LOCUS       sequence-469351-        1966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; type4A; Activation domains; VP48-2XOaf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pAL002
FEATURES             Location/Qualifiers
     source          1..1966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             472..1131
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(538..557)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1202..1236
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     rep_origin      complement(join(1668..1966,1..290))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1748..1767)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
       61 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      121 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      181 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      241 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      301 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      361 tcatgattga tcgggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta
      421 ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa aatggagaaa
      481 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
      541 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
      601 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
      661 gcccgcctga tgaatgctca tccggaattt cgtatggcaa tgaaagacgg tgagctggtg
      721 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
      781 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
      841 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
      901 ttcgtttcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
      961 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg
     1021 ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga
     1081 atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta atttgatatc
     1141 gagctcgctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc atctggattt
     1201 gttcagaacg ctcggttgcc gccgggcgtt ttttattggt ctggtctcaa tccccggcgg
     1261 atgctctaga cgatttcgat ctggatatgc taccagccga tgcacttgac gatttcgact
     1321 tagacatgct tccggccgac gctctagatg acttcgactt agatatgctc ggttccggcg
     1381 cgaacaatac accatttccg ggatattttg ggggccttga tttattcgac tacgacttcc
     1441 ttttcggcaa cgactttgct ggctcaggtg cgaataatac tccttttcca ggttattttg
     1501 gaggtttgga tttatttgat tatgactttt tgtttggcaa tgactttgct taatggctga
     1561 gaccgactac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1621 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1681 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1741 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1801 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1861 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1921 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggta
//
