LOCUS       sequence-469350-        3411 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; type3B; Auxin systems; TIR1-U2 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pWN083
FEATURES             Location/Qualifiers
     source          1..3411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(2466..2500)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2571..3230)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3145..3164
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtag tcggtctcat tcttctggag gaggatctac
      721 gcgtacatat ttcccggaaa aggtagtaaa gcatatattg tcttttttac ccgctcaaag
      781 ggatcgtaat acggtttcac tggtatgcaa agtttggtat gagattgagc gtctttctag
      841 gaggggagtg tttgtgggca attgttatgc ggtccgtgcc ggccgtgtgg cagcaaggtt
      901 tccaaatgta agggccctga cggtgaaagg aaaaccgcat ggagctgact ttaacctggt
      961 cccaccggat tgggggggtt acgcgggtcc ttggatagaa gccgctgctc gtggatgcca
     1021 cgggctagaa gaactaagga tgaaaaggat ggtagtatcc gatgaatcat tggagctact
     1081 agctagaagc ttcccgcgtt tcagggcgtt ggtccttatt tcttgtgagg gcttctcaac
     1141 agatggatta gctgccgtgg ctagccattg caaacttctg agagagcttg acttacaaga
     1201 gaacgaagta gaagaccgtg gccctcgttg gctttcttgc tttccagaca gctgcactag
     1261 cctggtatct ttgaactttg cgtgcattaa gggtgaagtc aacgccggga gccttgaacg
     1321 tttggtatca agatctccga atctgagaag cctgcgttta aaccgttctg tctcagtaga
     1381 tacgctggct aaaatattac ttagaacacc aaacttggag gatctgggaa ccggcaactt
     1441 gacagacgac tttcagacag aaagctactt caagctaact tctgcgttgg aaaaatgtaa
     1501 gatgttgagg agcctgagtg gtttttggga cgccagtcct gtatgcctgt cattcattta
     1561 tcctctgtgt gcgcagctaa cgggactaaa cctgtcatac gctccgactc tagacgcctc
     1621 tgatctgact aaaatgatct ctcgttgtgt gaagcttcaa cgtctatggg tacttgattg
     1681 tatcagtgac aaagggttac aagtcgtagc ctccagttgt aaagacttac aggaacttag
     1741 ggtctttcct agtgactttt atgtagccgg atacagcgca gttactgagg aaggtttagt
     1801 ggcggtgagc ctaggatgcc ccaaattgaa tagtctactg tacttctgcc atcaaatgac
     1861 caatgcagcc ttagttaccg tcgctaagaa ttgtcctaac ttcacccgtt ttcgtttatg
     1921 catattggag cctggcaaac cagacgttgt cacttcacag ccccttgacg aaggctttgg
     1981 ggccatagtt cgtgaatgta aggggttgca aaggctaagt atttcagggc ttttaaccga
     2041 taaagtgttc atgtatatcg gaaaatatgc taagcagctt gaaatgctga gcatcgcttt
     2101 tgcaggtgac agcgataaag gcatgatgca cgtaatgaat gggtgtaaga acctgaggaa
     2161 actggagatc cgtgacagcc cgtttgggga tgctgcgttg ttagggaatt ttgctaggta
     2221 cgaaacgatg aggtccctgt ggatgagcag ttgtaatgtc accctaaagg gatgccaagt
     2281 tctagctagc aaaatgccga tgcttaatgt tgaagtaata aacgaaagag atggttcaaa
     2341 cgaaatggaa gaaaatcacg gagatcttcc gaaggtagag aaattatacg tgtaccgtac
     2401 cactgcgggc gcgagggacg acgcgcctaa ttttgttaaa atactaggat cctgagacca
     2461 gaccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
     2521 aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg
     2581 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
     2641 tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
     2701 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
     2761 tcaaaactgg tgaaactcac ccagggattg gctgaaacga aaaacatatt ctcaataaac
     2821 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
     2881 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
     2941 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
     3001 attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
     3061 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga
     3121 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga
     3181 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
     3241 ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta
     3301 tggtgaaagt tggaacctct tacgtgcccg atcaatcatg accaaaatcc cttaacgtga
     3361 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt c
//
