LOCUS       sequence-469348-        4394 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; ConL1/R2 cassette; ABA systems; 
            pTDH3-NLS-VP48-2xOaf1-ABI-tENO1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pRO280
FEATURES             Location/Qualifiers
     source          1..4394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(103..894,895..963))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     726..745
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(964..1068)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1171..1759
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1660..1679
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2078..2744
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     2614..2637
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             2754..2774
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gcggccgcga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
       61 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
      121 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
      181 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
      241 gcgggaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
      301 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
      361 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
      421 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
      481 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
      541 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
      601 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
      661 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
      721 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
      781 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
      841 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
      901 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
      961 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     1021 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     1081 aaaagtgcca cctgtcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1141 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1201 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1261 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1321 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1381 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1441 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1501 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1561 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1621 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     1681 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     1741 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     1801 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtgc
     1861 ggccgcccct gaattcgcat ctagaacggg gtcatcacgg ctcatcatgc gccaaacaaa
     1921 tgtgtgcaat acacgctcgg atgactgcat gatgaccgca ctgactgggg acagcagatc
     1981 cacctaagcc tgtgagagaa gcagacaccc gacagatcaa ggcagttacg tctcaccaaa
     2041 ccagatgtca acacagctac aacgcagttc gagtttatca ttatcaatac tgccatttca
     2101 aagaatacgt aaataattaa tagtagtgat tttcctaact ttatttagtc aaaaaattag
     2161 ccttttaatt ctgctgtaac ccgtacatgc ccaaaatagg gggcgggtta cacagaatat
     2221 ataacatcgt aggtgtctgg gtgaacagtt tattcctggc atccactaaa tataatggag
     2281 cccgcttttt aagctggcat ccagaaaaaa aaagaatccc agcaccaaaa tattgttttc
     2341 ttcaccaacc atcagttcat aggtccattc tcttagcgca actacagaga acaggggcac
     2401 aaacaggcaa aaaacgggca caacctcaat ggagtgatgc aacctgcctg gagtaaatga
     2461 tgacacaagg caattgaccc acgcatgtat ctatctcatt ttcttacacc ttctattacc
     2521 ttctgctctc tctgatttgg aaaaagctga aaaaaaaggt tgaaaccagt tccctgaaat
     2581 tattccccta cttgactaat aagtatataa agacggtagg tattgattgt aattctgtaa
     2641 atctatttct taaacttctt aaattctact tttatagtta gtcttttttt tagttttaaa
     2701 acaccaagaa cttagtttcg aataaacaca cataaacaaa caaaagatct atgcccaaga
     2761 agaagcgtaa agtgccggcg gatgctctag acgatttcga tctggatatg ctaccagccg
     2821 atgcacttga cgatttcgac ttagacatgc ttccggccga cgctctagat gacttcgact
     2881 tagatatgct cggttccggc gcgaacaata caccatttcc gggatatttt gggggccttg
     2941 atttattcga ctacgacttc cttttcggca acgactttgc tggctcaggt gcgaataata
     3001 ctccttttcc aggttatttt ggaggtttgg atttatttga ttatgacttt ttgtttggca
     3061 atgactttgc tggttctacg agagttcctt tgtatggctt cacctcaatt tgcggtagaa
     3121 gacccgagat ggaagccgcc gtatcgacga taccacgttt tttacaatcc tcctccggct
     3181 caatgttaga cggacgtttt gatccccaga gtgcagctca tttctttggt gtttatgatg
     3241 gtcacggggg tagccaggta gccaactatt gccgtgaaag gatgcattta gcgttagctg
     3301 aggaaatcgc caaagaaaag cctatgttgt gtgatggaga cacctggttg gagaagtgga
     3361 aaaaggcgtt attcaacagc ttcctaaggg tggatagtga gatagagtct gttgctccgg
     3421 agactgttgg ctcaacatcc gtggtggccg tggtcttccc gagccatatt tttgtggcta
     3481 attgtggtga ctctcgtgcg gtcttgtgca gggggaagac tgcattacct ttaagcgtcg
     3541 atcataagcc ggacagggag gacgaagcag cgaggataga ggccgccggt ggaaaggtaa
     3601 tacaatggaa tggtgctagg gtattcggcg tactggcaat gagtagatcc attggtgata
     3661 gatacttgaa gccgagcata atccctgacc ctgaagtaac agcggttaaa agggtgaagg
     3721 aagacgattg ccttatcttg gcttctgacg gagtgtggga tgtcatgact gacgaggaag
     3781 cctgcgaaat ggcaaggaaa agaatactac tttggcataa gaaaaatgcg gtagctgggg
     3841 acgcaagtct gttagcagat gaaagaagaa aagaaggcaa ggaccccgcc gcaatgtcag
     3901 ccgcggagta cctgtctaaa cttgctattc agagggggag taaagataac atatccgtgg
     3961 tcgtggttga tctaaaggga tcctaactcg agagcttttg attaagcctt ctagtccaaa
     4021 aaacacgttt ttttgtcatt tatttcattt tcttagaata gtttagttta ttcattttat
     4081 agtcacgaat gttttatgat tctatatagg gttgcaaaca agcatttttc attttatgtt
     4141 aaaacaattt caggtttacc ttttattctg cttgtggtga cgcgtgtatc cgcccgctct
     4201 tttggtcacc catgtatgct ggatgtgaga cgataaatcg cagccaagtg agtgaataga
     4261 tgacgcacca cggtcagaca cggccacatc gtatctcaca ggagcaagcg cgataggagc
     4321 actcacacat agtacggtga tccgctgact cctttgccca aataagacgt gagccactag
     4381 tgcactgcag taca
//
