LOCUS       sequence-469354-        1848 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; type2A; Auxin systems; amtRO7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pRO286
FEATURES             Location/Qualifiers
     source          1..1848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     402..421
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(815..849)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(920..1579)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1494..1513
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(1761..1848,1..501)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
       61 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
      121 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
      181 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
      241 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
      301 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
      361 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
      421 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
      481 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
      541 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
      601 agtcggtctc aaacgggttc tatcgatcta tagataatga ttctatcgat ctatagataa
      661 tcattctatc gatctataga taatcattct atcgatctat agataatgtt tctatcgatc
      721 tatagataat agttctatcg atctatagat aatcgttcta tcgatctata gataatcgga
      781 tctcagctag ctagatcttg atgagaccag accaataaaa aacgcccggc ggcaaccgag
      841 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
      901 aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt
      961 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca
     1021 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
     1081 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
     1141 ctgaaacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
     1201 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
     1261 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
     1321 tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca
     1381 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
     1441 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
     1501 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
     1561 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa
     1621 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga
     1681 tcaatcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1741 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1801 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagag
//
