LOCUS       sequence-469430-       22770 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA vector for expression of the two protein components of the 
            MoonTag system under the control of the Arabidopsis Ubi10 promoter; 
            Luciferase under control of transactivated promoter by two gRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 22770)
  AUTHORS   Casas-Mollano JA, Zinselmeier MH, Sychla A, Smanski MJ
  TITLE     Efficient gene activation in plants by the MoonTag programmable 
            transcriptional activator.
  JOURNAL   Nucleic Acids Res. 2023 Jul 21;51(13):7083-7093. doi: 
            10.1093/nar/gkad458.
  PUBMED    37254802
REFERENCE   2  (bases 1 to 22770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2023-07-21- 21"; volume: "51"; issue: 
            "13"; pages: "7083-7093"
COMMENT     Sequence Label: pMT1-13X-gRNA1-2(AtUBI10)-MTAP-LUC
FEATURES             Location/Qualifiers
     source          1..22770
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(5..22)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5..21)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14..36)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    224..248
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1549..2178
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2607..3680
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3746..3940
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    4284..4424
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4339..4358
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4439..4456)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4610..5198)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4690..4709)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5285..6079)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    6504..6528
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    6606..6780
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(6837..7634)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6948..6967)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7558..7577
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(7697..8373)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(7740..7760)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    8564..8585
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8600..8605,8606..8623,8624..8630)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8638..8654
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8643..8665
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8662..8678
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8662..8678
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             join(10059..10085,10086..10088,10089..12575,12576..12578,
                     12579..14162)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             10059..14162
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             14166..14192
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             14172..14198
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             14211..14231
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             14238..14258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             14268..14288
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             14355..14399
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             14415..14459
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             14475..14519
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             14535..14579
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             14595..14639
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             14661..14705
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             14721..14765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             14781..14825
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             14841..14885
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             14901..14945
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             14961..15005
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             15021..15065
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             15081..15125
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     terminator      15166..15414
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_RNA        15904..15979
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        16988..17063
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             17563..19215
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(17629..17647)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      19217..19469
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             21220..21930
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             21220..21930
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(21247..21275)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     21870..21890
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             21970..22119
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             22144..22164
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             22195..22356
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             22384..22434
                     /codon_start=1
                     /product="nuclear localization signal from the human T-cell
                     leukemia virus type 1 (HTLV-1) Rex protein"
                     /label=Rex NLS
                     /translation="PKTRRRPRRSQRKRPPT"
ORIGIN
        1 tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
       61 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
      121 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ctagagcagc ttgagcttgg
      181 atcagattgt cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc
      241 gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa
      301 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc
      361 aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
      421 agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc
      481 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg
      541 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg
      601 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg
      661 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg
      721 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc
      781 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg
      841 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt
      901 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga
      961 gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc
     1021 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg
     1081 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg
     1141 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg
     1201 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca
     1261 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc
     1321 cgggtacgtg ttcgagccgc ccgcgcacgg ctcaaccgtg cggctgcatg aaatcctggc
     1381 cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga
     1441 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc
     1501 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc
     1561 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc
     1621 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc
     1681 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg
     1741 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg
     1801 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg
     1861 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag
     1921 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa
     1981 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt
     2041 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt
     2101 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt
     2161 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc
     2221 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag
     2281 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga
     2341 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc
     2401 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga
     2461 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga
     2521 ggaacgggcg gttggccagg cgtaagcggc tgggttgcct gccggccctg caatggcact
     2581 ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa
     2641 tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc
     2701 ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa
     2761 tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca
     2821 agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata
     2881 gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg
     2941 aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca
     3001 tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca
     3061 tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg
     3121 cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag
     3181 aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga
     3241 acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa
     3301 agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg
     3361 agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg
     3421 atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag
     3481 ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt
     3541 tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg
     3601 aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg
     3661 aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg
     3721 aaaaaggtcg aaaaggcctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt
     3781 acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac
     3841 acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa
     3901 aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag
     3961 cccaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt
     4021 cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc
     4081 taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca
     4141 ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggaa
     4201 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     4261 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
     4321 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     4381 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggccctct tccgcttcct
     4441 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     4501 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     4561 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     4621 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     4681 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     4741 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     4801 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     4861 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     4921 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     4981 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     5041 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     5101 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     5161 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     5221 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcattcta
     5281 ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga
     5341 tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg
     5401 accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa
     5461 taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg
     5521 aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat
     5581 ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca
     5641 gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata
     5701 tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc
     5761 tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat
     5821 tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca
     5881 gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt
     5941 ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag
     6001 acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg
     6061 atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc
     6121 ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct
     6181 taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc
     6241 gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc atcgttacaa
     6301 tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt
     6361 cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg
     6421 acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg
     6481 tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt
     6541 agacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc cgaattaatt
     6601 cgggggatct ggattttagt actggatttt ggttttagga attagaaatt ttattgatag
     6661 aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga
     6721 aaccctatag gaaccctaat tcccttatct gggaactact cacacattat tatggagaaa
     6781 ctcgagcttg tcgatcgact ctagctagag gatcgatccg aaccccagag tcccgctcag
     6841 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
     6901 taaagcacga ggaagcggtc agcccattcg ccgccaagtt cttcagcaat atcacgggta
     6961 gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca
     7021 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg tgtcacgacg
     7081 agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc
     7141 ccctgatgtt cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
     7201 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
     7261 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
     7321 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
     7381 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
     7441 gcctcgtcct ggagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
     7501 cgcccctgcg ctgacagccg aaacacggcg gcatcagagc agccgattgt ctgttgtgcc
     7561 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
     7621 tgttcaatcc ccatggtcga tcgacagatc tgcgaaagct cgagagagat agatttgtag
     7681 agagagactg gtgatttcag cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg
     7741 aagggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac
     7801 atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg
     7861 tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc
     7921 ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga
     7981 agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa
     8041 aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga
     8101 gagtgtcgtg ctccaccatg ttcacatcaa tccacttgct ttgaagacgt ggttggaacg
     8161 tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca
     8221 gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct
     8281 tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg
     8341 tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta
     8401 tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttggc aagctgctct
     8461 agccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     8521 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     8581 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     8641 tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat tcgagctcgg
     8701 tacccgggga tcggcgcgcc ggaggtcgag ctgcaggtca acggatcagg atattcttgt
     8761 ttaagatgtt gaactctatg gaggtttgta tgaactgatg atctaggacc ggataagttc
     8821 ccttcttcat agcgaactta ttcaaagaat gttttgtgta tcattcttgt tacattgtta
     8881 ttaatgaaaa aatattattg gtcattggac tgaacacgag tgttaaatat ggaccaggcc
     8941 ccaaataaga tccattgata tatgaattaa ataacaagaa taaatcgagt caccaaacca
     9001 cttgcctttt ttaacgagac ttgttcacca acttgataca aaagtcatta tcctatgcaa
     9061 atcaataatc atacaaaaat atccaataac actaaaaaat taaaagaaat ggataatttc
     9121 acaatatgtt atacgataaa gaagttactt ttccaagaaa ttcactgatt ttataagccc
     9181 acttgcatta gataaatggc aaaaaaaaac aaaaaggaaa agaaataaag cacgaagaat
     9241 tctagaaaat acgaaatacg cttcaatgca gtgggaccca cggttcaatt attgccaatt
     9301 ttcagctcca ccgtatattt aaaaaataaa acgataatgc taaaaaaata taaatcgtaa
     9361 cgatcgttaa atctcaacgg ctggatctta tgacgaccgt tagaaattgt ggttgtcgac
     9421 gagtcagtaa taaacggcgt caaagtggtt gcagccggca cacacgagtc gtgtttatca
     9481 actcaaagca caaatacttt tcctcaacct aaaaataagg caattagcca aaaacaactt
     9541 tgcgtgtaaa caacgctcaa tacacgtgtc attttattat tagctattgc ttcaccgcct
     9601 tagctttctc gtgacctagt cgtcctcgtc ttttcttctt cttcttctat aaaacaatac
     9661 ccaaagagct cttcttcttc acaattcaga tttcaatttc tcaaaatctt aaaaactttc
     9721 tctcaattct ctctaccgtg atcaaggtaa atttctgtgt tccttattct ctcaaaatct
     9781 tcgattttgt tttcgttcga tcccaatttc gtatatgttc tttggtttag attctgttaa
     9841 tcttagatcg aacacgattt tctgggtttg atcgttagat atcatcttaa ttctcgatta
     9901 gggtttcata gatatcatcc gatttgttca aataatttga gttttgtcga ataattactc
     9961 ttcgatttgt gatttctatc tagatctggt gttagtttct agtttgtgcg atcgaatttg
    10021 tcgattaatc tgagtttttc tgattaacag gaatgaacat ggacaagaag tacagcatcg
    10081 gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaggtgc
    10141 ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga
    10201 tcggcgccct gctgttcgac agcggagaaa cagccgaggc cacccggctg aagagaaccg
    10261 ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag atcttcagca
    10321 acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc ttcctggtgg
    10381 aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac gaggtggcct
    10441 accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac agcaccgaca
    10501 aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc cggggccact
    10561 tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg ttcatccagc
    10621 tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc ggcgtggacg
    10681 ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat ctgatcgccc
    10741 agctgcccgg cgagaagaag aatggcctgt tcggcaacct gattgccctg agcctgggcc
    10801 tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg cagctgagca
    10861 aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac cagtacgccg
    10921 acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac atcctgagag
    10981 tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga tacgacgagc
    11041 accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca
    11101 aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacatcgat ggcggagcca
    11161 gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac ggcaccgagg
    11221 aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc ttcgacaacg
    11281 gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg cggcaggaag
    11341 atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg accttccgca
    11401 tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg atgaccagaa
    11461 agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag ggcgccagcg
    11521 cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac gagaaggtgc
    11581 tgcccaagca cagcctgctg tacgagtact tcaccgtgta caacgagctg accaaagtga
    11641 aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag aaaaaagcca
    11701 tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg aaagaggact
    11761 acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa gatcggttca
    11821 acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag gacttcctgg
    11881 acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca ctgtttgagg
    11941 acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac gacaaagtga
    12001 tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg aagctgatca
    12061 acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag tccgacggct
    12121 tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt aaagaggaca
    12181 tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt gccaatctgg
    12241 ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg gacgagctcg
    12301 tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc agagagaacc
    12361 agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc gaagagggca
    12421 tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc cagctgcaga
    12481 acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg gaccaggaac
    12541 tggacatcaa ccggctgtcc gactacgatg tggacgctat cgtgcctcag agctttctga
    12601 aggacgactc catcgataac aaagtgctga ctcggagcga caagaaccgg ggcaagagcg
    12661 acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgc cagctgctga
    12721 atgccaagct gattacccag aggaagttcg acaatctgac caaggccgag agaggcggcc
    12781 tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc cggcagatca
    12841 caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac gagaacgaca
    12901 aactgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga
    12961 aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct
    13021 acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg gaaagcgagt
    13081 tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag agcgagcagg
    13141 aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac tttttcaaga
    13201 ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg
    13261 aaacaggcga gatcgtgtgg gataagggcc gggactttgc caccgtgcgg aaagtgctgt
    13321 ctatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag
    13381 agtctatcct gcccaagagg aacagcgaca agctgatcgc cagaaagaag gactgggacc
    13441 ctaagaagta cggcggcttc gacagcccca ccgtggccta ttctgtgctg gtggtggcca
    13501 aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg gggatcacca
    13561 tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc aagggctaca
    13621 aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc gagctggaaa
    13681 acggccggaa gagaatgctg gcctctgccg gcgaactgca gaagggaaac gaactggccc
    13741 tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag ctgaagggct
    13801 cccccgagga taatgagcag aaacagctgt ttgtggaaca gcacaaacac tacctggacg
    13861 agatcatcga gcagatcagc gagttctcca agagagtgat cctggccgac gctaatctgg
    13921 acaaggtgct gagcgcctac aacaagcaca gagacaagcc tatcagagag caggccgaga
    13981 atatcatcca cctgtttacc ctgaccaatc tgggagcccc tgccgccttc aagtactttg
    14041 acaccaccat cgaccggaag aggtacacca gcaccaaaga ggtgctggac gccaccctga
    14101 tccaccagag catcaccggc ctgtacgaga cacggatcga cctgtctcag ctgggaggcg
    14161 acgcctatcc ctatgacgtg cccgattatg ccagcctggg cagcggctcc cccaagaaaa
    14221 aacgcaaggt ggaagatcct aagaaaaagc ggaaagtgga agatgctcca aagaagaaga
    14281 gaaaggtcga cggcattggt agtgggagca acggcagcag cggatccctt aagcttgcca
    14341 ccatgcgtac gggcaagaat gagcaggaac tgctggaact ggacaaatgg gcaagcctgg
    14401 ggagcggctc cggaaagaac gagcaggaac tgctggaact ggacaagtgg gcttctctgg
    14461 gctccgggtc cgggaaaaac gaacaggagc tgctggagct ggataagtgg gccagcctgg
    14521 gctccgggtc cgggaagaat gaacaggaac tgctggaact ggataaatgg gcatctctgg
    14581 gcagcgggtc cggaaagaat gaacaggagc tgctggaact ggacaagtgg gcatccctgg
    14641 ggagcgggtc cgggtctggg aagaacgagc aggaactgct ggagctggac aagtgggcat
    14701 ctctggggtc tggctctggc aagaacgagc aggaactgct ggaactggac aagtgggcga
    14761 gcctgggcag cgggtctggc aagaatgaac aggagctgct ggaactggac aaatgggcca
    14821 gcctggggtc tgggtctgga aagaatgagc aggaactgct ggagctggac aagtgggcat
    14881 ctctgggctc tgggtctggc aagaacgagc aggagctgct ggagctggat aagtgggcat
    14941 ccctggggtc cgggtccggc aagaatgaac aggagctgct ggagctggat aaatgggcat
    15001 ccctgggctc tgggtctggg aagaatgagc aggagctgct ggaactggat aagtgggcga
    15061 gcctgggcag cgggtctggc aagaatgagc aggaactgct ggagctggac aagtgggctt
    15121 ctctgcaccg gtcagcagtg gaggacagca cgtaacccgg gcttatatga agatgaagat
    15181 gaaatatttg gtgtgtcaaa taaaaagctt gtgtgcttaa gtttgtgttt ttttcttggc
    15241 ttgttgtgtt atgaatttgt ggctttttct aatattaaat gaatgtaaga tcacattata
    15301 atgaataaac aaatgtttct ataatccatt gtgaatgttt tgttggatct cttctgcagc
    15361 atataactac tgtatgtgct atggtatgga ctatggaata tgattaaaga taagcgctga
    15421 gctcggacgg cgcgccggag tggcttcgtt gaacaacgga aactcgactt gccttccgca
    15481 caatacatca tttcttctta gctttttttc ttcttcttcg ttcatacagt ttttttttgt
    15541 ttatcagctt acattttctt gaaccgtagc tttcgttttc ttctttttaa ctttccattc
    15601 ggagtttttg tatcttgttt catagtttgt cccaggatta gaatgattag gcatcgaacc
    15661 ttcaagaatt tgattgaata aaacatcttc attcttaaga tatgaagata atcttcaaaa
    15721 ggcccctggg aatctgaaag aagagaagca ggcccattta tatgggaaag aacaatagta
    15781 tttcttatat aggcccattt aagttgaaaa caatcttcaa aagtcccaca tcgcttagat
    15841 aagaaaacga agctgagttt atatacagct agagtcgaag tagtgattgc tagaaagaag
    15901 aaagttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
    15961 agtggcaccg agtcggtgct ttttttcccg gtgacgcaat ggtctattgg cgttgttgtt
    16021 ttccattagg gagcttatag attattgatt aatatgatat tgtggagcca cgagtatcat
    16081 tgataagata taaatttggc caaaaatgct tttgattgaa atatgcaaca ccattaagag
    16141 tgtttttgtg aaaaaggaat gctcgtactg caagctatgg tttcttctat tgtatgacat
    16201 tgccattact tgctatctct attgattcat tttaagcact ttcctgataa agaatctgtt
    16261 ttatccggtt gattgctctt atttgtctgt tactgctcaa accttacact atactcccaa
    16321 tgtttctatg ccacaggtta atctaaagag gtgaccgaaa gaagaaaact tttacgtttt
    16381 gcatggagta agaatgccag agttaagaag tggagcaagg agatcgagac gccttgacga
    16441 gcagcctaac cctccgctag ttgaacaagc agaaaatatc gtacttcctc ctcagactgc
    16501 aacaaggaga agaggtggtg gctttggctt cgttgaacaa cggaaactcg acttgccttc
    16561 cgcacaatac atcatttctt cttagctttt tttcttcttc ttcgttcata cagttttttt
    16621 ttgtttatca gcttacattt tcttgaaccg tagctttcgt tttcttcttt ttaactttcc
    16681 attcggagtt tttgtatctt gtttcatagt ttgtcccagg attagaatga ttaggcatcg
    16741 aaccttcaag aatttgattg aataaaacat cttcattctt aagatatgaa gataatcttc
    16801 aaaaggcccc tgggaatctg aaagaagaga agcaggccca tttatatggg aaagaacaat
    16861 agtatttctt atataggccc atttaagttg aaaacaatct tcaaaagtcc cacatcgctt
    16921 agataagaaa acgaagctga gtttatatac agctagagtc gaagtagtga ttgtagtatc
    16981 aatggaggtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
    17041 aaaaagtggc accgagtcgg tgcttttttt cccggtgacg ccgctgagct cccggggcgc
    17101 gccggagcaa ttccgagcaa ttaagcgtga cagtcccagt gaacccaccg ctccattgat
    17161 actacctaaa cgtgatcgca gtccatgcaa tcatacgcaa ccgtttcttc tttctagcaa
    17221 aggaagttct ccatacaccg acgcaccagt tggcctctcc attgatacta ctacgccata
    17281 tacataaacg agctgcacaa atgaaaccat ttcttctttc tagcgtgttt gaactggacc
    17341 tctagtttct ctacaaaaac cactccattg atactacata ggttgacctg ttgcgaaatt
    17401 gccttgctta gaccctttct tctttctagc tgcaatgttg tactgatttc ggtccagggt
    17461 aaattcgtaa tttgcattta aaccctagtt aaacgcatta taaaaagacg aaaccctaga
    17521 agctgagagt gagtgagtga gagaagaatc agagaaaatg caatggagga cgccaaaaac
    17581 ataaagaaag gcccggcgcc attctatcct ctagaggatg gaaccgctgg agagcaactg
    17641 cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac agatgcacat
    17701 atcgaggtga acatcacgta cgcggaatac ttcgaaatgt ccgttcggtt ggcagaagct
    17761 atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga aaactctctt
    17821 caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac
    17881 gacatttata atgaacgtga attgctcaac agtatgaaca tttcgcagcc taccgtagtg
    17941 tttgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaaatt accaataatc
    18001 cagaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc gatgtacacg
    18061 ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtacc agagtccttt
    18121 gatcgtgaca aaacaattgc actgataatg aattcctctg gatctactgg gttacctaag
    18181 ggtgtggccc ttccgcatag aactgcctgc gtcagattct cgcatgccag agatcctatt
    18241 tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt ccatcacggt
    18301 tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt cttaatgtat
    18361 agatttgaag aagagctgtt tttacgatcc cttcaggatt acaaaattca aagtgcgttg
    18421 ctagtaccaa ccctattttc attcttcgcc aaaagcactc tgattgacaa atacgattta
    18481 tctaatttac acgaaattgc ttctgggggc gcacctcttt cgaaagaagt cggggaagcg
    18541 gttgcaaaac gcttccatct tccagggata cgacaaggat atgggctcac tgagactaca
    18601 tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg taaagttgtt
    18661 ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg cgttaatcag
    18721 agaggcgaat tatgtgtcag aggacctatg attatgtccg gttatgtaaa caatccggaa
    18781 gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat agcttactgg
    18841 gacgaggacg aacacttctt catagttgac cgcttgaagt ctttaattaa atacaaagga
    18901 tatcaggtgg cccccgctga attggaatcg atattgttac aacaccccaa catcttcgac
    18961 gcgggcgtgg caggtctgcc cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt
    19021 ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta
    19081 acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt
    19141 accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa gaagggcgga
    19201 aagtccaaat tgtaagcttg gactcccatg ttggcaaagg caaccaaaca aacaatgaat
    19261 gatccgctcc tgcatatggg gcggtttgag tatttcaact gccatttggg ctgaattgta
    19321 gacatgctcc tgtcagaaat tccgtgatct tactcaatat tcagtaatct cggccaatat
    19381 cctaaatgtg cgtggcttta tctgtctttg tattgtttca tcaattcatg taacgtttgc
    19441 ttttcttatg aattttcaaa taaattatcc gctgagctct cccggcgcgc cggaggtcga
    19501 gctgcaggtc aacggatcag gatattcttg tttaagatgt tgaactctat ggaggtttgt
    19561 atgaactgat gatctaggac cggataagtt cccttcttca tagcgaactt attcaaagaa
    19621 tgttttgtgt atcattcttg ttacattgtt attaatgaaa aaatattatt ggtcattgga
    19681 ctgaacacga gtgttaaata tggaccaggc cccaaataag atccattgat atatgaatta
    19741 aataacaaga ataaatcgag tcaccaaacc acttgccttt tttaacgaga cttgttcacc
    19801 aacttgatac aaaagtcatt atcctatgca aatcaataat catacaaaaa tatccaataa
    19861 cactaaaaaa ttaaaagaaa tggataattt cacaatatgt tatacgataa agaagttact
    19921 tttccaagaa attcactgat tttataagcc cacttgcatt agataaatgg caaaaaaaaa
    19981 caaaaaggaa aagaaataaa gcacgaagaa ttctagaaaa tacgaaatac gcttcaatgc
    20041 agtgggaccc acggttcaat tattgccaat tttcagctcc accgtatatt taaaaaataa
    20101 aacgataatg ctaaaaaaat ataaatcgta acgatcgtta aatctcaacg gctggatctt
    20161 atgacgaccg ttagaaattg tggttgtcga cgagtcagta ataaacggcg tcaaagtggt
    20221 tgcagccggc acacacgagt cgtgtttatc aactcaaagc acaaatactt ttcctcaacc
    20281 taaaaataag gcaattagcc aaaaacaact ttgcgtgtaa acaacgctca atacacgtgt
    20341 cattttatta ttagctattg cttcaccgcc ttagctttct cgtgacctag tcgtcctcgt
    20401 cttttcttct tcttcttcta taaaacaata cccaaagagc tcttcttctt cacaattcag
    20461 atttcaattt ctcaaaatct taaaaacttt ctctcaattc tctctaccgt gatcaaggta
    20521 aatttctgtg ttccttattc tctcaaaatc ttcgattttg ttttcgttcg atcccaattt
    20581 cgtatatgtt ctttggttta gattctgtta atcttagatc gaacacgatt ttctgggttt
    20641 gatcgttaga tatcatctta attctcgatt agggtttcat agatatcatc cgatttgttc
    20701 aaataatttg agttttgtcg aataattact cttcgatttg tgatttctat ctagatctgg
    20761 tgttagtttc tagtttgtgc gatcgaattt gtcgattaat ctgagttttt ctgattaaca
    20821 ggaatggagg tgcagctggt ggaatctggg ggcggactgg tgcagcccgg gggatctctg
    20881 cggctgtcct gcgccgcctc tggctccatc tctagcgtgg atgtgatgtc ctggtacagg
    20941 caggcccctg gcaagcagag ggaactggtg gccttcatta ctgatagggg aagaaccaat
    21001 tacaaagtga gcgtgaaagg ccgcttcact atcagccggg ataattctaa gaatatggtg
    21061 tatctgcaga tgaacagcct gaagccagaa gacactgccg actatctgtg cagggctgag
    21121 tcccggactt cctggtccag cccatctcct ctggatgtgt gggggcgcgg cactcaggtg
    21181 actgtgtcct ccttggatcc aggtggaggt ggaagcggta gcaaaggaga agaacttttc
    21241 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
    21301 gtccgtggag agggtgaagg tgatgctaca aacggaaaac tcacccttaa atttatttgc
    21361 actactggaa aactacctgt tccgtggcca acacttgtca ctactctgac ctatggtgtt
    21421 caatgctttt cccgttatcc ggatcacatg aaacggcatg actttttcaa gagtgccatg
    21481 cccgaaggtt atgtacagga acgcactata tctttcaaag atgacgggac ctacaagacg
    21541 cgtgctgaag tcaagtttga aggtgatacc cttgttaatc gtatcgagtt aaagggtatt
    21601 gattttaaag aagatggaaa cattcttgga cacaaactcg agtacaactt taactcacac
    21661 aatgtataca tcacggcaga caaacaaaag aatggaatca aagctaactt caaaattcgc
    21721 cacaacgttg aagatggttc cgttcaacta gcagaccatt atcaacaaaa tactccaatt
    21781 ggcgatggcc ctgtcctttt accagacaac cattacctgt cgacacaatc tgtcctttcg
    21841 aaagatccca acgaaaagcg tgaccacatg gtccttcttg agtttgtaac tgctgctggg
    21901 attacacatg gcatggatga gctctacaaa ggtggaggtc ggaccggtcg tacgggcggt
    21961 ggcggagggg atgctttaga cgattttgac ttagatatgc ttggttcaga cgcgttagac
    22021 gacttcgacc tagacatgtt aggctcagat gcattggacg acttcgattt agatatgttg
    22081 ggctccgatg ccctagatga ctttgatcta gatatgctag gtagtggcgg tggcggaggg
    22141 gctccaaaga agaagagaaa ggtccgtacg ggaggaggat ctcggaccga agagtacaag
    22201 cttatcctga acggtaaaac cctgaaaggt gaaaccacca ccgaagctgt tgacgctgct
    22261 accgcggaaa aagttttcaa acagtacgct aacgacaacg gtgttgacgg tgaatggacc
    22321 tacgacgacg ctaccaaaac cttcacggta accgaaggtg gtggtagcgg tggtggtact
    22381 agtccaaaaa caaggaggag gccgcgaaga tcacaacgga aaaggccgcc tacgccatgg
    22441 ccgtaagctt gttcgagtat tatggcattg ggaaaactgt ttttcttgta ccatttgttg
    22501 tgcttgtaat ttactgtgtt ttttattcgg ttttcgctat cgaactgtga aatggaaatg
    22561 gatggagaag agttaatgaa tgatatggtc cttttgttca ttctcaaatt aatattattt
    22621 gttttttctc ttatttgttg tgtgttgaat ttgaaattat aagagatatg caaacatttt
    22681 gttttgagta aaaatgtgtc aaatcgtggc ctctaatgac cgaagttaat atgaggagta
    22741 aaacacttgt agttgtcgct gagctcagtg
//
