LOCUS       sequence-46948-e        5974 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Kieback E, Muller M
  TITLE     Factors influencing subcellular localization of the human 
            papillomavirus L2 minor structural protein.
  JOURNAL   Virology. 2006 Feb 5;345(1):199-208. Epub 2005 Oct 27.
  PUBMED    16257028
REFERENCE   2  (bases 1 to 5974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2006-02-5"; volume: "345(1)"; pages: "199-208. Epub 2005 Oct 
            27"
COMMENT     Sequence Label: p11L2w
FEATURES             Location/Qualifiers
     source          1..5974
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        113..1291
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          344..1282
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1239..1259
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    2791..3379
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2844..2864)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3262..3273)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3436..3557
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3447..3466)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3501..3520
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3745..4333
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4234..4253
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(4417..4437)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4422..4751
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4602..4737
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4664..4683
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4799..4846
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        4838..4856
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4866..5660
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4866..5660
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4920..4939)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5530..5549
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5718..5737)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5762..5809
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ccgctaccgg actcagatct cgagctcaag cttgcaaaga tggataaagt tttaaacaga
       61 gaggaatctt tgcagctaat ggaccttgta ggtcttgaaa ggagtgggaa ttggctccgg
      121 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
      181 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
      241 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
      301 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
      361 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
      421 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
      481 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
      541 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
      601 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
      661 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
      721 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
      781 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
      841 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
      901 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
      961 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
     1021 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
     1081 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
     1141 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
     1201 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
     1261 aagttttttt cttccatttc aggtgtcgtg aggaattctc tagagatccc tcgacctcga
     1321 gatccatcct gtacggaagt gttacttctg ctctaaaagc tgcggaattg tacccgcggc
     1381 cgctctagat gaagcccaga gcccggcgga gaaagcgggc cagcgccacc cagctgtacc
     1441 agacctgcaa ggccaccggc acctgccccc ctgacgtgat ccccaaggtg gagcacacca
     1501 ccatcgccga ccagatcctg aagtggggca gcctgggcgt gttcttcggc ggcctgggca
     1561 tcggcaccgg cgccggcagc ggcggccggg ccggctacat ccccctgggc agcagcccta
     1621 agcccgccat caccgggggc cctgccgcca gaccccccgt gctggtggag cctgtggccc
     1681 ccagcgaccc cagcatcgtg agtctgatcg aggagagcgc catcatcaac gccggcgccc
     1741 ctgaggtggt gcctcctacc cagggcggct tcaccatcac cagcagcgag agcaccaccc
     1801 ctgccatcct ggacgtgagc gtgaccaacc acaccaccac cagcgtgttc cagaaccccc
     1861 tgttcaccga gcctagcgtg atccagcccc agcctcccgt ggaggccagc ggccacatcc
     1921 tgatcagcgc ccctaccatc accagccagc acgtggagga catccccctg gacaccttcg
     1981 tggtgagcag cagcgacagc ggccccacca gcagcacccc tctgccccgg gccttcccca
     2041 gacctcgggt gggcctgtac agcagagccc tgcagcaggt gcaggtgacc gaccccgcct
     2101 tcctgagcac cccccagcgg ctggtgacct acgacaaccc tgtgtacgag ggcgaggacg
     2161 tgagcctgca gttcacccac gagagcatcc acaacgcccc cgacgaggcc ttcatggata
     2221 tcatcagact gcaccggccc gccatcacca gcagacgggg cctggtgcgc ttcagcagaa
     2281 tcggccagcg gggcagcatg tacacccgga gcggccagca catcggcgcc agaattcact
     2341 acttccagga catcagccct gtgacccagg ccgccgagga gatcgagctg caccccctgg
     2401 tggccgccga gaacgacacc ttcgacatct acgccgagcc cttcgaccct atccccgacc
     2461 ccgtgcagca cagcgtgacc cagagctacc tgaccagcac ccctaacacc ctgagccaga
     2521 gctggggcaa caccaccgtg cccctgagca tccccagcga ctggttcgtg cagagcggcc
     2581 ccgacatcac cttccctacc gctagcatgg gcaccccctt cagccccgtg acccctgccc
     2641 tgcccaccgg ccccgtgttc atcaccggca gcgacttcta cctgcaccct acctggtact
     2701 tcgcccggcg gagacggaag cggatccccc tgttcttcac cgacgtggcc gcctaagctt
     2761 atcgataccg tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     2821 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     2881 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     2941 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     3001 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     3061 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     3121 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     3181 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     3241 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     3301 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     3361 ctttgggccg cctccccgca tcgataccgt cggccgcttc cctttagtga gggttaatgc
     3421 ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     3481 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     3541 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     3601 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gaattcttaa
     3661 ttaactagcc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     3721 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     3781 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     3841 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     3901 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     3961 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     4021 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     4081 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     4141 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     4201 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     4261 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     4321 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     4381 ttttgctcac atgttcttaa ttaagctgta cactgtggaa tgtgtgtcag ttagggtgtg
     4441 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     4501 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4561 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     4621 ccagttccgc ccattctccg ccccatgact gactaatttt ttttatttat gcagaggccg
     4681 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     4741 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt
     4801 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgactcac tataggaggg
     4861 ccaccatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     4921 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     4981 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     5041 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     5101 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     5161 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     5221 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     5281 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     5341 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc
     5401 ccgacggcga ggatctcgtc gtgacacatg gcgatgcctg cttgccgaat atcatggtgg
     5461 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     5521 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     5581 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     5641 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgaattc
     5701 gctagaggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
     5761 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
     5821 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
     5881 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
     5941 gcagccaacg tcggggcggc aggccctgcc atag
//
