LOCUS       sequence-469344-        2409 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; type3; rtTA systems; rtTA-v16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pRO217
FEATURES             Location/Qualifiers
     source          1..2409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     133..152
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1107..1141)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1212..1871)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1786..1805
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(2053..2409,1..232)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
       61 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      121 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      181 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      241 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      301 tgcgttatcc cctgattctg tggataaccg tagtcggtct catatgagtc gtcttgataa
      361 gtccaagatc ataaatagcg cactggaatt gctgaacggc gttggtattg agggtcttac
      421 cacgagaaag ctggcacaga agttgggggt cgaacaaccg accttgtact ggcatgtcaa
      481 gaataagcgt gcgctattag atgccttacc tatcgagatg ctagacagac atcatactca
      541 ctcatgtcca ttggaggggg agtcttggca agactttctt agaaacaatg caaagtcata
      601 taggtgcgcg ctactatcac atagagatgg ggccaaagtc catttgggca ccaggccgac
      661 cgaaaagcag tacgagactt tagagaacca gctggcattc ctgtgtcaac aaggcttcag
      721 tctagagaat gctctgtatg ctttaagcgc ggtcggccac ttcacgctag gttgtgtatt
      781 agaggaacaa gaacaccaag tggcaaaaga ggaaagagaa actccaacga ccgacagcat
      841 gccaccactg ttgaaacaag caatcgaact gtttgatcgt caaggggctg aaccagcatt
      901 cctattcggt ttggaattaa taatctgcgg tctggagaaa caattgaagt gcgaaagcgg
      961 cgggccgaca gatgccttag atgactttga cctagatatg ttaccagccg acgcccttga
     1021 cgactttgat ctggacatgc tgccggctga cgccttggat gacttcgact tggatatgtt
     1081 gccaggggga tcctgagacc agaccaataa aaaacgcccg gcggcaaccg agcgttctga
     1141 acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc
     1201 tcgatatcaa attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc
     1261 attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc
     1321 agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg
     1381 tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgaaacg
     1441 aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc
     1501 acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc
     1561 gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat
     1621 atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg
     1681 gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa
     1741 aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat
     1801 gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt
     1861 tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc
     1921 ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaatcat
     1981 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     2041 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2101 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2161 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     2221 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2281 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2341 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2401 ggagcgaac
//
