LOCUS       sequence-469359-        2283 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; type3B; NS3 systems; Linker-NS3a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pWN080
FEATURES             Location/Qualifiers
     source          1..2283
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(648..682)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(753..1412)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1327..1346
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1594..2182
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2083..2102
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat tctggaggtg gcagcgccgg ctctggaggc aagaagaagg gaagcgttgt
       61 tatcgtcgga aggattaacc tgagcggtga tacggcttac gcacagcaga ctagaggcga
      121 agaggggtgc caagagacat ctcaaacagg tagggacaaa aatcaagttg aaggcgaggt
      181 gcaaatcgtg tcaacggcga cgcaaacatt ccttgctaca agcataaacg gcgttctgtg
      241 gaccgtatac cacggtgctg gcacaagaac catagcaagt cccaaagggc cagtcacaca
      301 gatgtacact aatgtagata aggaccttgt cggctggcag gcgcctcagg gaagcagaag
      361 tttaacacca tgcacatgtg gctccagcga cctatatctt gttacacgtc atgctgatgt
      421 gataccagta cgtagaaggg gggactcaag gggatctttg ctttcaccac gtccaatctc
      481 atacttgaag ggtagcgcag gtggccccct tttatgtccc gcaggacatg cggttgggat
      541 ctttcgtgca gcagtttcaa ctagaggtgt tgcgaaagct gttgatttta ttcctgttga
      601 gtcattagaa accactatga ggagtccagg atcctgagac cagaccaata aaaaacgccc
      661 ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta
      721 tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag
      781 tactgttgta attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga
      841 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg
      901 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc
      961 acccagggat tggctgaaac gaaaaacata ttctcaataa accctttagg gaaataggcc
     1021 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg
     1081 tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa
     1141 caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc
     1201 ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta
     1261 tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta
     1321 cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca
     1381 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
     1441 gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct
     1501 cttacgtgcc cgatcaatca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1561 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1621 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     1681 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     1741 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     1801 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     1861 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     1921 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     1981 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     2041 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     2101 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     2161 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     2221 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     2281 tag
//
