LOCUS       sequence-469363-        4304 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; ConL1/R2 cassette; ABA systems; 
            pTDH3-NLS-VP48-1xOaf1-ABI-tENO1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pRO279
FEATURES             Location/Qualifiers
     source          1..4304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        220..886
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     756..779
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             896..916
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(2549..3340,3341..3409))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3172..3191
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3410..3514)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3617..4205
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4106..4125
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcggccgccc ctgaattcgc atctagaacg gggtcatcac ggctcatcat gcgccaaaca
       61 aatgtgtgca atacacgctc ggatgactgc atgatgaccg cactgactgg ggacagcaga
      121 tccacctaag cctgtgagag aagcagacac ccgacagatc aaggcagtta cgtctcacca
      181 aaccagatgt caacacagct acaacgcagt tcgagtttat cattatcaat actgccattt
      241 caaagaatac gtaaataatt aatagtagtg attttcctaa ctttatttag tcaaaaaatt
      301 agccttttaa ttctgctgta acccgtacat gcccaaaata gggggcgggt tacacagaat
      361 atataacatc gtaggtgtct gggtgaacag tttattcctg gcatccacta aatataatgg
      421 agcccgcttt ttaagctggc atccagaaaa aaaaagaatc ccagcaccaa aatattgttt
      481 tcttcaccaa ccatcagttc ataggtccat tctcttagcg caactacaga gaacaggggc
      541 acaaacaggc aaaaaacggg cacaacctca atggagtgat gcaacctgcc tggagtaaat
      601 gatgacacaa ggcaattgac ccacgcatgt atctatctca ttttcttaca ccttctatta
      661 ccttctgctc tctctgattt ggaaaaagct gaaaaaaaag gttgaaacca gttccctgaa
      721 attattcccc tacttgacta ataagtatat aaagacggta ggtattgatt gtaattctgt
      781 aaatctattt cttaaacttc ttaaattcta cttttatagt tagtcttttt tttagtttta
      841 aaacaccaag aacttagttt cgaataaaca cacataaaca aacaaaagat ctatgcccaa
      901 gaagaagcgt aaagtgccgg cggatgctct agacgatttc gatctggata tgctaccagc
      961 cgatgcactt gacgatttcg acttagacat gcttccggcc gacgctctag atgacttcga
     1021 cttagatatg ctcggctcag gtgcgaataa tactcctttt ccaggttatt ttggaggttt
     1081 ggatttattt gattatgact ttttgtttgg caatgacttt gctggttcta cgagagttcc
     1141 tttgtatggc ttcacctcaa tttgcggtag aagacccgag atggaagccg ccgtatcgac
     1201 gataccacgt tttttacaat cctcctccgg ctcaatgtta gacggacgtt ttgatcccca
     1261 gagtgcagct catttctttg gtgtttatga tggtcacggg ggtagccagg tagccaacta
     1321 ttgccgtgaa aggatgcatt tagcgttagc tgaggaaatc gccaaagaaa agcctatgtt
     1381 gtgtgatgga gacacctggt tggagaagtg gaaaaaggcg ttattcaaca gcttcctaag
     1441 ggtggatagt gagatagagt ctgttgctcc ggagactgtt ggctcaacat ccgtggtggc
     1501 cgtggtcttc ccgagccata tttttgtggc taattgtggt gactctcgtg cggtcttgtg
     1561 cagggggaag actgcattac ctttaagcgt cgatcataag ccggacaggg aggacgaagc
     1621 agcgaggata gaggccgccg gtggaaaggt aatacaatgg aatggtgcta gggtattcgg
     1681 cgtactggca atgagtagat ccattggtga tagatacttg aagccgagca taatccctga
     1741 ccctgaagta acagcggtta aaagggtgaa ggaagacgat tgccttatct tggcttctga
     1801 cggagtgtgg gatgtcatga ctgacgagga agcctgcgaa atggcaagga aaagaatact
     1861 actttggcat aagaaaaatg cggtagctgg ggacgcaagt ctgttagcag atgaaagaag
     1921 aaaagaaggc aaggaccccg ccgcaatgtc agccgcggag tacctgtcta aacttgctat
     1981 tcagaggggg agtaaagata acatatccgt ggtcgtggtt gatctaaagg gatcctaact
     2041 cgagagcttt tgattaagcc ttctagtcca aaaaacacgt ttttttgtca tttatttcat
     2101 tttcttagaa tagtttagtt tattcatttt atagtcacga atgttttatg attctatata
     2161 gggttgcaaa caagcatttt tcattttatg ttaaaacaat ttcaggttta ccttttattc
     2221 tgcttgtggt gacgcgtgta tccgcccgct cttttggtca cccatgtatg ctggatgtga
     2281 gacgataaat cgcagccaag tgagtgaata gatgacgcac cacggtcaga cacggccaca
     2341 tcgtatctca caggagcaag cgcgatagga gcactcacac atagtacggt gatccgctga
     2401 ctcctttgcc caaataagac gtgagccact agtgcactgc agtacagcgg ccgcgattat
     2461 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     2521 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     2581 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     2641 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcgg gacccacgct
     2701 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     2761 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     2821 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     2881 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     2941 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     3001 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     3061 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     3121 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     3181 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     3241 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     3301 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     3361 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     3421 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     3481 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     3541 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     3601 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3661 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3721 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     3781 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3841 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3901 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3961 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     4021 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4081 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4141 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4201 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4261 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgt
//
