LOCUS       sequence-469358-        2373 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; type3; Reporter; mNeonGreen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pRO117
FEATURES             Location/Qualifiers
     source          1..2373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(738..772)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(843..1502)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1417..1436
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1684..2272
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2173..2192
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat atggtttcaa aaggggagga ggataatatg gcgagtcttc ctgccacgca
       61 tgagctgcat atcttcggat ctattaatgg tgtcgacttt gatatggtag gacaaggaac
      121 cgggaacccc aatgacgggt atgaagagct aaatctaaag agtacaaaag gagatttaca
      181 gttttctcct tggatacttg ttccccacat tggatatgga tttcatcaat atcttcctta
      241 tccagacgga atgagcccgt tccaggccgc tatggttgac ggctccgggt atcaagtaca
      301 ccgtaccatg cagtttgagg atggggcatc cctgaccgta aattacaggt acacctacga
      361 gggctcacac ataaagggtg aggcgcaggt aaagggtact ggatttcctg cggacggccc
      421 agtaatgaca aattcactta cagcagcgga ttggtgcagg agcaaaaaga cctatcccaa
      481 tgacaaaact atcataagta cctttaaatg gtcctatacg acaggtaacg ggaagagata
      541 taggtcaacg gcaaggacaa cgtatacttt cgcgaagcca atggcagcaa actatctgaa
      601 gaatcaacct atgtacgtgt tcagaaagac cgagttgaaa cattccaaga cagagttaaa
      661 tttcaaagaa tggcaaaagg cttttactga cgtaatgggg atggatgaat tgtataaggg
      721 atcctgagac cagaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca
      781 gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca
      841 aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg
      901 acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg
      961 tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg
     1021 gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgaaac gaaaaacata
     1081 ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc
     1141 gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac
     1201 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc
     1261 tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg
     1321 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
     1381 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
     1441 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc
     1501 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
     1561 cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaatca tgaccaaaat
     1621 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     1681 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     1741 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1801 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     1861 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1921 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1981 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     2041 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     2101 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     2161 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     2221 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     2281 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     2341 tgcgttatcc cctgattctg tggataaccg tag
//
