LOCUS       sequence-469411-       15827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of EZH2, MBP-SUZ12, EED, RBBP4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15827)
  AUTHORS   Lai Z, Flanigan SF, Boudes M, Davidovich C
  TITLE     Modular cloning of multigene vectors for the baculovirus system and 
            yeast.
  JOURNAL   J Mol Biol. 2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943.
  PUBMED    39814169
REFERENCE   2  (bases 1 to 15827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943."
COMMENT     Sequence Label: pMB.BIG1a.PRC2
FEATURES             Location/Qualifiers
     source          1..15827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  892..1116
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1183..1716)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1905..1910,1911..1927,1928..1933))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        2358..2449
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    4880..5014
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        5113..5204
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             5256..6353
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="KTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEE
                     KFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLI
                     AYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAA
                     DGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGET
                     AMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLE
                     NYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFW
                     YAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      6210..6219
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     6327..6350
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             6387..6410
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     polyA_signal    8769..8903
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        9002..9093
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    10594..10728
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        10827..10918
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    12371..12505
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  12683..12848
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     CDS             13372..14163
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      14210..14665
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14297..14316)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14507..14528
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        14692..14796
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(14797..14865,14866..15657)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(15015..15034)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcagaccag ccgcgtaacc
      841 tggcaaaatc ggttacggtt gagtaataaa tggatgccct gcgtaagcgg gtgtgggcgg
      901 acaataaagt cttaaactga acaaaataga tctaaactat gacaataaag tcttaaacta
      961 gacagaatag ttgtaaactg aaatcagtcc agttatgctg tgaaaaagca tactggactt
     1021 ttgttatggc taaagcaaac tcttcatttt ctgaagtgca aattgcccgt cgtattaaag
     1081 aggggcgtgg ccaagggcat ggtaaagact atattcgcgg cgttgtgaca atttaccgaa
     1141 caactccgcg gccgggaagc cgatctcggc ttgaacgaat tgttaggtgg cggtacttgg
     1201 gtcgatatca aagtgcatca cttcttcccg tatgcccaac tttgtataga gagccactgc
     1261 gggatcgtca ccgtaatctg cttgcacgta gatcacataa gcaccaagcg cgttggcctc
     1321 atgcttgagg agattgatga gcgcggtggc aatgccctgc ctccggtgct cgccggagac
     1381 tgcgagatca tagatataga tctcactacg cggctgctca aacttgggca gaacgtaagc
     1441 cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca agcgcgatga atgtcttact
     1501 acggagcaag ttcccgaggt aatcggagtc cggctgatgt tgggagtagg tggctacgtc
     1561 gccgaactca cgaccgaaaa gatcaagagc agcccgcatg gatttgactt ggtcagggcc
     1621 gagcctacat gtgcgaatga tgcccatact tgagccacct aactttgttt tagggcgact
     1681 gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt gctgctccat aacatcaaac
     1741 atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtacaaaaa
     1801 aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca
     1861 aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagttta cgaaccgaac
     1921 aggcttatgt caactgggtt cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa
     1981 ccttgggcag cagcgaagtc gaggcatttc tgtcctggct ggcgaacgag cgcaaggttt
     2041 cggactccac gcatcgtcag gcattggcgg ccttgctgtt cttctacggc aaggtgctgt
     2101 gcacggatct gccctggctt caggagatcg gaagacctcg gccgtcgcgg cgcttgccgg
     2161 tggtgctgac cccggatgaa gtggttcgca tcctcggttt tctggaaggc gagcatcgtt
     2221 tgttcgccca ggactctagc tatagttccc tgaattcgca tctagagttt aaacgctcta
     2281 tggtctaaag tttctgaact ggccgataat tgcagacgaa cgctagtggt tggctacata
     2341 ctccggaata ttaatagatc atggagataa ttaaaatgat aaccatctcg caaataaata
     2401 agtattttac tgttttcgta acagttttgt aataaaaaaa cctataaata ttccggatta
     2461 ttcataccgt cccaccatcg ggcgctccgg tatgggccag actgggaaga aatctgagaa
     2521 gggaccagtt tgttggcgga agcgtgtaaa atcagagtac atgcgactga gacagctcaa
     2581 gaggttcaga cgagctgatg aagtaaagag tatgtttagt tccaatcgtc agaaaatttt
     2641 ggaaagaacg gaaatcttaa accaagaatg gaaacagcga aggatacagc ctgtgcacat
     2701 cctgacttct gtgagctcat tgcgcgggac tagggagtgt tcggtgacca gtgacttgga
     2761 ttttccaaca caagtcatcc cattaaagac tctgaatgca gttgcttcag tacccataat
     2821 gtattcttgg tcacccctac agcagaattt tatggtggaa gatgaaactg ttttacataa
     2881 cattccttat atgggagatg aagttttaga tcaggatggt actttcattg aagaactaat
     2941 aaaaaattat gatgggaaag tacacgggga tagagaatgt gggtttataa atgatgaaat
     3001 ttttgtggag ttggtgaatg cccttggtca atataatgat gatgacgatg atgatgatgg
     3061 agatgatcct gaagaaagag aagaaaagca gaaagacctg gaggatcacc gagatgataa
     3121 agaaagccgc ccacctcgga aatttccttc tgataaaatt tttgaagcca tttcctcaat
     3181 gtttccagat aagggcacag cagaagaact aaaggaaaaa tataaagaac tcaccgaaca
     3241 gcagctccca ggcgcacttc ctcctgaatg tacccccaac atagatggac caaatgctaa
     3301 atctgttcag agagagcaaa gcttacactc ctttcatacg cttttctgta ggcgatgttt
     3361 taaatatgac tgcttcctac atcgtaagtg caattattct tttcatgcaa cacccaacac
     3421 ttataagcgg aagaacacag aaacagctct agacaacaaa ccttgtggac cacagtgtta
     3481 ccagcatttg gagggagcaa aggagtttgc tgctgctctc accgctgagc ggataaagac
     3541 cccaccaaaa cgtccaggag gccgcagaag aggacggctt cccaataaca gtagcaggcc
     3601 cagcaccccc accattaatg tgctggaatc aaaggataca gacagtgata gggaagcagg
     3661 gactgaaacg gggggagaga acaatgataa agaagaagaa gagaagaaag atgaaacttc
     3721 gagctcctct gaagcaaatt ctcggtgtca aacaccaata aagatgaagc caaatattga
     3781 acctcctgag aatgtggagt ggagtggtgc tgaagcctca atgtttagag tcctcattgg
     3841 cacttactat gacaatttct gtgccattgc taggttaatt gggaccaaaa catgtagaca
     3901 ggtgtatgag tttagagtca aagaatctag catcatagct ccagctcccg ctgaggatgt
     3961 ggatactcct ccaaggaaaa agaagaggaa acaccggttg tgggctgcac actgcagaaa
     4021 gatacagctg aaaaaggacg gctcctctaa ccatgtttac aactatcaac cctgtgatca
     4081 tccacggcag ccttgtgaca gttcgtgccc ttgtgtgata gcacaaaatt tttgtgaaaa
     4141 gttttgtcaa tgtagttcag agtgtcaaaa ccgctttccg ggatgccgct gcaaagcaca
     4201 gtgcaacacc aagcagtgcc cgtgctacct ggctgtccga gagtgtgacc ctgacctctg
     4261 tcttacttgt ggagccgctg accattggga cagtaaaaat gtgtcctgca agaactgcag
     4321 tattcagcgg ggctccaaaa agcatctatt gctggcacca tctgacgtgg caggctgggg
     4381 gatttttatc aaagatcctg tgcagaaaaa tgaatttatc tcagaatact gtggagagat
     4441 tatttctcaa gatgaagctg acagaagagg gaaagtgtat gataaataca tgtgcagctt
     4501 tctgttcaac ttgaacaatg attttgtggt ggatgcaacc cgcaagggta acaaaattcg
     4561 ttttgcaaat cattcggtaa atccaaactg ctatgcaaaa gttatgatgg ttaacggtga
     4621 tcacaggata ggtatttttg ccaagagagc catccagact ggcgaagagc tgttttttga
     4681 ttacagatac agccaggctg atgccctgaa gtatgtcggc atcgaaagag aaatggaaat
     4741 ccctggatcc taatgagtcg agaagtacta gaggatcata atcagccata ccacatttgt
     4801 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     4861 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     4921 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     4981 caaactcatc aatgtatctt atcatgtctg gatctgatca ctgatatcgc ctaggagatc
     5041 cggggctgga tgcacacact ggcttaagac gacaacgcta gtggttggct acatactccg
     5101 gaatattaat agatcatgga gataattaaa atgataacca tctcgcaaat aaataagtat
     5161 tttactgttt tcgtaacagt tttgtaataa aaaaacctat aaatattccg gattattcat
     5221 accgtcccac catcgggcgc ggaagaatgg gtaccaaaac tgaagaaggt aaactggtaa
     5281 tctggattaa cggcgataaa ggctataacg gtttggctga agtcggtaag aaattcgaga
     5341 aagataccgg aattaaagtc accgttgagc atccggataa actggaagag aaattcccac
     5401 aggttgcggc aactggcgat ggccctgaca ttatcttctg ggcacacgac cgctttggtg
     5461 gctacgctca atctggcctg ttggctgaaa tcaccccgga caaagcgttc caggacaagc
     5521 tgtatccgtt tacctgggat gccgtacgtt acaacggcaa gctgattgct tacccgatcg
     5581 ctgttgaagc gttatcgctg atttataaca aagatttgct gccgaacccg ccaaaaacct
     5641 gggaagagat cccggcgctg gataaagaac tgaaagcgaa aggtaagagc gcgctgatgt
     5701 tcaacctgca agaaccgtac ttcacctggc cgctgattgc tgctgacggg ggttatgcgt
     5761 tcaagtatga aaacggcaag tacgacatta aagacgtggg cgtggataac gctggcgcga
     5821 aagcgggtct gaccttcctg gttgacctga ttaaaaacaa acacatgaat gcagacaccg
     5881 attactccat cgcagaagct gcctttaata aaggcgaaac agcgatgacc atcaacggcc
     5941 cgtgggcatg gtccaacatc gacaccagca aagtgaatta tggtgtaacg gtactgccga
     6001 ccttcaaggg tcaaccatcc aaaccgttcg ttggcgtgct gagcgcaggt attaacgccg
     6061 ccagtccgaa caaagagctg gcaaaagagt tcctcgaaaa ctatctgctg actgatgaag
     6121 gtctggaagc ggttaataaa gacaaaccgc tgggtgccgt agcgctgaag tcttacgagg
     6181 aagagttggc gaaagatcca cgtattgccg ccaccatgga aaacgcccag aaaggtgaaa
     6241 tcatgccgaa catcccgcag atgtccgctt tctggtatgc cgtgcgtact gcggtgatca
     6301 acgccgccag cggtcgtcag actgtcgatg aagccctgaa agacgcgcag actgccgcag
     6361 gcaccgatta cgatatccca acgaccctgg aagttctgtt ccaggggccc tccggtatgg
     6421 cgcctcagaa gcacggcggt gggggagggg gcggctcggg gcccagcgcg gggtccgggg
     6481 gaggcggctt cgggggttcg gcggcggtgg cggcggcgac ggcttcgggc ggcaaatccg
     6541 gcggcgggag ctgtggaggg ggtggcagtt actcggcctc ctcctcctcc tccgcggcgg
     6601 cagcggcggg ggctgcggtg ttaccggtga agaagccgaa aatggagcac gtccaggctg
     6661 accacgagct tttcctccag gcctttgaga agccaacaca aatctataga tttcttcgaa
     6721 ctcggaatct catagcacca atatttttgc acagaactct tacttacatg tctcatcgaa
     6781 actccagaac aaacatcaaa aggaaaacat ttaaagttga tgatatgtta tcaaaagtag
     6841 agaaaatgaa aggagagcaa gaatctcata gcttgtcagc tcatttgcag cttacgttta
     6901 ctggtttctt ccacaaaaat gataagccat caccaaactc agaaaatgaa caaaattctg
     6961 ttaccctgga agtcctgctt gtgaaagttt gccacaaaaa aagaaaggat gtaagttgtc
     7021 caataaggca agttcccaca ggtaaaaagc aggtgccttt gaatcctgac ctcaatcaaa
     7081 caaaacccgg aaatttcccg tcccttgcag tttccagtaa tgaatttgaa cctagtaaca
     7141 gccatatggt gaagtcttac tcgttgctat ttagagtgac tcgtccagga agaagagagt
     7201 ttaatggaat gattaatgga gaaaccaatg aaaatattga tgtcaatgaa gagcttccag
     7261 ccagaagaaa acgaaatcgt gaggatgggg aaaagacatt tgttgcacaa atgacagtat
     7321 ttgataaaaa caggcgctta cagcttttag atggggaata tgaagtagcc atgcaggaaa
     7381 tggaagaatg tccaataagc aagaaaagag caacatggga gactattctt gatgggaaga
     7441 ggctgcctcc attcgaaaca ttttctcagg gacctacgtt gcagttcact cttcgttgga
     7501 caggagaaac caatgataaa tctacggctc ctattgccaa acctcttgcc actagaaatt
     7561 cagagagtct ccatcaggaa aacaagcctg gttcagttaa acctactcaa actattgctg
     7621 ttaaagaatc attgactaca gacctacaaa caagaaaaga aaaggatact ccaaatgaaa
     7681 accgacaaaa attaagaata ttttatcagt ttctctataa caacaataca aggcaacaaa
     7741 ctgaagcaag agatgacctg cattgccctt ggtgtactct gaactgccgc aaactttata
     7801 gtttactcaa gcatcttaaa ctctgccata gcagatttat cttcaactat gtttatcatc
     7861 caaaaggtgc taggatagat gtttctatca atgagtgtta tgatggctcc tatgcaggaa
     7921 atcctcagga tattcatcgc caacctggat ttgcttttag tcgcaacgga ccagttaaga
     7981 gaacacctat cacacatatt cttgtgtgca ggccaaaacg aacaaaagca agcatgtctg
     8041 aatttcttga atctgaagat ggggaagtag aacagcaaag aacatatagt agtggccaca
     8101 atcgtctgta tttccatagt gatacctgct tacctctccg tccacaagaa atggaagtag
     8161 atagtgaaga tgaaaaagat cctgaatggc taagagaaaa aaccattaca caaattgaag
     8221 agttttctga tgttaatgaa ggagagaaag aagtgatgaa actctggaat ctccatgtca
     8281 tgaagcatgg gtttattgct gacaatcaaa tgaatcatgc ctgtatgctg tttgtagaaa
     8341 attatggaca gaaaataatt aagaagaatt tatgtcgaaa cttcatgctt catctagtca
     8401 gcatgcatga ctttaatctt attagcataa tgtcaataga taaagctgtt accaagctcc
     8461 gtgaaatgca gcaaaaatta gaaaaggggg aatctgcttc ccctgcaaac gaagaaataa
     8521 ctgaagaaca aaatgggaca gcaaatggat ttagtgaaat taactcaaaa gagaaagctt
     8581 tggaaacaga tagtgtctca ggggtttcaa aacagagcaa aaaacaaaaa ctcggatcct
     8641 aatgagtcga gaagtactag aggatcataa tcagccatac cacatttgta gaggttttac
     8701 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     8761 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     8821 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     8881 atgtatctta tcatgtctgg atctgatcac tgatatcgcc taggagatcc ggggctggat
     8941 gcacacactg gcttaagacg acaacgctag tggttggcta catactccgg aatattaata
     9001 gatcatggag ataattaaaa tgataaccat ctcgcaaata aataagtatt ttactgtttt
     9061 cgtaacagtt ttgtaataaa aaaacctata aatattccgg attattcata ccgtcccacc
     9121 atcgggcgct ccggtatgtc cgagagggaa gtgtcgactg cgccggcggg aacagacatg
     9181 cctgcggcca agaagcagaa gctgagcagt gacgagaaca gcaatccaga cctctctgga
     9241 gatgagaatg atgacgctgt cagtatagaa agtggtacaa acactgaacg ccctgataca
     9301 cctacaaaca cgccaaatgc acctggaagg aaaagttggg gaaagggaaa atggaagtca
     9361 aagaaatgca aatattcttt caaatgtgta aatagtctca aggaagatca taaccaacca
     9421 ttgtttggag ttcagtttaa ctggcacagt aaagaaggag atccattagt gtttgcaact
     9481 gtaggaagca acagagttac cttgtatgaa tgtcattcac aaggagaaat ccggttgttg
     9541 caatcttacg tggatgctga tgctgatgaa aacttttaca cttgtgcatg gacctatgat
     9601 agcaatacga gccatcctct gctggctgta gctggatcta gaggcataat taggataata
     9661 aatcctataa caatgcagtg tataaagcac tatgttggcc atggaaatgc tatcaatgag
     9721 ctgaaattcc atccaagaga tccaaatctt ctcctgtcag taagtaaaga tcatgcttta
     9781 cgattatgga atatccagac ggacactctg gtggcaatat ttggaggcgt agaagggcac
     9841 agagatgaag ttctaagtgc tgattatgat cttttgggtg aaaaaataat gtcctgtggt
     9901 atggatcatt ctcttaaact ttggaggatc aattcaaaga gaatgatgaa tgcaattaag
     9961 gaatcttatg attataatcc aaataaaact aacaggccat ttatttctca gaaaatccat
    10021 tttcctgatt tttctaccag agacatacat aggaattatg ttgattgtgt gcgatggtta
    10081 ggcgatttga tactttctaa gtcttgtgaa aatgccattg tgtgctggaa acctggcaag
    10141 atggaagatg atatagataa aattaaaccc agtgaatcta atgtgactat tcttgggcga
    10201 tttgattaca gccagtgtga catttggtac atgaggtttt ctatggattt ctggcaaaag
    10261 atgcttgcat tgggcaatca agttggcaaa ctttatgttt gggatttaga agtagaagat
    10321 cctcataaag ccaaatgtac aacactgact catcataaat gtggtgctgc tattcgacaa
    10381 accagtttta gcagggatag cagcattctt atagctgttt gtgatgatgc cagtatttgg
    10441 cgctgggatc gacttcgagg atcctaatga gtcgagaagt actagaggat cataatcagc
    10501 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
    10561 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
    10621 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    10681 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctg atcactgata
    10741 tcgcctagga gatccggggc tggatgcaca cactggctta agacgacaac gctagtggtt
    10801 ggctacatac tccggaatat taatagatca tggagataat taaaatgata accatctcgc
    10861 aaataaataa gtattttact gttttcgtaa cagttttgta ataaaaaaac ctataaatat
    10921 tccggattat tcataccgtc ccaccatcgg gcgctccggt atggccgaca aggaagcagc
    10981 cttcgacgac gcagtggaag aacgagtgat caacgaggaa tacaaaatat ggaaaaagaa
    11041 cacccctttt ctttatgatt tggtgatgac ccatgctctg gagtggccca gcctaactgc
    11101 ccagtggctt ccagatgtaa ccagaccaga agggaaagat ttcagcattc atcgacttgt
    11161 cctggggaca cacacatcgg atgaacaaaa ccatcttgtt atagccagtg tgcagctccc
    11221 taatgatgat gctcagtttg atgcgtcaca ctacgacagt gagaaaggag aatttggagg
    11281 ttttggttca gttagtggaa aaattgaaat agaaatcaag atcaaccatg aaggagaagt
    11341 aaacagggcc cgttatatgc cccagaaccc ttgtatcatc gcaacaaaga ctccttccag
    11401 tgatgttctt gtttttgact atacaaaaca tccttctaaa ccagatcctt ctggagagtg
    11461 caacccagac ttgcgactcc gtggacatca gaaggaaggc tatgggcttt cttggaaccc
    11521 aaatctcagt gggcacttac ttagtgcttc agatgaccat accatctgcc tgtgggacat
    11581 cagtgccgtt ccaaaggagg gaaaagtggt agatgcgaag accatcttta cagggcatac
    11641 ggcagtagta gaagatgttt cctggcatct actccatgag tctctgtttg ggtcagttgc
    11701 tgatgatcag aaacttatga tttgggatac tcgttcaaac aatacttcca aaccaagcca
    11761 ctcagttgat gctcacactg ctgaagtgaa ctgcctttct ttcaatcctt atagtgagtt
    11821 cattcttgcc acaggatcag ctgacaagac tgttgccttg tgggatctga gaaatctgaa
    11881 acttaagttg cattcctttg agtcacataa ggatgaaata ttccaggttc agtggtcacc
    11941 tcacaatgag actattttag cttccagtgg tactgatcgc agactgaatg tctgggattt
    12001 aagtaaaatt ggagaggaac aatccccaga agatgcagaa gacgggccac cagagttgtt
    12061 gtttattcat ggtggtcata ctgccaagat atctgatttc tcctggaatc ccaatgaacc
    12121 ttgggtgatt tgttctgtat cagaagacaa tatcatgcaa gtgtggcaaa tggcagagaa
    12181 catttataat gatgaagacc ctgaaggaag cgttgatcca gaaggacaag ggtccggatc
    12241 ctaatgagtc gagaagtact agaggatcat aatcagccat accacatttg tagaggtttt
    12301 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
    12361 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    12421 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    12481 caatgtatct tatcatgtct ggatctgatc actgatatcg cctaggagat ccggggctgg
    12541 gtagactacc catgagtcac aatgaacgac tagagatcta tgtgaggatc ctaactcgag
    12601 atcgagctga gcaaaactat atctcaatcg gggtttaaac gttacagaga tgttacgaac
    12661 cactagtgca ctgcagtaca tcaaccagat aagtgaaatc tagttccaaa ctattttgtc
    12721 atttttaatt ttcgtattag cttacgacgc tacacccagt tcccatctat tttgtcactc
    12781 ttccctaaat aatccttaaa aactccattt ccacccctcc cagttcccaa ctattttgtc
    12841 cgcccacagc ggggcatttt tcttcctgtt atgtttttaa tcaaacatcc tgccaactcc
    12901 atgtgacaaa ccgtcatctt cggctacttt ttctctgtca cagaatgaaa atttttctgt
    12961 catctcttcg ttattaatgt ttgtaattga ctgaatatca acgcttattt gcagcctgaa
    13021 ctgggaggca gaataaatga tcatatcgtc aattattacc tccacgggga gagcctgagc
    13081 aaactggcct caggcatttg agaagcacac ggtcacactg cttccggtag tcaataaacc
    13141 ggtaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
    13201 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg
    13261 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
    13321 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgagggaa
    13381 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat
    13441 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag
    13501 ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg
    13561 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc
    13621 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct
    13681 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag
    13741 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt
    13801 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt
    13861 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag
    13921 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg
    13981 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc
    14041 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca
    14101 gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa
    14161 taatgtctaa caattcgttc aagccgacgg attggcgaat ggggggagta cgcgccctgt
    14221 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
    14281 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
    14341 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
    14401 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
    14461 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
    14521 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
    14581 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
    14641 aacaaaatat taacgtttac aatttcaggt ggcacttttc ggggaaatgt gcgcggaacc
    14701 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
    14761 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
    14821 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
    14881 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
    14941 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
    15001 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
    15061 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
    15121 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
    15181 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
    15241 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
    15301 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
    15361 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
    15421 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
    15481 attgctgata aatctggagc cggtgagcgt ggttctcgcg gtatcattgc agcactgggg
    15541 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
    15601 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
    15661 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
    15721 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
    15781 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttc
//
