LOCUS       sequence-469352-        1984 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; type4A; Activation domains; NLS-VP48-2XOaf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pWN102
FEATURES             Location/Qualifiers
     source          1..1984
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             14..34
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      complement(349..383)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(454..1113)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1028..1047
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1295..1883
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1784..1803
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa tcccccaaga agaagcgtaa agtgccggcg gatgctctag acgatttcga
       61 tctggatatg ctaccagccg atgcacttga cgatttcgac ttagacatgc ttccggccga
      121 cgctctagat gacttcgact tagatatgct cggttccggc gcgaacaata caccatttcc
      181 gggatatttt gggggccttg atttattcga ctacgacttc cttttcggca acgactttgc
      241 tggctcaggt gcgaataata ctccttttcc aggttatttt ggaggtttgg atttatttga
      301 ttatgacttt ttgtttggca atgactttgc ttggctgaga ccagaccaat aaaaaacgcc
      361 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
      421 atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca
      481 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
      541 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
      601 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
      661 cacccaggga ttggctgaaa cgaaaaacat attctcaata aaccctttag ggaaataggc
      721 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
      781 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
      841 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc
      901 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
      961 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
     1021 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
     1081 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
     1141 cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc
     1201 tcttacgtgc ccgatcaatc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1261 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1321 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1381 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1441 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1501 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     1561 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     1621 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     1681 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     1741 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     1801 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     1861 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     1921 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     1981 gtag
//
