LOCUS       sequence-469365-        5624 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; ConLS/R1 cassette; Auxin systems; 
            pTDH3-AmtR-NES-TIR1-U2-tADH1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 5624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pWN086
FEATURES             Location/Qualifiers
     source          1..5624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(103..894,895..963))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     726..745
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(964..1068)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1171..1759
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2078..2744
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     2614..2637
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             3432..3458
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     terminator      5223..5410
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5278..5302)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 gcggccgcga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
       61 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
      121 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
      181 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
      241 gcgggaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
      301 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
      361 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
      421 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
      481 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
      541 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
      601 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
      661 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
      721 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
      781 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
      841 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
      901 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
      961 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     1021 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     1081 aaaagtgcca cctgtcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1141 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1201 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1261 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1321 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1381 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1441 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1501 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1561 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1621 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     1681 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     1741 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     1801 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtgc
     1861 ggccgcccct gaattcgcat ctagatggta gagccacaaa cagccggtac aagcaacgat
     1921 ctccaggacc atctgaatca tgcgcggatg acacgaactc acgacggcga tcacagacat
     1981 taacccacag tacagacact gcgacaacgt ggcaattcgt cgcaataccg tctcactgaa
     2041 ctggccgata attgcagacg aacgcagttc gagtttatca ttatcaatac tgccatttca
     2101 aagaatacgt aaataattaa tagtagtgat tttcctaact ttatttagtc aaaaaattag
     2161 ccttttaatt ctgctgtaac ccgtacatgc ccaaaatagg gggcgggtta cacagaatat
     2221 ataacatcgt aggtgtctgg gtgaacagtt tattcctggc atccactaaa tataatggag
     2281 cccgcttttt aagctggcat ccagaaaaaa aaagaatccc agcaccaaaa tattgttttc
     2341 ttcaccaacc atcagttcat aggtccattc tcttagcgca actacagaga acaggggcac
     2401 aaacaggcaa aaaacgggca caacctcaat ggagtgatgc aacctgcctg gagtaaatga
     2461 tgacacaagg caattgaccc acgcatgtat ctatctcatt ttcttacacc ttctattacc
     2521 ttctgctctc tctgatttgg aaaaagctga aaaaaaaggt tgaaaccagt tccctgaaat
     2581 tattccccta cttgactaat aagtatataa agacggtagg tattgattgt aattctgtaa
     2641 atctatttct taaacttctt aaattctact tttatagtta gtcttttttt tagttttaaa
     2701 acaccaagaa cttagtttcg aataaacaca cataaacaaa caaaagatct atggctggtg
     2761 ctgtgggaag gccaaggagg tccgccccca ggagagcggg gaaaaatcct cgtgaagaga
     2821 tactggacgc atctgctgag ctttttacgc gtcaaggctt tgctactact tcaactcacc
     2881 agatagcaga tgcggtcggc attagacagg cttctctata ttatcatttt ccgtcaaaaa
     2941 cggagatttt ccttacacta cttaagtcta cagttgagcc ttcaactgtc ttggcagagg
     3001 atctatccac ccttgacgcc ggtcccgaga tgagattgtg ggctatcgta gcgagtgagg
     3061 tgcgtttatt gctatctaca aaatggaatg taggaaggct ataccaattg cctatcgtgg
     3121 gctcagagga gttcgctgaa taccattccc agagggaggc gcttacaaat gtctttcgtg
     3181 atcttgctac agaaattgta ggagatgacc ccagagcgga gctgcccttc cacataacta
     3241 tgagcgtgat agagatgcgt aggaacgatg gaaagattcc aagtccatta agtgcagata
     3301 gtcttccaga aaccgcaatc atgttggctg acgccagctt ggccgtcctg ggagcacccc
     3361 taccggccga tagggttgaa aagacgcttg aattgattaa acaggcagac gcaaaggcaa
     3421 gcggaagcgg actgcctccg ttagaaagac taacgctggg ttcttctgga ggaggatcta
     3481 cgcgtacata tttcccggaa aaggtagtaa agcatatatt gtctttttta cccgctcaaa
     3541 gggatcgtaa tacggtttca ctggtatgca aagtttggta tgagattgag cgtctttcta
     3601 ggaggggagt gtttgtgggc aattgttatg cggtccgtgc cggccgtgtg gcagcaaggt
     3661 ttccaaatgt aagggccctg acggtgaaag gaaaaccgca tggagctgac tttaacctgg
     3721 tcccaccgga ttgggggggt tacgcgggtc cttggataga agccgctgct cgtggatgcc
     3781 acgggctaga agaactaagg atgaaaagga tggtagtatc cgatgaatca ttggagctac
     3841 tagctagaag cttcccgcgt ttcagggcgt tggtccttat ttcttgtgag ggcttctcaa
     3901 cagatggatt agctgccgtg gctagccatt gcaaacttct gagagagctt gacttacaag
     3961 agaacgaagt agaagaccgt ggccctcgtt ggctttcttg ctttccagac agctgcacta
     4021 gcctggtatc tttgaacttt gcgtgcatta agggtgaagt caacgccggg agccttgaac
     4081 gtttggtatc aagatctccg aatctgagaa gcctgcgttt aaaccgttct gtctcagtag
     4141 atacgctggc taaaatatta cttagaacac caaacttgga ggatctggga accggcaact
     4201 tgacagacga ctttcagaca gaaagctact tcaagctaac ttctgcgttg gaaaaatgta
     4261 agatgttgag gagcctgagt ggtttttggg acgccagtcc tgtatgcctg tcattcattt
     4321 atcctctgtg tgcgcagcta acgggactaa acctgtcata cgctccgact ctagacgcct
     4381 ctgatctgac taaaatgatc tctcgttgtg tgaagcttca acgtctatgg gtacttgatt
     4441 gtatcagtga caaagggtta caagtcgtag cctccagttg taaagactta caggaactta
     4501 gggtctttcc tagtgacttt tatgtagccg gatacagcgc agttactgag gaaggtttag
     4561 tggcggtgag cctaggatgc cccaaattga atagtctact gtacttctgc catcaaatga
     4621 ccaatgcagc cttagttacc gtcgctaaga attgtcctaa cttcacccgt tttcgtttat
     4681 gcatattgga gcctggcaaa ccagacgttg tcacttcaca gccccttgac gaaggctttg
     4741 gggccatagt tcgtgaatgt aaggggttgc aaaggctaag tatttcaggg cttttaaccg
     4801 ataaagtgtt catgtatatc ggaaaatatg ctaagcagct tgaaatgctg agcatcgctt
     4861 ttgcaggtga cagcgataaa ggcatgatgc acgtaatgaa tgggtgtaag aacctgagga
     4921 aactggagat ccgtgacagc ccgtttgggg atgctgcgtt gttagggaat tttgctaggt
     4981 acgaaacgat gaggtccctg tggatgagca gttgtaatgt caccctaaag ggatgccaag
     5041 ttctagctag caaaatgccg atgcttaatg ttgaagtaat aaacgaaaga gatggttcaa
     5101 acgaaatgga agaaaatcac ggagatcttc cgaaggtaga gaaattatac gtgtaccgta
     5161 ccactgcggg cgcgagggac gacgcgccta attttgttaa aatactagga tcctaactcg
     5221 aggcgaattt cttatgattt atgattttta ttattaaata agttataaaa aaaataagtg
     5281 tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa attcttattc ttgagtaact
     5341 ctttcctgta ggtcaggttg ctttctcagg tatagcatga ggtcgctctt attgaccaca
     5401 cctctaccgg catgccgagc aaatgcctgc aaatcgctcc ccatttcgct gccaatgaga
     5461 cgacggggtc atcacggctc atcatgcgcc aaacaaatgt gtgcaataca cgctcggatg
     5521 actgcatgat gaccgcactg actggggaca gcagatccac ctaagcctgt gagagaagca
     5581 gacacccgac agatcaaggc agttaactag tgcactgcag taca
//
