LOCUS       sequence-469356-        3506 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; ConL2/RE cassette; Auxin systems; 
            amtRO7-pLEU2m-mNeonGreen-tENO2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pWN020
FEATURES             Location/Qualifiers
     source          1..3506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(103..894,895..963))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     726..745
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(964..1068)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1171..1759
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1660..1679
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcggccgcga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
       61 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
      121 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
      181 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
      241 gcgggaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
      301 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
      361 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
      421 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
      481 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
      541 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
      601 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
      661 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
      721 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
      781 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
      841 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
      901 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
      961 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     1021 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     1081 aaaagtgcca cctgtcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1141 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1201 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1261 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1321 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1381 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1441 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1501 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1561 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1621 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     1681 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     1741 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     1801 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtgc
     1861 ggccgcccct gaattcgcat ctagaataaa tcgcagccaa gtgagtgaat agatgacgca
     1921 ccacggtcag acacggccac atcgtatctc acaggagcaa gcgcgatagg agcactcaca
     1981 catagtacgg tgatccgctg actcctttgc ccaaataaga cgtgagcccg tctcagatgc
     2041 acacactggc ttaagatgac aacgggttct atcgatctat agataatgat tctatcgatc
     2101 tatagataat cattctatcg atctatagat aatcattcta tcgatctata gataatgttt
     2161 ctatcgatct atagataata gttctatcga tctatagata atcgttctat cgatctatag
     2221 ataatcggat ctcagctagc tagatcttga caatattatt taaggaccta ttgttttttc
     2281 caataggtgg ttagcaatcg tcttactttc taacttttct taccttttac atttcagcaa
     2341 tatatatata tatatttcaa ggatatacca ttctaagatc tatggtttca aaaggggagg
     2401 aggataatat ggcgagtctt cctgccacgc atgagctgca tatcttcgga tctattaatg
     2461 gtgtcgactt tgatatggta ggacaaggaa ccgggaaccc caatgacggg tatgaagagc
     2521 taaatctaaa gagtacaaaa ggagatttac agttttctcc ttggatactt gttccccaca
     2581 ttggatatgg atttcatcaa tatcttcctt atccagacgg aatgagcccg ttccaggccg
     2641 ctatggttga cggctccggg tatcaagtac accgtaccat gcagtttgag gatggggcat
     2701 ccctgaccgt aaattacagg tacacctacg agggctcaca cataaagggt gaggcgcagg
     2761 taaagggtac tggatttcct gcggacggcc cagtaatgac aaattcactt acagcagcgg
     2821 attggtgcag gagcaaaaag acctatccca atgacaaaac tatcataagt acctttaaat
     2881 ggtcctatac gacaggtaac gggaagagat ataggtcaac ggcaaggaca acgtatactt
     2941 tcgcgaagcc aatggcagca aactatctga agaatcaacc tatgtacgtg ttcagaaaga
     3001 ccgagttgaa acattccaag acagagttaa atttcaaaga atggcaaaag gcttttactg
     3061 acgtaatggg gatggatgaa ttgtataagg gatcctaact cgagagtgct tttaactaag
     3121 aattattagt cttttctgct tattttttca tcatagttta gaacacttta tattaacgaa
     3181 tagtttatga atctatttag gtttaaaaat tgatacagtt ttataagtta ctttttcaaa
     3241 gactcgtgct gtctattgca taatgcactg gaaggggaaa aaaaaggtgc acacgcgtgg
     3301 ctttttcttg aatttgcagt ttgaaaaatg ctgagcatga gacggaaatc tgctcgtcag
     3361 tggtgctcac actgacgaat catgtacaga tcataccgat gactgcctgg cgactcacaa
     3421 ctaagcaaga cagccggaac cagcgccggc gaacaccact gcatatatgg catatcacaa
     3481 cagtccaact agtgcactgc agtaca
//
