LOCUS       sequence-469360-        2313 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; type3A; NS3 systems; LexA-NES.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pWN062
FEATURES             Location/Qualifiers
     source          1..2313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(11..262,263..616)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     CDS             632..658
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     terminator      complement(678..712)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(783..1442)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1357..1376
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1624..2212
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2113..2132
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat atgaaggcac taacggcgcg tcaacaggaa gtatttgatc taatcagaga
       61 tcacatttct cagacgggca tgccacctac gagagcggaa attgcgcaga ggctggggtt
      121 tcgttcacca aacgcggccg aggaacacct gaaggctcta gcaagaaaag gtgtcattga
      181 gatagtctcc ggtgcctcca ggggaatcag gcttctacaa gaggaggagg aaggactgcc
      241 acttgtagga agggtcgctg caggggagcc tctattggcg cagcagcata tcgaaggtca
      301 ttatcaggtc gatccatctt tgttcaagcc caatgccgat ttcttacttc gtgtgagtgg
      361 aatgagtatg aaagatatag gcattatgga tggggatcta cttgctgttc ataaaacaca
      421 agatgtgaga aatggtcagg tagttgtagc aaggatcgat gacgaagtca cagttaaaag
      481 gctaaagaag caaggcaata aagtagagtt attacccgag aactcagaat ttaagcctat
      541 tgtggtcgac ctacgtcaac agagcttcac catagaaggt ctggccgtcg gtgttatccg
      601 taacggtgat tggctggggt cagggtcacc tctgcctccg ttagaaagac taacgctggg
      661 ttcttgagac cagaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca
      721 gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca
      781 aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg
      841 acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg
      901 tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg
      961 gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgaaac gaaaaacata
     1021 ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc
     1081 gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac
     1141 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc
     1201 tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg
     1261 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
     1321 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
     1381 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc
     1441 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
     1501 cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaatca tgaccaaaat
     1561 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     1621 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     1681 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1741 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     1801 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1861 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1921 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     1981 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     2041 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     2101 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     2161 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     2221 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     2281 tgcgttatcc cctgattctg tggataaccg tag
//
