LOCUS       sequence-469468-       11428 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Tau-RING I18R-M72E mutant in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11428)
  AUTHORS   Miller LVC, Papa G, Vaysburd M, Cheng S, Sweeney PW, Smith A, Franco
            C, Katsinelos T, Huang M, Sanford SAI, Benn J, Farnsworth J, 
            Higginson K, Joyner H, McEwan WA, James LC
  TITLE     Co-opting templated aggregation to degrade pathogenic tau assemblies
            and improve motor function.
  JOURNAL   Cell. 2024 Oct 17;187(21):5967-5980.e17. doi: 
            10.1016/j.cell.2024.08.024. Epub 2024 Sep 13.
  PUBMED    39276772
REFERENCE   2  (bases 1 to 11428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.08.024"; journalName: "Cell"; date: 
            "2024-10-17- 17"; volume: "187"; issue: "21"; pages: "5967-5980.e17"
COMMENT     Sequence Label: pSMPPv2-Tau-RING (M72E/I18R)
FEATURES             Location/Qualifiers
     source          1..11428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     21..40
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(259..363)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     431..449
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(487..509)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     609..628
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     842..861
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        1151..1169
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1151..1168
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             1194..1427
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(1522..2121)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1605..1625)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2102..2121
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(2142..2641)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2190..2209)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     2583..2606
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             2673..2684
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3082..3102
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     regulatory      4645..4654
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4690..5097)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    5250..5367
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5652..5696)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5881..6114
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6611..6736
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6783..7416
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    7584..7718
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7608..7627)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7662..7681
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(8143..8163)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          8293..8358
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(9492..9821)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9506..9641
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9806..9826
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(9896..9913)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10067..10655)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10147..10166)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10826..11428,1..189,190..258))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
       61 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
      121 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
      181 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
      241 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
      301 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
      361 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
      421 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
      481 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
      541 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
      601 taactatgcg gcatcagagc agattgtact gagagtgcac cattcgacgc tctcccttat
      661 gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg
      721 caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca
      781 ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat
      841 cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca
      901 cgatgcgtcc ggcgtagagg atctggctag cgatgaccct gctgattggt tcgctgacca
      961 tttccgggtg cgggacggcg ttaccagaaa ctcagaaggt tcgtccaacc aaaccgactc
     1021 tgacggcagt ttacgagaga gatgataggg tctgcttcag taagccagat gctacacaat
     1081 taggcttgta catattgtcg ttagaacgcg gctacaatta atacataacc ttatgtatca
     1141 tacacatacg atttaggtga cactatagaa tacacggaat taattctaga tgctgctaga
     1201 gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag
     1261 acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt
     1321 ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca
     1381 agcaaaaagc agatcttgtc ttcgttggga gtgaattagc ccttccagtc cccccttttc
     1441 ttttaaaaag tggctaagat ctacagctgc cttgtaagtc attggtctta aaggtaccga
     1501 gctcgaatta attccctcga gtcaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg
     1561 tccttcgggc acctcgacgt cggcggtgac ggtgaagccg agccgctcgt agaaggggag
     1621 gttgcggggc gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac
     1681 tccggggagc acgacggcgc tgcccagacc cttgccctgg tggtcgggcg agacgccgac
     1741 ggtggccagg aaccacgcgg gctccttggg ccggtgcggc gccaggaggc cttccatctg
     1801 ttgctgcgcg gccagccggg aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa
     1861 caccgccccc gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc
     1921 cgcgacccac accttgccga tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc
     1981 ggtgacccgc tcgatgtggc ggtccggatc gacggtgtgg cgcgtggcgg ggtagtcggc
     2041 gaacgcggcg gcgagggtgc gtacggccct ggggacgtcg tcgcgggtgg cgaggcgcac
     2101 cgtgggcttg tactcggtca tggtaagctt gggctgcagg tcgaaaggcc cggagatgag
     2161 gaagaggaga acagcgcggc agacgtgcgc ttttgaagcg tgcagaatgc cgggcctccg
     2221 gaggaccttc gggcgcccgc cccgcccctg agcccgcccc tgagcccgcc cccggaccca
     2281 ccccttccca gcctctgagc ccagaaagcg aaggagcaaa gctgctattg gccgctgccc
     2341 caaaggccta cccgcttcca ttgctcagcg gtgctgtcca tctgcacgag actagtgaga
     2401 cgtgctactt ccatttgtca cgtcctgcac gacgcgagct gcggggcggg ggggaacttc
     2461 ctgactaggg gaggagtaga aggtggcgcg aaggggccac caaagaacgg agccggttgg
     2521 cgcctaccgg tggatgtgga atgtgtgcga ggccagaggc cacttgtgta gcgccaagtg
     2581 cccagcgggg ctgctaaagc gcatgctcca gactgccttg ggaaaagcgc ctcccctacc
     2641 cggtagaatt gatgggaagg aaggtccgct ggattgaggg ccgaagggac gtagcagaag
     2701 gacgtcccgc gcagaatcca ggtggcaaca caggcgagca gccaaggaaa ggacgatgat
     2761 ttccccgaca acaccacgga attgtcagtg cccaacagcc gagcccctgt ccagcagcgg
     2821 gcaaggcagg cggcgatgag ttccgccgtg gcaataggga gggggaaagc gaaagtcccg
     2881 gaaaggagct gacaggtggt ggcaatgccc caaccagtgg gggttgcgtc agcaaacaca
     2941 gtgcacacca cgccacgttg cctgacaacg ggccacaact cctcataaag agacagcaac
     3001 caggatttat acaaggagga gaaaatgaaa gccatacggg aagcaatagc atgatacaaa
     3061 ggcattaaag cagcgtatcc acatagcgta aaaggagcaa catagttaag aataccagtc
     3121 aatctttcac aaattttgta atccagaggt tgattatcga taagcttgat atcaagcttg
     3181 catgcctgca ggtcgagtcg acggagccga tatcttactc tctggcctct tgggagatct
     3241 ctttcaggtt gttgacctcg ttggccagct gccggttagg ccgcaggttc ttcagcagga
     3301 atctctgtct gcacacaggg cacacagatc cgccgccttt gccgacttgg ctgatgcact
     3361 cttggcagaa gctgtggccg cattcgatgg acacgggttc cacgaagggg tccagacacc
     3421 taggacaggt cacttcttcc cacatcatgg tcagtctggc ggcagaggcc atcgatccgc
     3481 caccaccgga gccgaattcc aaaccctgct tggccaggga ggcagacacc tcgtcagcta
     3541 gcgtggcgag ctggggcgag tctaccatgt cgatgctgcc ggtggaggag acattgctga
     3601 gatgccgtgg agacgtgtcc ccagacacca ctggcgactt gtacacgatc tccgccccgt
     3661 ggtctgtctt ggctttggcg ttctcgcgga aggtcagctt gtgggtttca atctttttat
     3721 ttcctccgcc agggacgtgg gtgatattgt ccagggaccc aatcttcgac tggactctgt
     3781 ccttgaagtc aagcttctca gattttactt ccacctggcc acctcctggt ttatgatgga
     3841 tgttgcctaa tgagccacac ttagaggtca ccttgctcag gtcaactggt ttgtagacta
     3901 tttgcacact gccgcctcca ctgacgtgtt tgatattatc ctttgatcca cacttggact
     3961 ggacgttgct aagatccagc ttcttattaa ttatctgcac cttcccgcct cccggctggt
     4021 gcttcaggtt ctcagtggag ccgatcttgg acttgacatt cttcaggtct ggcatgggca
     4081 cgggggctgt ctgcaggcgg ctcttggcgg aagacggcga cttgggtgga gtacggacca
     4141 ctgccacctt cttgggctcc cgggtgggtg gggttggaag ggacggggta cgggagcggc
     4201 tgccgggagt gcctggggag ccggggctgc tgtagccgct gcgatcccct gattttggag
     4261 gttcaccaga gctgggtggt gtctttggag cgggcggggt ttttgctgga atcctggtgg
     4321 cgttggcctg gcccttctgg cctggagggg ctgctccccg cggtgtggcg atcttcgttt
     4381 taccatcagc ccccttggct tttttgtcat cgcttccagt cccgtctttg cttttactga
     4441 ccatgcgagc ttgggtcacg tgaccagcag cttcgtcttc caggctgggg gtgtctccaa
     4501 tgcctgcttc ttcagctttc aggccagcgt ccgtgtcacc ctcttggtct tggtgcatgg
     4561 tgtagccccc ctgatctttc ctgtccccca acccgtacgt cccagcgtga tcttccatca
     4621 cttcgaactc ctggcggggc tcagccatgg tggcgaattc acgcgttctg atgatcacag
     4681 tggatccccc ccgggcgact cagtctgtcg gaggactggc gcgccgagtg aggggttgtg
     4741 agctctttta tagagctcgg gaagcagaag cgcgcgaaca gaagcgagaa gcaggctgat
     4801 tggttaattc aaataaggcg cagggtcatt tcaggtcctt gggggagcct ggaaacatct
     4861 gatgggtctt aagaaactgc tgagggttgg gccatatctg gggaccatct gttcttggcc
     4921 tcgggccggg gccgaaactg cggtgaccat ctgttcttgg ccccgggccg gggccgaaac
     4981 tgctcaccgc agatatcctg tttggcccaa cgttagctat tttcatgtac ccgcccttga
     5041 tctgaacttc tctattcttg gtttggtatt tttccatgcc ttgcaaaatg gcgttactgc
     5101 agctagcttg ccaaacctac aggtggggtc tttcattccc ccctttttct ggagactaaa
     5161 taaaatcttt tattttatcg gggctgcagg aattcgatat caagcttatc gatccaaact
     5221 ggatctctgc tgtccctgta ataaacccga aaattttgaa tttttgtaat ttgtttttgt
     5281 aattctttag tttgtatgtc tgttgctatt atgtctacta ttctttcccc tgcactgtac
     5341 cccccaatcc ccccttttct tttaaaattg tggatgaata ctgccatttg gcgataccgt
     5401 cgagatccgt tcactaatcg aatggatctg tctctgtctc tctctccacc ttcttcttct
     5461 attccttcgg gcctgtcggg tcccctcggg gttgggaggt gggtctgaaa cgataatggt
     5521 gaatatccct gcctaactct attcactata gaaagtacag caaaaactat tcttaaacct
     5581 accaagcctc ctactatcat tatgaataat tttatatacc acagccaatt tgttatgtta
     5641 aaccaattcc acaaacttgc ccatttatct aattccaata attcttgttc attcttttct
     5701 tgctggtttt gcgattcttc aattaaggag tgtattaagc ttgtgtaatt gttaatttct
     5761 ctgtcccact ccatccaggt cgtgtgattc caaatctgtt ccagagattt attactccaa
     5821 ctagcattcc aaggcacagc agtggtgcaa atgagttttc cagagcaacc ccaaatcccc
     5881 aggagctgtt gatcctttag gtatctttcc acagccagga ttcttgcctg gagctgcttg
     5941 atgccccaga ctgtgagttg caacagatgc tgttgcgcct caatagccct cagcaaattg
     6001 ttctgctgct gcactatacc agacaataat tgtctggcct gtaccgtcag cgtcattgac
     6061 gctgcgccca tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc tcctattccc
     6121 actgctcttt tttctctctg caccactctt ctctttgcct tggtgggtgc tactcctaat
     6181 ggttcaattt ttactacttt atatttatat aattcacttc tccaattgtc cctcatatct
     6241 cctcctccag gtctgaagat cagcggccgg ccgcttgctg tgcggtggtc ttacttttgt
     6301 tttgctcttc ctctatcttg tctaaagctt ccttggtgtc ttttatctct atcctttgat
     6361 gcacacaata gagggttgct actgtattat ataatgatct aagttcttct gatcctgtct
     6421 gaagggatgg ttgtagctgt cccagtattt gtctacagcc ttctgatgtt tctaacaggc
     6481 caggattaac tgcgaatcgt tctagctccc tgcttgccca tactatatgt tttaatttat
     6541 attttttctt tccccctggc cttaaccgaa ttttttccca tcgcgatcta attctccccc
     6601 gcttaatact gacgctctcg cacccatctc tctccttcta gcctccgcta gtcaaaattt
     6661 ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag
     6721 tcctgcgtcg agagagctcc tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc
     6781 actgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc
     6841 acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag
     6901 cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga
     6961 cccagtacag gcaaaaagca gctgcttata tgcaggatct gagggctcgc cactccccag
     7021 tcccgcccag gccacgcctc cctggaaagt ccccagcgga aagtcccttg tagcaagctc
     7081 gatatcagca gttcttgaag tactccggat gcagctctcg ggccacgtga tgaaatgcta
     7141 ggcggctgtc aaacctccac tctaacactt ctctctccgg gtcatccatc ccatgcaggc
     7201 tcacagggtg taacaagctg gtgttctctc ctttattggc ctcttctacc ttatctggct
     7261 caactggtac tagcttgtag caccatccaa aggtcagtgg atatctgacc cctggccctg
     7321 gtgtgtagtt ctgctaatca gggaagtagc cttgtgtgtg gtagatccac agatcaagga
     7381 tatcttgtct tctttgggag tgaattagcc cttccaacta ctaagtttgt agtacatatt
     7441 taacaaatac aatttcttta aaatgaaaat aattcagagg aatcacaggt ttagagtaaa
     7501 tgaaaccaca ggtaattggc agtggtaata gggtatgggg tgggaagttt gggatgattt
     7561 tggttagctt gagttatcca gttgatccag acatgataag atacattgat gagtttggac
     7621 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     7681 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     7741 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     7801 aatgtggtat ggctgattat gatctctagt caaggcacta tacatcaaat attccttatt
     7861 aaccccttta caaattaaaa agctaaaggt acacaatttt tgagcatagt tattaatagc
     7921 agacactcta tgcctgtgtg gagtaagaaa aaacagtatg ttatgattat aactgttatg
     7981 cctacttata aaggttacag aatatttttc cataattttc ttgtatagca gtgcagcttt
     8041 ttcctttgtg gtgtaaatag caaagcaagc aagagttcta ttactaaaca cagcatgact
     8101 caaaaaactt agcaattctg aaggaaagtc cttggggtct tctacctttc tcttcttttt
     8161 tggaggagta gaatgttgag agtcagcagt agcctcatca tcactagatg gcatttcttc
     8221 tgagcaaaac aggttttcct cattaaaggc attccaccac tgctcccatt catcagttcc
     8281 ataggttgga atctaaaata cacaaacaat tagaatcagt agtttaacac attatacact
     8341 taaaaatttt atatttacct tagagcttta aatctctgta ggtagtttgt ccaattatgt
     8401 cacaccacag aagtaaggtt ccttcacaaa gatccggggc ccactcataa atccagttgc
     8461 cgccacggta gccaatcacc gtatcgtata aatcatcgtc ggtacgttcg gcatcgctca
     8521 tcacaatacg tgcctggacg tcgaggattt cgcgtgggtc aatgccgcgc cagatccaca
     8581 tcagacggtt aatcatgcga taccagtgag ggatggtttt accatcaagg gccgactgca
     8641 caggcggttg tgcgccgtga ttaaagcggc ggactagcgt cgaggtttca ggatgtttaa
     8701 agcggggttt gaacagggtt tcgctcaggt ttgcctgtgt catggatgca gcctccagaa
     8761 tacttactgg aaactattgt aacccgcctg aagttaaaaa gaacaacgcc cggcagtgcc
     8821 aggcgttgaa aagattagcg accggagatt ggcgggacga atacgacgcc catatcccac
     8881 ggctgttcaa tccaggtatc ttgcgggata tcaacaacat agtcatcaac cagcggacga
     8941 ccagccggtt ttgcgaagat ggtgacaaag tgcgcttttg gatacatttc acgaatcgca
     9001 accgcagtac caccggtatc caccaggtca tcaataacga tgaagccttc gccatcgcct
     9061 tctgcgcgtt tcagcacttt aagctcgcgc tggttgtcgt gatcgtagct ggaaatacaa
     9121 acggtatcga catgacgaat acccagttca cgcgccagta acgcacccgg tacagaccgc
     9181 cacggcttac ggcaataatg cctttccatt gttcagaagg catcagtcgg cttgcgagtt
     9241 tacgtgcatg gatctgcaac atgtcccagg tgacgatgta tttttcgctc atgtgaagtg
     9301 tcccagcctg tttatctacg gcttaaaaag tgttcgaggg gaaaataggt tgcgcgagat
     9361 tatagagatc tggcgcacta aaaaccagta tttcacatga gtccgcgtct ttttacgcac
     9421 tgcctctccc tgacgcggga taaagtggta ttctcaaaca tatctcgcaa gcctgtcttg
     9481 tgtccaagct ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaat
     9541 agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg
     9601 ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg
     9661 actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct
     9721 ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat acttctgcct
     9781 gctggggagc ctggggactt tccacaccct aactgacaca cattccacag ctgcattaat
     9841 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     9901 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     9961 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
    10021 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
    10081 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
    10141 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
    10201 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
    10261 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
    10321 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    10381 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    10441 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    10501 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    10561 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    10621 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    10681 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    10741 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    10801 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    10861 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    10921 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    10981 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    11041 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    11101 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    11161 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    11221 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    11281 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    11341 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    11401 aatagtgtat gcggcgaccg agttgctc
//
