LOCUS       sequence-469366-        2355 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; type3B; NS3 systems; DNCR2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pWN043
FEATURES             Location/Qualifiers
     source          1..2355
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(720..754)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(825..1484)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1399..1418
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1666..2254
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2155..2174
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat tcttcttccg atgaagagga agctagagag ctaatcgaaa gagcgaagga
       61 ggcagccgaa agagctcaag aggccgctga aaggaccggt gatccgagag tgagagaatt
      121 ggccagagaa ctgaagaggt tggcccagga agctgctgag gaagtcaaaa gggatcctag
      181 cagttccgat gtaaacgaag ctcttaagct gatagtggag gccattgaag cagcagtcga
      241 cgcgttagag gcggctgaga gaacggggga ccctgaagtg agggaattag cgcgtgaact
      301 agtcaggttg gctgtagaag ccgctgagga agtacaaaga aacccgtcca gttcagatgt
      361 taatgaagcg ctgcattcta ttgtttatgc gattgaggcg gcgattttcg ctcttgaagc
      421 ggctgagcgt accggtgatc ctgaggtaag agaactagca agagagttag tcagacttgc
      481 cgttgaagct gctgaagaag ttcaacgtaa tccatcttca aggaacgtag aacatgcctt
      541 gatgagaata gtgctagcca tatacctggc ggaagaaaac ctacgtgagg ccgaggagtc
      601 tggtgatcca gaaaagagag aaaaggcaag ggagagagtc agggaggctg tggaaagggc
      661 agaggaggtc cagcgtgatc caagtgggtg gctgaatcat ggatcctgag accagaccaa
      721 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
      781 ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc
      841 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
      901 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
      961 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
     1021 ctggtgaaac tcacccaggg attggctgaa acgaaaaaca tattctcaat aaacccttta
     1081 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
     1141 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
     1201 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
     1261 atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
     1321 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
     1381 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
     1441 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
     1501 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
     1561 aagttggaac ctcttacgtg cccgatcaat catgaccaaa atcccttaac gtgagttttc
     1621 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1681 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1741 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1801 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1861 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1921 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1981 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2041 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2101 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2161 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2221 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     2281 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     2341 tgtggataac cgtag
//
