LOCUS       sequence-469345-        3545 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; ConL1/RE cassette; NS3 systems; 
            lexAO6-pLEU2m-mNeonGreen-tENO2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pAL001
FEATURES             Location/Qualifiers
     source          1..3545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     415..434
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2403..3194,3195..3263))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3026..3045
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3264..3368)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(3471..3545,1..514)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
       61 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
      121 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
      181 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
      241 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
      301 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
      361 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
      421 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
      481 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
      541 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
      601 tgtggataac cgtgcggccg cccctgaatt cgcatctaga acggggtcat cacggctcat
      661 catgcgccaa acaaatgtgt gcaatacacg ctcggatgac tgcatgatga ccgcactgac
      721 tggggacagc agatccacct aagcctgtga gagaagcaga cacccgacag atcaaggcag
      781 ttacgtctca ccaaaccaga tgtcaacaca gctacaacgg gtagtccatc gttgtaggat
      841 actgtatata cacccagtag agtaggtgac tactgtatga gcatacagta gtgacaacca
      901 cttactgtat ataaatacag tagtggtcat cgatactgta tataaaacca gtaggcaatc
      961 gtttgtactg tatgtacata cagtatacct cgcaactact gtatataaac acagtaatta
     1021 acttgtaata ttctaatcaa ttgacaatat tatttaagga cctattgttt tttccaatag
     1081 gtggttagca atcgtcttac tttctaactt ttcttacctt ttacatttca gcaatatata
     1141 tatatatatt tcaaggatat accattctaa gatctatggt ttcaaaaggg gaggaggata
     1201 atatggcgag tcttcctgcc acgcatgagc tgcatatctt cggatctatt aatggtgtcg
     1261 actttgatat ggtaggacaa ggaaccggga accccaatga cgggtatgaa gagctaaatc
     1321 taaagagtac aaaaggagat ttacagtttt ctccttggat acttgttccc cacattggat
     1381 atggatttca tcaatatctt ccttatccag acggaatgag cccgttccag gccgctatgg
     1441 ttgacggctc cgggtatcaa gtacaccgta ccatgcagtt tgaggatggg gcatccctga
     1501 ccgtaaatta caggtacacc tacgagggct cacacataaa gggtgaggcg caggtaaagg
     1561 gtactggatt tcctgcggac ggcccagtaa tgacaaattc acttacagca gcggattggt
     1621 gcaggagcaa aaagacctat cccaatgaca aaactatcat aagtaccttt aaatggtcct
     1681 atacgacagg taacgggaag agatataggt caacggcaag gacaacgtat actttcgcga
     1741 agccaatggc agcaaactat ctgaagaatc aacctatgta cgtgttcaga aagaccgagt
     1801 tgaaacattc caagacagag ttaaatttca aagaatggca aaaggctttt actgacgtaa
     1861 tggggatgga tgaattgtat aagggatcct aactcgagag tgcttttaac taagaattat
     1921 tagtcttttc tgcttatttt ttcatcatag tttagaacac tttatattaa cgaatagttt
     1981 atgaatctat ttaggtttaa aaattgatac agttttataa gttacttttt caaagactcg
     2041 tgctgtctat tgcataatgc actggaaggg gaaaaaaaag gtgcacacgc gtggcttttt
     2101 cttgaatttg cagtttgaaa aatgctgagc atgagacgga aatctgctcg tcagtggtgc
     2161 tcacactgac gaatcatgta cagatcatac cgatgactgc ctggcgactc acaactaagc
     2221 aagacagccg gaaccagcgc cggcgaacac cactgcatat atggcatatc acaacagtcc
     2281 aactagtgca ctgcagtaca gcggccgcga ttatcaaaaa ggatcttcac ctagatcctt
     2341 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2401 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     2461 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     2521 cccagtgctg caatgatacc gcgggaccca cgctcaccgg ctccagattt atcagcaata
     2581 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     2641 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     2701 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     2761 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     2821 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     2881 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     2941 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3001 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     3061 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3121 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     3181 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     3241 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     3301 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3361 gttccgcgca catttccccg aaaagtgcca cctgtcatga ccaaaatccc ttaacgtgag
     3421 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3481 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3541 tgttt
//
