LOCUS       sequence-469361-        2214 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; type3B; ABA systems; PYL1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pRO177
FEATURES             Location/Qualifiers
     source          1..2214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(579..613)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(684..1343)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1258..1277
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1525..2113
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2014..2033
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat tctggtggag gtgctccaac acaagatgag ttcacacaac tatcacagag
       61 tatcgcagaa ttccatacct accaactagg aaatggaaga tgttcttcac tgcttgcgca
      121 aagaatccat gccccgccgg agactgtgtg gagtgtagtt aggaggttcg acaggccgca
      181 gatatacaag cactttatca aatcctgcaa tgttagcgaa gatttcgaga tgcgtgttgg
      241 atgcaccagg gacgtaaatg ttatatctgg attacctgcg aatacgtcaa gagaaaggtt
      301 ggacttacta gacgacgaca gacgtgttac cggattttcc ataacgggtg gtgaacatcg
      361 tctacgtaat tataagagcg tgactacagt ccaccgtttt gaaaaggagg aagaggagga
      421 gagaatatgg accgtggttc tggaatccta cgttgttgat gtacccgagg ggaatagcga
      481 ggaggataca cgtctgttcg cggataccgt tatcagattg aatcttcaaa aacttgcttc
      541 cattacagag gccatgaacg gatcctgaga ccagaccaat aaaaaacgcc cggcggcaac
      601 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga
      661 gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt
      721 aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc
      781 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg
      841 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
      901 ttggctgaaa cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
      961 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
     1021 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
     1081 acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca
     1141 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
     1201 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
     1261 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
     1321 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca
     1381 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc
     1441 ccgatcaatc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1501 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1561 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1621 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     1681 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1741 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1801 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1861 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1921 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1981 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     2041 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     2101 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     2161 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtag
//
