LOCUS       sequence-469429-       23295 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA vector for expression of the two protein components of the 
            MoonTag system (dCas9-24XGP41 and NbGP41P-GFP-AvrXa10-GB1) with 
            optimized activation domain (AvrXa10), targeting Arabidopsis FT 
            locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 23295)
  AUTHORS   Zinselmeier MH, Casas-Mollano JA, Cors J, Sychla A, Heinsch SC, 
            Voytas DF, Smanski MJ
  TITLE     Optimized dCas9 programmable transcriptional activators for plants.
  JOURNAL   Plant Biotechnol J. 2024 Nov;22(11):3202-3204. doi: 
            10.1111/pbi.14441. Epub 2024 Jul 26.
  PUBMED    39058765
REFERENCE   2  (bases 1 to 23295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14441";
            journalName: "Plant Biotechnol J"; date: "2024-11"; volume: "22"; 
            issue: "11"; pages: "3202-3204"
COMMENT     Sequence Label: MT-AvrXa10-FT
FEATURES             Location/Qualifiers
     source          1..23295
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(5..22)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5..21)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14..36)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    224..248
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1549..2178
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2607..3680
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3746..3940
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4176..4198)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4284..4424
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4339..4358
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4439..4456)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4610..5198)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4690..4709)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5285..6079)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    6504..6528
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    6606..6780
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(6837..7634)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6948..6967)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7558..7577
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(7697..8373)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(7740..7760)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    8564..8585
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8600..8605,8606..8623,8624..8630)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8638..8654
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8643..8665
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8662..8678
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8662..8678
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             join(10059..10085,10086..10088,10089..12575,12576..12578,
                     12579..14162)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             10059..14162
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             14166..14192
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             14172..14198
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             14211..14231
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             14238..14258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             14268..14288
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             14355..14399
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             14415..14459
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             14475..14519
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             14535..14579
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             14595..14639
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             14655..14699
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             14715..14759
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             14775..14819
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             14835..14879
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             14895..14939
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             14955..14999
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             15015..15059
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             15075..15119
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             15135..15179
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             15195..15239
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             15255..15299
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             15315..15359
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             15375..15419
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             15435..15479
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             15495..15539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             15555..15599
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             15615..15659
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             15675..15719
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             15735..15779
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     terminator      15820..16068
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_RNA        16563..16638
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        17652..17727
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             18955..19650
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     CDS             21454..21474
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             21871..22581
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             21871..22581
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(21898..21926)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     22521..22541
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             22807..22968
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
ORIGIN
        1 tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
       61 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
      121 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ctagagcagc ttgagcttgg
      181 atcagattgt cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc
      241 gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa
      301 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc
      361 aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
      421 agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc
      481 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg
      541 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg
      601 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg
      661 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg
      721 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc
      781 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg
      841 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt
      901 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga
      961 gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc
     1021 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg
     1081 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg
     1141 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg
     1201 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca
     1261 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc
     1321 cgggtacgtg ttcgagccgc ccgcgcacgg ctcaaccgtg cggctgcatg aaatcctggc
     1381 cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga
     1441 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc
     1501 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc
     1561 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc
     1621 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc
     1681 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg
     1741 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg
     1801 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg
     1861 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag
     1921 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa
     1981 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt
     2041 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt
     2101 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt
     2161 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc
     2221 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag
     2281 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga
     2341 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc
     2401 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga
     2461 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga
     2521 ggaacgggcg gttggccagg cgtaagcggc tgggttgcct gccggccctg caatggcact
     2581 ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa
     2641 tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc
     2701 ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa
     2761 tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca
     2821 agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata
     2881 gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg
     2941 aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca
     3001 tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca
     3061 tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg
     3121 cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag
     3181 aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga
     3241 acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa
     3301 agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg
     3361 agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg
     3421 atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag
     3481 ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt
     3541 tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg
     3601 aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg
     3661 aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg
     3721 aaaaaggtcg aaaaggcctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt
     3781 acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac
     3841 acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa
     3901 aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag
     3961 cccaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt
     4021 cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc
     4081 taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca
     4141 ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggaa
     4201 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     4261 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
     4321 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     4381 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggccctct tccgcttcct
     4441 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     4501 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     4561 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     4621 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     4681 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     4741 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     4801 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     4861 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     4921 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     4981 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     5041 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     5101 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     5161 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     5221 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcattcta
     5281 ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga
     5341 tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg
     5401 accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa
     5461 taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg
     5521 aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat
     5581 ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca
     5641 gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata
     5701 tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc
     5761 tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat
     5821 tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca
     5881 gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt
     5941 ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag
     6001 acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg
     6061 atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc
     6121 ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct
     6181 taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc
     6241 gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc atcgttacaa
     6301 tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt
     6361 cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg
     6421 acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg
     6481 tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt
     6541 agacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc cgaattaatt
     6601 cgggggatct ggattttagt actggatttt ggttttagga attagaaatt ttattgatag
     6661 aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga
     6721 aaccctatag gaaccctaat tcccttatct gggaactact cacacattat tatggagaaa
     6781 ctcgagcttg tcgatcgact ctagctagag gatcgatccg aaccccagag tcccgctcag
     6841 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
     6901 taaagcacga ggaagcggtc agcccattcg ccgccaagtt cttcagcaat atcacgggta
     6961 gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca
     7021 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg tgtcacgacg
     7081 agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc
     7141 ccctgatgtt cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
     7201 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
     7261 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
     7321 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
     7381 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
     7441 gcctcgtcct ggagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
     7501 cgcccctgcg ctgacagccg aaacacggcg gcatcagagc agccgattgt ctgttgtgcc
     7561 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
     7621 tgttcaatcc ccatggtcga tcgacagatc tgcgaaagct cgagagagat agatttgtag
     7681 agagagactg gtgatttcag cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg
     7741 aagggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac
     7801 atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg
     7861 tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc
     7921 ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga
     7981 agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa
     8041 aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga
     8101 gagtgtcgtg ctccaccatg ttcacatcaa tccacttgct ttgaagacgt ggttggaacg
     8161 tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca
     8221 gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct
     8281 tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg
     8341 tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta
     8401 tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttggc aagctgctct
     8461 agccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     8521 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     8581 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     8641 tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat tcgagctcgg
     8701 tacccgggga tcggcgcgcc ggaggtcgag ctgcaggtca acggatcagg atattcttgt
     8761 ttaagatgtt gaactctatg gaggtttgta tgaactgatg atctaggacc ggataagttc
     8821 ccttcttcat agcgaactta ttcaaagaat gttttgtgta tcattcttgt tacattgtta
     8881 ttaatgaaaa aatattattg gtcattggac tgaacacgag tgttaaatat ggaccaggcc
     8941 ccaaataaga tccattgata tatgaattaa ataacaagaa taaatcgagt caccaaacca
     9001 cttgcctttt ttaacgagac ttgttcacca acttgataca aaagtcatta tcctatgcaa
     9061 atcaataatc atacaaaaat atccaataac actaaaaaat taaaagaaat ggataatttc
     9121 acaatatgtt atacgataaa gaagttactt ttccaagaaa ttcactgatt ttataagccc
     9181 acttgcatta gataaatggc aaaaaaaaac aaaaaggaaa agaaataaag cacgaagaat
     9241 tctagaaaat acgaaatacg cttcaatgca gtgggaccca cggttcaatt attgccaatt
     9301 ttcagctcca ccgtatattt aaaaaataaa acgataatgc taaaaaaata taaatcgtaa
     9361 cgatcgttaa atctcaacgg ctggatctta tgacgaccgt tagaaattgt ggttgtcgac
     9421 gagtcagtaa taaacggcgt caaagtggtt gcagccggca cacacgagtc gtgtttatca
     9481 actcaaagca caaatacttt tcctcaacct aaaaataagg caattagcca aaaacaactt
     9541 tgcgtgtaaa caacgctcaa tacacgtgtc attttattat tagctattgc ttcaccgcct
     9601 tagctttctc gtgacctagt cgtcctcgtc ttttcttctt cttcttctat aaaacaatac
     9661 ccaaagagct cttcttcttc acaattcaga tttcaatttc tcaaaatctt aaaaactttc
     9721 tctcaattct ctctaccgtg atcaaggtaa atttctgtgt tccttattct ctcaaaatct
     9781 tcgattttgt tttcgttcga tcccaatttc gtatatgttc tttggtttag attctgttaa
     9841 tcttagatcg aacacgattt tctgggtttg atcgttagat atcatcttaa ttctcgatta
     9901 gggtttcata gatatcatcc gatttgttca aataatttga gttttgtcga ataattactc
     9961 ttcgatttgt gatttctatc tagatctggt gttagtttct agtttgtgcg atcgaatttg
    10021 tcgattaatc tgagtttttc tgattaacag gaatgaacat ggacaagaag tacagcatcg
    10081 gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaggtgc
    10141 ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga
    10201 tcggcgccct gctgttcgac agcggagaaa cagccgaggc cacccggctg aagagaaccg
    10261 ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag atcttcagca
    10321 acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc ttcctggtgg
    10381 aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac gaggtggcct
    10441 accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac agcaccgaca
    10501 aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc cggggccact
    10561 tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg ttcatccagc
    10621 tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc ggcgtggacg
    10681 ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat ctgatcgccc
    10741 agctgcccgg cgagaagaag aatggcctgt tcggcaacct gattgccctg agcctgggcc
    10801 tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg cagctgagca
    10861 aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac cagtacgccg
    10921 acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac atcctgagag
    10981 tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga tacgacgagc
    11041 accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca
    11101 aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacatcgat ggcggagcca
    11161 gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac ggcaccgagg
    11221 aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc ttcgacaacg
    11281 gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg cggcaggaag
    11341 atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg accttccgca
    11401 tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg atgaccagaa
    11461 agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag ggcgccagcg
    11521 cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac gagaaggtgc
    11581 tgcccaagca cagcctgctg tacgagtact tcaccgtgta caacgagctg accaaagtga
    11641 aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag aaaaaagcca
    11701 tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg aaagaggact
    11761 acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa gatcggttca
    11821 acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag gacttcctgg
    11881 acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca ctgtttgagg
    11941 acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac gacaaagtga
    12001 tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg aagctgatca
    12061 acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag tccgacggct
    12121 tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt aaagaggaca
    12181 tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt gccaatctgg
    12241 ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg gacgagctcg
    12301 tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc agagagaacc
    12361 agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc gaagagggca
    12421 tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc cagctgcaga
    12481 acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg gaccaggaac
    12541 tggacatcaa ccggctgtcc gactacgatg tggacgctat cgtgcctcag agctttctga
    12601 aggacgactc catcgataac aaagtgctga ctcggagcga caagaaccgg ggcaagagcg
    12661 acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgc cagctgctga
    12721 atgccaagct gattacccag aggaagttcg acaatctgac caaggccgag agaggcggcc
    12781 tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc cggcagatca
    12841 caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac gagaacgaca
    12901 aactgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga
    12961 aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct
    13021 acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg gaaagcgagt
    13081 tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag agcgagcagg
    13141 aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac tttttcaaga
    13201 ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg
    13261 aaacaggcga gatcgtgtgg gataagggcc gggactttgc caccgtgcgg aaagtgctgt
    13321 ctatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag
    13381 agtctatcct gcccaagagg aacagcgaca agctgatcgc cagaaagaag gactgggacc
    13441 ctaagaagta cggcggcttc gacagcccca ccgtggccta ttctgtgctg gtggtggcca
    13501 aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg gggatcacca
    13561 tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc aagggctaca
    13621 aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc gagctggaaa
    13681 acggccggaa gagaatgctg gcctctgccg gcgaactgca gaagggaaac gaactggccc
    13741 tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag ctgaagggct
    13801 cccccgagga taatgagcag aaacagctgt ttgtggaaca gcacaaacac tacctggacg
    13861 agatcatcga gcagatcagc gagttctcca agagagtgat cctggccgac gctaatctgg
    13921 acaaggtgct gagcgcctac aacaagcaca gagacaagcc tatcagagag caggccgaga
    13981 atatcatcca cctgtttacc ctgaccaatc tgggagcccc tgccgccttc aagtactttg
    14041 acaccaccat cgaccggaag aggtacacca gcaccaaaga ggtgctggac gccaccctga
    14101 tccaccagag catcaccggc ctgtacgaga cacggatcga cctgtctcag ctgggaggcg
    14161 acgcctatcc ctatgacgtg cccgattatg ccagcctggg cagcggctcc cccaagaaaa
    14221 aacgcaaggt ggaagatcct aagaaaaagc ggaaagtgga agatgctcca aagaagaaga
    14281 gaaaggtcga cggcattggt agtgggagca acggcagcag cggatccctt aagcttgcca
    14341 ccatgcgtac gggcaagaat gagcaggaac tgctggaact ggacaaatgg gcaagcctgg
    14401 ggagcggctc cggaaagaac gagcaggaac tgctggaact ggacaagtgg gcttctctgg
    14461 gctccgggtc cgggaaaaac gaacaggagc tgctggagct ggataagtgg gccagcctgg
    14521 gctccgggtc cgggaagaat gaacaggaac tgctggaact ggataaatgg gcatctctgg
    14581 gcagcgggtc cggaaagaat gaacaggagc tgctggaact ggacaagtgg gcatccctgg
    14641 ggagcgggtc cggaaagaat gaacaggaac tgctggaact ggataagtgg gcatccctgg
    14701 gaagcggctc cggaaaaaac gagcaggagc tgctggagct ggacaagtgg gcctctctgg
    14761 gctccggctc cgggaagaat gaacaggagc tgctggaact ggataagtgg gcatctctgg
    14821 ggtccggaag cggtaaaaac gagcaggaac tgctggaact ggacaagtgg gcaagcctgg
    14881 ggtctggctc cggcaaaaac gaacaggaac tgctggaact ggacaaatgg gccagcctgg
    14941 gctccgggtc cgggaaaaat gagcaggaac tgctggaact ggataaatgg gcgagcctgg
    15001 gcagcgggtc cggaaaaaac gaacaggagc tgctggagct ggacaaatgg gcgagtctgg
    15061 gctccggaag cggcaagaat gaacaggaac tgctggagct ggacaaatgg gccagcctgg
    15121 gcagcggctc cggaaagaat gagcaggaac tgctggagct ggacaaatgg gcatctctgg
    15181 gctccggctc cgggaaaaac gagcaggaac tgctggagct ggacaagtgg gcttccctgg
    15241 gcagcggctc cggaaaaaat gaacaggaac tgctggaact ggacaagtgg gccagcctgg
    15301 ggtccgggtc tgggaagaac gagcaggaac tgctggagct ggacaagtgg gcatctctgg
    15361 ggtctggctc tggcaagaac gagcaggaac tgctggaact ggacaagtgg gcgagcctgg
    15421 gcagcgggtc tggcaagaat gaacaggagc tgctggaact ggacaaatgg gccagcctgg
    15481 ggtctgggtc tggaaagaat gagcaggaac tgctggagct ggacaagtgg gcatctctgg
    15541 gctctgggtc tggcaagaac gagcaggagc tgctggagct ggataagtgg gcatccctgg
    15601 ggtccgggtc cggcaagaat gaacaggagc tgctggagct ggataaatgg gcatccctgg
    15661 gctctgggtc tgggaagaat gagcaggagc tgctggaact ggataagtgg gcgagcctgg
    15721 gcagcgggtc tggcaagaat gagcaggaac tgctggagct ggacaagtgg gcttctctgc
    15781 accggtcagc agtggaggac agcacgtaac ccgggcttat atgaagatga agatgaaata
    15841 tttggtgtgt caaataaaaa gcttgtgtgc ttaagtttgt gtttttttct tggcttgttg
    15901 tgttatgaat ttgtggcttt ttctaatatt aaatgaatgt aagatcacat tataatgaat
    15961 aaacaaatgt ttctataatc cattgtgaat gttttgttgg atctcttctg cagcatataa
    16021 ctactgtatg tgctatggta tggactatgg aatatgatta aagataagcg ctgagctcgg
    16081 acggcgcgcc ggagtggctt cgttgaacaa cggaaactcg acttgccttc cgcacaatac
    16141 atcatttctt cttagctttt tttcttcttc ttcgttcata cagttttttt ttgtttatca
    16201 gcttacattt tcttgaaccg tagctttcgt tttcttcttt ttaactttcc attcggagtt
    16261 tttgtatctt gtttcatagt ttgtcccagg attagaatga ttaggcatcg aaccttcaag
    16321 aatttgattg aataaaacat cttcattctt aagatatgaa gataatcttc aaaaggcccc
    16381 tgggaatctg aaagaagaga agcaggccca tttatatggg aaagaacaat agtatttctt
    16441 atataggccc atttaagttg aaaacaatct tcaaaagtcc cacatcgctt agataagaaa
    16501 acgaagctga gtttatatac agctagagtc gaagtagtga ttggatttgc attaactcgg
    16561 gtgttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa
    16621 gtggcaccga gtcggtgctt tttttcccgg tgacgcaatg gtctattggc gttgttgttt
    16681 tccattaggg agcttataga ttattgatta atatgatatt gtggagccac gagtatcatt
    16741 gataagatat aaatttggcc aaaaatgctt ttgattgaaa tatgcaacac cattaagagt
    16801 gtttttgtga aaaaggaatg ctcgtactgc aagctatggt ttcttctatt gtatgacatt
    16861 gccattactt gctatctcta ttgattcatt ttaagcactt tcctgataaa gaatctgttt
    16921 tatccggttg attgctctta tttgtctgtt actgctcaaa ccttacacta tactcccaat
    16981 gtttctatgc cacaggttaa tctaaagagg tgaccgaaag aagaaaactt ttacgttttg
    17041 catggagtaa gaatgccaga gttaagaagt ggagcaagga gatcgagacg ccttgacgag
    17101 cagcctaacc ctccgctagt tgaacaagca gaaaatatcg tacttcctcc tcagactgca
    17161 acaaggagaa gaggtggtgg ctttggcttc gttgaacaac ggaaactcga cttgccttcc
    17221 gcacaataca tcatttcttc ttagcttttt ttcttcttct tcgttcatac agtttttttt
    17281 tgtttatcag cttacatttt cttgaaccgt agctttcgtt ttcttctttt taactttcca
    17341 ttcggagttt ttgtatcttg tttcatagtt tgtcccagga ttagaatgat taggcatcga
    17401 accttcaaga atttgattga ataaaacatc ttcattctta agatatgaag ataatcttca
    17461 aaaggcccct gggaatctga aagaagagaa gcaggcccat ttatatggga aagaacaata
    17521 gtatttctta tataggccca tttaagttga aaacaatctt caaaagtccc acatcgctta
    17581 gataagaaaa cgaagctgag tttatataca gctagagtcg aagtagtgat tgtccagtgt
    17641 attagtgtgg tgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
    17701 cttgaaaaag tggcaccgag tcggtgcttt ttttcccggt gacgccgctg agctcccggg
    17761 gcgcgcccta tggagttcta gaatgtcgcg gaacaaattt taaaactaaa tcctaaattt
    17821 ttctaatttt gttgccaata gtggatatgt gggccgtata gaaggaatct attgaaggcc
    17881 caaacccata ctgacgagcc caaaggttcg ttttgcgttt tatgtttcgg ttcgatgcca
    17941 acgccacatt ctgagctagg caaaaaacaa acgtgtcttt gaatagactc ctctcgttaa
    18001 cacatgcagc ggctgcatgg tgacgccatt aacacgtggc ctacaattgc atgatgtctc
    18061 cattgacacg tgacttctcg tctcctttct taatatatct aacaaacact cctacctctt
    18121 ccaaaatata tacacatctt tttgatcaat ctctcattca aaatctcatt ctctctagta
    18181 aacaagaaca aaaaaatggc ggatacagct agaggaaccc atcacgatat catcggcaga
    18241 gatcagtacc cgatgatggg ccgagatcgt gaccagtacc agatgtccgg acgaggatct
    18301 gactactcca agtctaggca gattgctaaa gctgcaactg ctgtcacagc tggtggttcc
    18361 ctccttgttc tctccagcct tacccttgtt ggaactgtca tagctttgac tgttgcaaca
    18421 cctctgctcg ttatcttcag cccaatcctt gtcccggctc tcatcacagt tgcactcctc
    18481 atcaccggtt ttctttcctc tggagggttt ggcattgccg ctataaccgt tttctcttgg
    18541 atttacaagt aagcacacat ttatcatctt acttcataat tttgtgcaat atgtgcatgc
    18601 atgtgttgag ccagtagctt tggatcaatt tttttggtcg aataacaaat gtaacaataa
    18661 gaaattgcaa attctaggga acatttggtt aactaaatac gaaatttgac ctagctagct
    18721 tgaatgtgtc tgtgtatatc atctatatag gtaaaatgct tggtatgata cctattgatt
    18781 gtgaataggt acgcaacggg agagcaccca cagggatcag acaagttgga cagtgcaagg
    18841 atgaagttgg gaagcaaagc tcaggatctg aaagacagag ctcagtacta cggacagcaa
    18901 catactggtg gggaacatga ccgtgaccgt actcgtggtg gccagcacac tactatgagc
    18961 gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt gaacaaccac
    19021 cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga
    19081 atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc taccagcttc
    19141 atgtacggca gcagaacctt catcaaccac acccagggca tccccgactt ctttaagcag
    19201 tccttccctg agggcttcac atgggagaga gtcaccacat acgaagatgg gggcgtgctg
    19261 accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga
    19321 ggggtgaact tcccatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc
    19381 aacaccgaga tgctgtaccc cgctgacggc ggcctggaag gcagaagcga catggccctg
    19441 aagctcgtgg gcgggggcca cctgatctgc aacttcaaga ccacatacag atccaagaaa
    19501 cccgctaaga acctcaagat gcccggcgtc tactatgtgg accacagact ggaaagaatc
    19561 aaggaggccg acaaagaaac ctacgtcgag cagcacgagg tggctgtggc cagatactgc
    19621 gacctcccta gcaaactggg gcacaagtga gcttacccca ctgatgtcat cgtcatagtc
    19681 caataactcc aatgtcgggg agttagttta tgaggaataa agtgtttaga atttgatcag
    19741 ggggagataa taaaagccga gtttgaatct ttttgttata agtaatgttt atgtgtgttt
    19801 ctatatgttg tcaaatggta ccatgttttt tttcctctct ttttgtaact tgcaagtgtt
    19861 gtgttgtact ttatttggct tctttgtaag ttggtaacgg tggtctatat atggaaaagg
    19921 tcttgttttg ttaaacttat gttagttaac tggattcgtc tttaaccaca aaaagttttc
    19981 aataagctac aaatttagac acgcaagccg atgcagtcat tagtacatat atttattgca
    20041 agtgattaca tggcaaccca aacttcaaaa acagtaggtt gctccattta gtcgctgtta
    20101 gagctctccc ggcgcgccgg aggtcgagct gcaggtcaac ggatcaggat attcttgttt
    20161 aagatgttga actctatgga ggtttgtatg aactgatgat ctaggaccgg ataagttccc
    20221 ttcttcatag cgaacttatt caaagaatgt tttgtgtatc attcttgtta cattgttatt
    20281 aatgaaaaaa tattattggt cattggactg aacacgagtg ttaaatatgg accaggcccc
    20341 aaataagatc cattgatata tgaattaaat aacaagaata aatcgagtca ccaaaccact
    20401 tgcctttttt aacgagactt gttcaccaac ttgatacaaa agtcattatc ctatgcaaat
    20461 caataatcat acaaaaatat ccaataacac taaaaaatta aaagaaatgg ataatttcac
    20521 aatatgttat acgataaaga agttactttt ccaagaaatt cactgatttt ataagcccac
    20581 ttgcattaga taaatggcaa aaaaaaacaa aaaggaaaag aaataaagca cgaagaattc
    20641 tagaaaatac gaaatacgct tcaatgcagt gggacccacg gttcaattat tgccaatttt
    20701 cagctccacc gtatatttaa aaaataaaac gataatgcta aaaaaatata aatcgtaacg
    20761 atcgttaaat ctcaacggct ggatcttatg acgaccgtta gaaattgtgg ttgtcgacga
    20821 gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt gtttatcaac
    20881 tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa aacaactttg
    20941 cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt caccgcctta
    21001 gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa aacaataccc
    21061 aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa aaactttctc
    21121 tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct caaaatcttc
    21181 gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat tctgttaatc
    21241 ttagatcgaa cacgattttc tgggtttgat cgttagatat catcttaatt ctcgattagg
    21301 gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat aattactctt
    21361 cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat cgaatttgtc
    21421 gattaatctg agtttttctg attaacagga atgccaaaga agaagaggaa agtcatggag
    21481 gtgcagctgg tggaatctgg gggcggactg gtgcagcccg ggggatctct gcggctgtcc
    21541 tgcgccgcct ctggctccat ctctagcgtg gatgtgatgt cctggtacag gcaggcccct
    21601 ggcaagcaga gggaactggt ggccttcatt actgataggg gaagaaccaa ttacaaagtg
    21661 agcgtgaaag gccgcttcac tatcagccgg gataattcta agaatatggt gtatctgcag
    21721 atgaacagcc tgaagccaga agacactgcc gactatctgt gcagggctga gtcccggact
    21781 tcctggtcca gcccatctcc tctggatgtg tgggggcgcg gcactcaggt gactgtgtcc
    21841 tccttggatc caggtggagg tggaagcggt agcaaaggag aagaactttt cactggagtt
    21901 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtccgtgga
    21961 gagggtgaag gtgatgctac aaacggaaaa ctcaccctta aatttatttg cactactgga
    22021 aaactacctg ttccgtggcc aacacttgtc actactctga cctatggtgt tcaatgcttt
    22081 tcccgttatc cggatcacat gaaacggcat gactttttca agagtgccat gcccgaaggt
    22141 tatgtacagg aacgcactat atctttcaaa gatgacggga cctacaagac gcgtgctgaa
    22201 gtcaagtttg aaggtgatac ccttgttaat cgtatcgagt taaagggtat tgattttaaa
    22261 gaagatggaa acattcttgg acacaaactc gagtacaact ttaactcaca caatgtatac
    22321 atcacggcag acaaacaaaa gaatggaatc aaagctaact tcaaaattcg ccacaacgtt
    22381 gaagatggtt ccgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
    22441 cctgtccttt taccagacaa ccattacctg tcgacacaat ctgtcctttc gaaagatccc
    22501 aacgaaaagc gtgaccacat ggtccttctt gagtttgtaa ctgctgctgg gattacacat
    22561 ggcatggatg agctctacaa aggtagtggc ggtggtggtt caggaggcgg cggaagctta
    22621 gatccaggtg gaggtggagg tagcaccgtg atgtgggaac aggatgcagc cccctttgca
    22681 ggcgcagccg atgactttcc agcttttaac gaggaggaat tagcgtggct gatggagctg
    22741 ctccctcaaa gcggatctgt gggggggacc attggaggtg gtagtggtgg aggtcggacc
    22801 gaagagtaca agcttatcct gaacggtaaa accctgaaag gtgaaaccac caccgaagct
    22861 gttgacgctg ctaccgcgga aaaagttttc aaacagtacg ctaacgacaa cggtgttgac
    22921 ggtgaatgga cctacgacga cgctaccaaa accttcacgg taaccgaata agcttgttcg
    22981 agtattatgg cattgggaaa actgtttttc ttgtaccatt tgttgtgctt gtaatttact
    23041 gtgtttttta ttcggttttc gctatcgaac tgtgaaatgg aaatggatgg agaagagtta
    23101 atgaatgata tggtcctttt gttcattctc aaattaatat tatttgtttt ttctcttatt
    23161 tgttgtgtgt tgaatttgaa attataagag atatgcaaac attttgtttt gagtaaaaat
    23221 gtgtcaaatc gtggcctcta atgaccgaag ttaatatgag gagtaaaaca cttgtagttg
    23281 tcgctgagct cagtg
//
