LOCUS       sequence-469453-       11449 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Tau-RING protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11449)
  AUTHORS   Miller LVC, Papa G, Vaysburd M, Cheng S, Sweeney PW, Smith A, Franco
            C, Katsinelos T, Huang M, Sanford SAI, Benn J, Farnsworth J, 
            Higginson K, Joyner H, McEwan WA, James LC
  TITLE     Co-opting templated aggregation to degrade pathogenic tau assemblies
            and improve motor function.
  JOURNAL   Cell. 2024 Oct 17;187(21):5967-5980.e17. doi: 
            10.1016/j.cell.2024.08.024. Epub 2024 Sep 13.
  PUBMED    39276772
REFERENCE   2  (bases 1 to 11449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.08.024"; journalName: "Cell"; date: 
            "2024-10-17- 17"; volume: "187"; issue: "21"; pages: "5967-5980.e17"
COMMENT     Sequence Label: pSMPPv2-Tau-RING
FEATURES             Location/Qualifiers
     source          1..11449
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(186..785)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(269..289)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     766..785
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(806..1305)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(854..873)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     1247..1270
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             1337..1348
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1746..1766
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     regulatory      3309..3318
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3375..3782)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    3935..4052
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4337..4381)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4566..4799
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5296..5421
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5468..6101
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6269..6403
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6293..6312)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6347..6366
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6828..6848)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6978..7043
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(8177..8506)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8191..8326
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8491..8511
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(8581..8598)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8752..9340)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8832..8851)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9511..10302,10303..10371))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10134..10153
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10372..10476)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10544..10562
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10600..10622)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10722..10741
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10955..10974
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        11264..11282
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     11264..11281
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             join(11307..11449,1..91)
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 agctcccagg ctcagatctg gtctaaccag agagacccag tacaagcaaa aagcagatct
       61 tgtcttcgtt gggagtgaat tagcccttcc agtcccccct tttcttttaa aaagtggcta
      121 agatctacag ctgccttgta agtcattggt cttaaaggta ccgagctcga attaattccc
      181 tcgagtcagg caccgggctt gcgggtcatg caccaggtgc gcggtccttc gggcacctcg
      241 acgtcggcgg tgacggtgaa gccgagccgc tcgtagaagg ggaggttgcg gggcgcggag
      301 gtctccagga aggcgggcac cccggcgcgc tcggccgcct ccactccggg gagcacgacg
      361 gcgctgccca gacccttgcc ctggtggtcg ggcgagacgc cgacggtggc caggaaccac
      421 gcgggctcct tgggccggtg cggcgccagg aggccttcca tctgttgctg cgcggccagc
      481 cgggaaccgc tcaactcggc catgcgcggg ccgatctcgg cgaacaccgc ccccgcttcg
      541 acgctctccg gcgtggtcca gaccgccacc gcggcgccgt cgtccgcgac ccacaccttg
      601 ccgatgtcga gcccgacgcg cgtgaggaag agttcttgca gctcggtgac ccgctcgatg
      661 tggcggtccg gatcgacggt gtggcgcgtg gcggggtagt cggcgaacgc ggcggcgagg
      721 gtgcgtacgg ccctggggac gtcgtcgcgg gtggcgaggc gcaccgtggg cttgtactcg
      781 gtcatggtaa gcttgggctg caggtcgaaa ggcccggaga tgaggaagag gagaacagcg
      841 cggcagacgt gcgcttttga agcgtgcaga atgccgggcc tccggaggac cttcgggcgc
      901 ccgccccgcc cctgagcccg cccctgagcc cgcccccgga cccacccctt cccagcctct
      961 gagcccagaa agcgaaggag caaagctgct attggccgct gccccaaagg cctacccgct
     1021 tccattgctc agcggtgctg tccatctgca cgagactagt gagacgtgct acttccattt
     1081 gtcacgtcct gcacgacgcg agctgcgggg cgggggggaa cttcctgact aggggaggag
     1141 tagaaggtgg cgcgaagggg ccaccaaaga acggagccgg ttggcgccta ccggtggatg
     1201 tggaatgtgt gcgaggccag aggccacttg tgtagcgcca agtgcccagc ggggctgcta
     1261 aagcgcatgc tccagactgc cttgggaaaa gcgcctcccc tacccggtag aattgatggg
     1321 aaggaaggtc cgctggattg agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa
     1381 tccaggtggc aacacaggcg agcagccaag gaaaggacga tgatttcccc gacaacacca
     1441 cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg caggcggcga
     1501 tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg agctgacagg
     1561 tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac accacgccac
     1621 gttgcctgac aacgggccac aactcctcat aaagagacag caaccaggat ttatacaagg
     1681 aggagaaaat gaaagccata cgggaagcaa tagcatgata caaaggcatt aaagcagcgt
     1741 atccacatag cgtaaaagga gcaacatagt taagaatacc agtcaatctt tcacaaattt
     1801 tgtaatccag aggttgatta tcgataagct tgatatcaag cttgcatgcc tgcaggtcga
     1861 ctctagagtc gcggccgctt actctctggc ctcctggctg atttctttaa ggttgttcac
     1921 catgttggct agctgtcgat tgggccggag attcttgagc agaaagcgct gccggcacac
     1981 aggacagacg ctgcccccac ctttcccaac ctgagagatg cattcctggc agaagctgtg
     2041 gccacactcg atgctcacag gctccacgaa ggggtccagg cagatagggc atgtgacctc
     2101 ctcccacatc attgtcaagc gtgctgctga agccatgcct gagccacctc cggatccgaa
     2161 ttccaaaccc tgcttggcca gggaggcaga cacctcgtca gctagcgtgg cgagctgggg
     2221 cgagtctacc atgtcgatgc tgccggtgga ggagacattg ctgagatgcc gtggagacgt
     2281 gtccccagac accactggcg acttgtacac gatctccgcc ccgtggtctg tcttggcttt
     2341 ggcgttctcg cggaaggtca gcttgtgggt ttcaatcttt ttatttcctc cgccagggac
     2401 gtgggtgata ttgtccaggg acccaatctt cgactggact ctgtccttga agtcaagctt
     2461 ctcagatttt acttccacct ggccacctcc tggtttatga tggatgttgc ctaatgagcc
     2521 acacttggag gtcaccttgc tcaggtcaac tggtttgtag actatttgca cactgccgcc
     2581 tccactgacg tgtttgatat tatcctttga gccacacttg gactggacgt tgctaagatc
     2641 cagcttctta ttaattatct gcaccttccc gcctcccggc tggtgcttca ggttctcagt
     2701 ggagccgatc ttggacttga cattcttcag gtctggcatg ggcacggggg ctgtctgcag
     2761 gcggctcttg gcggaagacg gcgacttggg tggagtacgg accactgcca ccttcttggg
     2821 ctcccgggtg ggtggggttg gaagggacgg ggtgcgggag cggctgccgg gagtgcctgg
     2881 ggagccgggg ctgctgtagc cgctgcgatc ccctgatttt ggaggttcac cagagctggg
     2941 tggtgtcttt ggagcgggcg gggtttttgc tggaatcctg gtggcgttgg cctggccctt
     3001 ctggcctgga ggggctgctc cccgcggtgt ggcgatcttc gttttaccat cagccccctt
     3061 ggcttttttg tcatcgcttc cagtcccgtc tttgctttta ctgaccatgc gagcttgggt
     3121 cacgtgacca gcagcttcgt cttccaggct gggggtgtct ccaatgcctg cttcttcagc
     3181 tttcaggcca gcgtccgtgt caccctcttg gtcttggtgc atggtgtagc ccccctgatc
     3241 tttcctgtcc cccaacccgt acgtcccagc gtgatcttcc atcacttcga actcctggcg
     3301 gggctcagcc atggtggcac gcgtatcctg cagcaggtgt ccgtcaactg tcacctgccg
     3361 atcagttgat cccccccggg cgactcagtc tgtcggagga ctggcgcgcc gagtgagggg
     3421 ttgtgagctc ttttatagag ctcgggaagc agaagcgcgc gaacagaagc gagaagcagg
     3481 ctgattggtt aattcaaata aggcgcaggg tcatttcagg tccttggggg agcctggaaa
     3541 catctgatgg gtcttaagaa actgctgagg gttgggccat atctggggac catctgttct
     3601 tggcctcggg ccggggccga aactgcggtg accatctgtt cttggccccg ggccggggcc
     3661 gaaactgctc accgcagata tcctgtttgg cccaacgtta gctattttca tgtacccgcc
     3721 cttgatctga acttctctat tcttggtttg gtatttttcc atgccttgca aaatggcgtt
     3781 actgcagcta gcttgccaaa cctacaggtg gggtctttca ttcccccctt tttctggaga
     3841 ctaaataaaa tcttttattt tatcggggct gcaggaattc gatatcaagc ttatcgatcc
     3901 aaactggatc tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt gtaatttgtt
     3961 tttgtaattc tttagtttgt atgtctgttg ctattatgtc tactattctt tcccctgcac
     4021 tgtacccccc aatcccccct tttcttttaa aattgtggat gaatactgcc atttggcgat
     4081 accgtcgaga tccgttcact aatcgaatgg atctgtctct gtctctctct ccaccttctt
     4141 cttctattcc ttcgggcctg tcgggtcccc tcggggttgg gaggtgggtc tgaaacgata
     4201 atggtgaata tccctgccta actctattca ctatagaaag tacagcaaaa actattctta
     4261 aacctaccaa gcctcctact atcattatga ataattttat ataccacagc caatttgtta
     4321 tgttaaacca attccacaaa cttgcccatt tatctaattc caataattct tgttcattct
     4381 tttcttgctg gttttgcgat tcttcaatta aggagtgtat taagcttgtg taattgttaa
     4441 tttctctgtc ccactccatc caggtcgtgt gattccaaat ctgttccaga gatttattac
     4501 tccaactagc attccaaggc acagcagtgg tgcaaatgag ttttccagag caaccccaaa
     4561 tccccaggag ctgttgatcc tttaggtatc tttccacagc caggattctt gcctggagct
     4621 gcttgatgcc ccagactgtg agttgcaaca gatgctgttg cgcctcaata gccctcagca
     4681 aattgttctg ctgctgcact ataccagaca ataattgtct ggcctgtacc gtcagcgtca
     4741 ttgacgctgc gcccatagtg cttcctgctg ctcccaagaa cccaaggaac aaagctccta
     4801 ttcccactgc tcttttttct ctctgcacca ctcttctctt tgccttggtg ggtgctactc
     4861 ctaatggttc aatttttact actttatatt tatataattc acttctccaa ttgtccctca
     4921 tatctcctcc tccaggtctg aagatcagcg gccggccgct tgctgtgcgg tggtcttact
     4981 tttgttttgc tcttcctcta tcttgtctaa agcttccttg gtgtctttta tctctatcct
     5041 ttgatgcaca caatagaggg ttgctactgt attatataat gatctaagtt cttctgatcc
     5101 tgtctgaagg gatggttgta gctgtcccag tatttgtcta cagccttctg atgtttctaa
     5161 caggccagga ttaactgcga atcgttctag ctccctgctt gcccatacta tatgttttaa
     5221 tttatatttt ttctttcccc ctggccttaa ccgaattttt tcccatcgcg atctaattct
     5281 cccccgctta atactgacgc tctcgcaccc atctctctcc ttctagcctc cgctagtcaa
     5341 aatttttggc gtactcacca gtcgccgccc ctcgcctctt gccgtgcgcg cttcagcaag
     5401 ccgagtcctg cgtcgagaga gctcctctgg tttccctttc gctttcaagt ccctgttcgg
     5461 gcgccactgc tagagatttt ccacactgac taaaagggtc tgagggatct ctagttacca
     5521 gagtcacaca acagacgggc acacactact tgaagcactc aaggcaagct ttattgaggc
     5581 ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg gtctaaccag
     5641 agagacccag tacaggcaaa aagcagctgc ttatatgcag gatctgaggg ctcgccactc
     5701 cccagtcccg cccaggccac gcctccctgg aaagtcccca gcggaaagtc ccttgtagca
     5761 agctcgatat cagcagttct tgaagtactc cggatgcagc tctcgggcca cgtgatgaaa
     5821 tgctaggcgg ctgtcaaacc tccactctaa cacttctctc tccgggtcat ccatcccatg
     5881 caggctcaca gggtgtaaca agctggtgtt ctctccttta ttggcctctt ctaccttatc
     5941 tggctcaact ggtactagct tgtagcacca tccaaaggtc agtggatatc tgacccctgg
     6001 ccctggtgtg tagttctgct aatcagggaa gtagccttgt gtgtggtaga tccacagatc
     6061 aaggatatct tgtcttcttt gggagtgaat tagcccttcc aactactaag tttgtagtac
     6121 atatttaaca aatacaattt ctttaaaatg aaaataattc agaggaatca caggtttaga
     6181 gtaaatgaaa ccacaggtaa ttggcagtgg taatagggta tggggtggga agtttgggat
     6241 gattttggtt agcttgagtt atccagttga tccagacatg ataagataca ttgatgagtt
     6301 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     6361 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     6421 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     6481 ctacaaatgt ggtatggctg attatgatct ctagtcaagg cactatacat caaatattcc
     6541 ttattaaccc ctttacaaat taaaaagcta aaggtacaca atttttgagc atagttatta
     6601 atagcagaca ctctatgcct gtgtggagta agaaaaaaca gtatgttatg attataactg
     6661 ttatgcctac ttataaaggt tacagaatat ttttccataa ttttcttgta tagcagtgca
     6721 gctttttcct ttgtggtgta aatagcaaag caagcaagag ttctattact aaacacagca
     6781 tgactcaaaa aacttagcaa ttctgaagga aagtccttgg ggtcttctac ctttctcttc
     6841 ttttttggag gagtagaatg ttgagagtca gcagtagcct catcatcact agatggcatt
     6901 tcttctgagc aaaacaggtt ttcctcatta aaggcattcc accactgctc ccattcatca
     6961 gttccatagg ttggaatcta aaatacacaa acaattagaa tcagtagttt aacacattat
     7021 acacttaaaa attttatatt taccttagag ctttaaatct ctgtaggtag tttgtccaat
     7081 tatgtcacac cacagaagta aggttccttc acaaagatcc ggggcccact cataaatcca
     7141 gttgccgcca cggtagccaa tcaccgtatc gtataaatca tcgtcggtac gttcggcatc
     7201 gctcatcaca atacgtgcct ggacgtcgag gatttcgcgt gggtcaatgc cgcgccagat
     7261 ccacatcaga cggttaatca tgcgatacca gtgagggatg gttttaccat caagggccga
     7321 ctgcacaggc ggttgtgcgc cgtgattaaa gcggcggact agcgtcgagg tttcaggatg
     7381 tttaaagcgg ggtttgaaca gggtttcgct caggtttgcc tgtgtcatgg atgcagcctc
     7441 cagaatactt actggaaact attgtaaccc gcctgaagtt aaaaagaaca acgcccggca
     7501 gtgccaggcg ttgaaaagat tagcgaccgg agattggcgg gacgaatacg acgcccatat
     7561 cccacggctg ttcaatccag gtatcttgcg ggatatcaac aacatagtca tcaaccagcg
     7621 gacgaccagc cggttttgcg aagatggtga caaagtgcgc ttttggatac atttcacgaa
     7681 tcgcaaccgc agtaccaccg gtatccacca ggtcatcaat aacgatgaag ccttcgccat
     7741 cgccttctgc gcgtttcagc actttaagct cgcgctggtt gtcgtgatcg tagctggaaa
     7801 tacaaacggt atcgacatga cgaataccca gttcacgcgc cagtaacgca cccggtacag
     7861 accgccacgg cttacggcaa taatgccttt ccattgttca gaaggcatca gtcggcttgc
     7921 gagtttacgt gcatggatct gcaacatgtc ccaggtgacg atgtattttt cgctcatgtg
     7981 aagtgtccca gcctgtttat ctacggctta aaaagtgttc gaggggaaaa taggttgcgc
     8041 gagattatag agatctggcg cactaaaaac cagtatttca catgagtccg cgtcttttta
     8101 cgcactgcct ctccctgacg cgggataaag tggtattctc aaacatatct cgcaagcctg
     8161 tcttgtgtcc aagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct
     8221 ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc
     8281 catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg
     8341 gcgggactat ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg
     8401 agcctgggga ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc
     8461 tgcctgctgg ggagcctggg gactttccac accctaactg acacacattc cacagctgca
     8521 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     8581 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     8641 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     8701 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     8761 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     8821 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     8881 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     8941 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     9001 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     9061 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     9121 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     9181 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     9241 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     9301 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     9361 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     9421 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     9481 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     9541 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     9601 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     9661 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9721 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9781 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     9841 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9901 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9961 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    10021 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    10081 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    10141 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    10201 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    10261 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    10321 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    10381 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    10441 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    10501 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
    10561 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
    10621 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
    10681 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
    10741 actgagagtg caccattcga cgctctccct tatgcgactc ctgcattagg aagcagccca
    10801 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg
    10861 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
    10921 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
    10981 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatctggc
    11041 tagcgatgac cctgctgatt ggttcgctga ccatttccgg gtgcgggacg gcgttaccag
    11101 aaactcagaa ggttcgtcca accaaaccga ctctgacggc agtttacgag agagatgata
    11161 gggtctgctt cagtaagcca gatgctacac aattaggctt gtacatattg tcgttagaac
    11221 gcggctacaa ttaatacata accttatgta tcatacacat acgatttagg tgacactata
    11281 gaatacacgg aattaattct agatgctgct agagattttc cacactgact aaaagggtct
    11341 gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca
    11401 aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagag
//
