LOCUS       sequence-469355-        3895 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; ConLS/RE cassette; Const. mNG;  pRNR2-mNeonGreen-tENO2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pRO262
FEATURES             Location/Qualifiers
     source          1..3895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(2140..2931,2932..3000))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2763..2782
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3001..3105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3208..3796
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3697..3716
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcggccgccc ctgaattcgc atctagatgg tagagccaca aacagccggt acaagcaacg
       61 atctccagga ccatctgaat catgcgcgga tgacacgaac tcacgacggc gatcacagac
      121 attaacccac agtacagaca ctgcgacaac gtggcaattc gtcgcaatac cgtctcactg
      181 aactggccga taattgcaga cgaacgagtc gaacaagaag caggcaaagt ttagagcact
      241 gcccctccgc actcaaaaaa gaaaaaacta ggaggaaaat aaaattctca accacacaaa
      301 cacataaaca catacaaata caaatacaag cttatttact tgacatcgcg cgatcttcca
      361 ctattcagcg ccgtccgccc tctctcgtgt tttttgttta cgcgacaact atgcgaaatc
      421 cggagcaacg ggcaaccgtt tggggaaaga ccacacccac gcgcgatcgc catggcaacg
      481 aggtcgcaca cgccccacac ccagacctcc ctgcgagcgg gcatgggtac aatgtccccg
      541 ttgccacaga caccacttcg tagcacagcg cagagcgtag cgtgttgttg ctgctgacaa
      601 aagaaaattt ttcttagcaa agcaaaggag gggaagcacg ggcagatagc accgtaccat
      661 acccttggaa actcgaaatg aacgaagcag gaaatgagag aatgagagtt ttgtaggtat
      721 atatagcggt agtgtttgcg cgttaccatc atcttctgga tctatctatt gttcttttcc
      781 tcatcacttt cccctttttc gctcttcttc ttgtctttta tttctttctt ttttttaatt
      841 gttccctcga ttggctatct accaaagaat ccaaacttaa tacacgtatt tatttgtcca
      901 attaccagat ctatggtttc aaaaggggag gaggataata tggcgagtct tcctgccacg
      961 catgagctgc atatcttcgg atctattaat ggtgtcgact ttgatatggt aggacaagga
     1021 accgggaacc ccaatgacgg gtatgaagag ctaaatctaa agagtacaaa aggagattta
     1081 cagttttctc cttggatact tgttccccac attggatatg gatttcatca atatcttcct
     1141 tatccagacg gaatgagccc gttccaggcc gctatggttg acggctccgg gtatcaagta
     1201 caccgtacca tgcagtttga ggatggggca tccctgaccg taaattacag gtacacctac
     1261 gagggctcac acataaaggg tgaggcgcag gtaaagggta ctggatttcc tgcggacggc
     1321 ccagtaatga caaattcact tacagcagcg gattggtgca ggagcaaaaa gacctatccc
     1381 aatgacaaaa ctatcataag tacctttaaa tggtcctata cgacaggtaa cgggaagaga
     1441 tataggtcaa cggcaaggac aacgtatact ttcgcgaagc caatggcagc aaactatctg
     1501 aagaatcaac ctatgtacgt gttcagaaag accgagttga aacattccaa gacagagtta
     1561 aatttcaaag aatggcaaaa ggcttttact gacgtaatgg ggatggatga attgtataag
     1621 ggatcctaac tcgagagtgc ttttaactaa gaattattag tcttttctgc ttattttttc
     1681 atcatagttt agaacacttt atattaacga atagtttatg aatctattta ggtttaaaaa
     1741 ttgatacagt tttataagtt actttttcaa agactcgtgc tgtctattgc ataatgcact
     1801 ggaaggggaa aaaaaaggtg cacacgcgtg gctttttctt gaatttgcag tttgaaaaat
     1861 gctgagcatg agacggaaat ctgctcgtca gtggtgctca cactgacgaa tcatgtacag
     1921 atcataccga tgactgcctg gcgactcaca actaagcaag acagccggaa ccagcgccgg
     1981 cgaacaccac tgcatatatg gcatatcaca acagtccaac tagtgcactg cagtacagcg
     2041 gccgcgatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2101 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     2161 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     2221 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     2281 ggacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     2341 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     2401 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     2461 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     2521 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     2581 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     2641 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     2701 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     2761 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     2821 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     2881 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     2941 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3001 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3061 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     3121 agtgccacct gtcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     3181 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3241 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3301 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     3361 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3421 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3481 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3541 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3601 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     3661 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     3721 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     3781 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     3841 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgt
//
