LOCUS       sequence-469369-        5644 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; ConLS/R1 cassette; Auxin systems; 
            pRNR2-AmtR-NES-TIR1-U1-tADH1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pWN076
FEATURES             Location/Qualifiers
     source          1..5644
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(103..894,895..963))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     726..745
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(964..1068)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1171..1759
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             3452..3478
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     terminator      5243..5430
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5298..5322)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 gcggccgcga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
       61 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
      121 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
      181 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
      241 gcgggaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
      301 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
      361 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
      421 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
      481 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
      541 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
      601 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
      661 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
      721 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
      781 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
      841 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
      901 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
      961 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     1021 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     1081 aaaagtgcca cctgtcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1141 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1201 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1261 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1321 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1381 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1441 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1501 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1561 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1621 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     1681 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     1741 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     1801 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtgc
     1861 ggccgcccct gaattcgcat ctagatggta gagccacaaa cagccggtac aagcaacgat
     1921 ctccaggacc atctgaatca tgcgcggatg acacgaactc acgacggcga tcacagacat
     1981 taacccacag tacagacact gcgacaacgt ggcaattcgt cgcaataccg tctcactgaa
     2041 ctggccgata attgcagacg aacgagtcga acaagaagca ggcaaagttt agagcactgc
     2101 ccctccgcac tcaaaaaaga aaaaactagg aggaaaataa aattctcaac cacacaaaca
     2161 cataaacaca tacaaataca aatacaagct tatttacttg acatcgcgcg atcttccact
     2221 attcagcgcc gtccgccctc tctcgtgttt tttgtttacg cgacaactat gcgaaatccg
     2281 gagcaacggg caaccgtttg gggaaagacc acacccacgc gcgatcgcca tggcaacgag
     2341 gtcgcacacg ccccacaccc agacctccct gcgagcgggc atgggtacaa tgtccccgtt
     2401 gccacagaca ccacttcgta gcacagcgca gagcgtagcg tgttgttgct gctgacaaaa
     2461 gaaaattttt cttagcaaag caaaggaggg gaagcacggg cagatagcac cgtaccatac
     2521 ccttggaaac tcgaaatgaa cgaagcagga aatgagagaa tgagagtttt gtaggtatat
     2581 atagcggtag tgtttgcgcg ttaccatcat cttctggatc tatctattgt tcttttcctc
     2641 atcactttcc cctttttcgc tcttcttctt gtcttttatt tctttctttt ttttaattgt
     2701 tccctcgatt ggctatctac caaagaatcc aaacttaata cacgtattta tttgtccaat
     2761 taccagatct atggctggtg ctgtgggaag gccaaggagg tccgccccca ggagagcggg
     2821 gaaaaatcct cgtgaagaga tactggacgc atctgctgag ctttttacgc gtcaaggctt
     2881 tgctactact tcaactcacc agatagcaga tgcggtcggc attagacagg cttctctata
     2941 ttatcatttt ccgtcaaaaa cggagatttt ccttacacta cttaagtcta cagttgagcc
     3001 ttcaactgtc ttggcagagg atctatccac ccttgacgcc ggtcccgaga tgagattgtg
     3061 ggctatcgta gcgagtgagg tgcgtttatt gctatctaca aaatggaatg taggaaggct
     3121 ataccaattg cctatcgtgg gctcagagga gttcgctgaa taccattccc agagggaggc
     3181 gcttacaaat gtctttcgtg atcttgctac agaaattgta ggagatgacc ccagagcgga
     3241 gctgcccttc cacataacta tgagcgtgat agagatgcgt aggaacgatg gaaagattcc
     3301 aagtccatta agtgcagata gtcttccaga aaccgcaatc atgttggctg acgccagctt
     3361 ggccgtcctg ggagcacccc taccggccga tagggttgaa aagacgcttg aattgattaa
     3421 acaggcagac gcaaaggcaa gcggaagcgg actgcctccg ttagaaagac taacgctggg
     3481 ttcttctgga ggaggatcta cgcgtacata tttcccggaa aaggtagtaa agcatatatt
     3541 gtctttttta cccgctcaaa gggatcgtaa tacggtttca ctggtatgca aagtttggta
     3601 tgagattgag cgtctttcta ggaggggagt gtttgtgggc aattgttatg cggtccgtgc
     3661 cggccgtgtg gcagcaaggt ttccaaatgt aagggccctg acggtgaaag gaaaaccgca
     3721 ttttgctgac tttaacctgg tcccaccgga ttgggggggt tacgcgggtc cttggataga
     3781 agccgctgct cgtggatgcc acgggctaga agaactaagg atgaaaagga tggtagtatc
     3841 cgatgaatca ttggagctac tagctagaag cttcccgcgt ttcagggcgt tggtccttat
     3901 ttcttgtgag ggcttctcaa cagatggatt agctgccgtg gctagccatt gcaaacttct
     3961 gagagagctt gacttacaag agaacgaagt agaagaccgt ggccctcgtt ggctttcttg
     4021 ctttccagac agctgcacta gcctggtatc tttgaacttt gcgtgcatta agggtgaagt
     4081 caacgccggg agccttgaac gtttggtatc aagatctccg aatctgagaa gcctgcgttt
     4141 aaaccgttct gtctcagtag atacgctggc taaaatatta cttagaacac caaacttgga
     4201 ggatctggga accggcaact tgacagacga ctttcagaca gaaagctact tcaagctaac
     4261 ttctgcgttg gaaaaatgta agatgttgag gagcctgagt ggtttttggg acgccagtcc
     4321 tgtatgcctg tcattcattt atcctctgtg tgcgcagcta acgggactaa acctgtcata
     4381 cgctccgact ctagacgcct ctgatctgac taaaatgatc tctcgttgtg tgaagcttca
     4441 acgtctatgg gtacttgatt gtatcagtga caaagggtta caagtcgtag cctccagttg
     4501 taaagactta caggaactta gggtctttcc tagtgacttt tatgtagccg gatacagcgc
     4561 agttactgag gaaggtttag tggcggtgag cctaggatgc cccaaattga atagtctact
     4621 gtacttctgc catcaaatga ccaatgcagc cttagttacc gtcgctaaga attgtcctaa
     4681 cttcacccgt tttcgtttat gcatattgga gcctggcaaa ccagacgttg tcacttcaca
     4741 gccccttgac gaaggctttg gggccatagt tcgtgaatgt aaggggttgc aaaggctaag
     4801 tatttcaggg cttttaaccg ataaagtgtt catgtatatc ggaaaatatg ctaagcagct
     4861 tgaaatgctg agcatcgctt ttgcaggtga cagcgataaa ggcatgatgc acgtaatgaa
     4921 tgggtgtaag aacctgagga aactggagat ccgtgacagc ccgtttgggg atgctgcgtt
     4981 gttagggaat tttgctaggt acgaaacgat gaggtccctg tggatgagca gttgtaatgt
     5041 caccctaaag ggatgccaag ttctagctag caaaatgccg atgcttaatg ttgaagtaat
     5101 aaacgaaaga gatggttcaa acgaaatgga agaaaatcac ggagatcttc cgaaggtaga
     5161 gaaattatac gtgtaccgta ccactgcggg cgcgagggac gacgcgccta attttgttaa
     5221 aatactagga tcctaactcg aggcgaattt cttatgattt atgattttta ttattaaata
     5281 agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa
     5341 attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg tatagcatga
     5401 ggtcgctctt attgaccaca cctctaccgg catgccgagc aaatgcctgc aaatcgctcc
     5461 ccatttcgct gccaatgaga cgacggggtc atcacggctc atcatgcgcc aaacaaatgt
     5521 gtgcaataca cgctcggatg actgcatgat gaccgcactg actggggaca gcagatccac
     5581 ctaagcctgt gagagaagca gacacccgac agatcaaggc agttaactag tgcactgcag
     5641 taca
//
