LOCUS       sequence-469353-        2409 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; type3; rtTA systems; rtTA-v10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pRO112
FEATURES             Location/Qualifiers
     source          1..2409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     156..175
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             367..1110
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     terminator      complement(1130..1164)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1235..1894)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1809..1828
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(2076..2409,1..255)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
       61 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
      121 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
      181 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
      241 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
      301 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtagtcgg
      361 tctcatatgt cccgtcttga taagagtaag gttatcaact cagccctaga gctgctgaac
      421 ggcgtcggaa tcgaggggct tactaccaga aaactagcac agaaactggg tgtagagcag
      481 cccacgcttt actggcatgt taaaaataag agagcactat tagacgcgtt gccgatcgaa
      541 atgttggata ggcatcacac acactcatgc cctttagaag gagaaagttg gcaggatttt
      601 ctgagaaata atgcgaagtc ttatcgttgt gcgttacttt cccacaggga cggtgctaaa
      661 gtacatttgg gaactagacc aactgaaaaa caatatgaga cattggagaa ccaactggcc
      721 ttcttatgcc aacagggttt cagtcttgag aacgcccttt acgccctttc agcggttggg
      781 cacttcactc ttggttgcgt cttggaagag caggagcatc aagtagcaaa agaggagcgt
      841 gaaaccccca caacagactc catgccccct ctattgaagc aagctatcga attattcgac
      901 cgtcaaggag cggaaccagc atttttattt ggcttagaat tgattatttg tgggttagaa
      961 aaacaactaa agtgtgagag cggtggaccg acagacgctc ttgatgattt tgacttggat
     1021 atgttacccg ccgacgcttt ggacgacttc gatttggata tgctgcctgc ggatgcacta
     1081 gatgatttcg atctggacat gttaccgggt ggatcctgag accagaccaa taaaaaacgc
     1141 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
     1201 tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc
     1261 agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
     1321 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
     1381 tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac
     1441 tcacccaggg attggctgaa acgaaaaaca tattctcaat aaacccttta gggaaatagg
     1501 ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat
     1561 cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt
     1621 aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt
     1681 ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct
     1741 tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg
     1801 tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat
     1861 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
     1921 tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac
     1981 ctcttacgtg cccgatcaat catgaccaaa atcccttaac gtgagttttc gttccactga
     2041 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2101 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2161 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2221 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2281 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2341 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     2401 ggttcgtgc
//
