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     2281 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     2341 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     2401 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     2461 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     2521 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtag
//
