LOCUS       sequence-469485-       11310 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains mouse IgG2c CH1/CH2/CH3 domains, for cloning of antibody VH
            domains using SapI restriction enzyme. Full Fc-mediated immune 
            effector functions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11310)
  AUTHORS   Vazquez-Lombardi R, Nevoltris D, Rouet R, Christ D
  TITLE     Expression of IgG Monoclonals with Engineered Immune Effector 
            Functions.
  JOURNAL   Methods Mol Biol. 2018;1827:313-334. doi: 
            10.1007/978-1-4939-8648-4_16.
  PUBMED    30196504
REFERENCE   2  (bases 1 to 11310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2018"; volume: "1827:313-334. doi"; pages: " 
            10.1007/978-1-4939-8648-4_16"
COMMENT     Sequence Label: pRVL-2
FEATURES             Location/Qualifiers
     source          1..11310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      626..635
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1725..1859
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1749..1768)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1803..1822
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2002..2024)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2584..4373
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(7101..7119)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7187..7291
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7292..7360,7361..8152)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7510..7529)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8323..8911
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8812..8831
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9342..9487
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9530..10567
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(10565..10584)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10609..10657
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatctctag aagctgggta ccgccgccac catgggctgg agctgcatca tcctgttcct
      661 ggtggccacc gccaccggcg tgcacagcga agagctacag agtgagctct tcgccaagac
      721 caccgccccc agcgtgtacc ccctggcccc cgtgtgcggc ggcaccaccg gcagcagcgt
      781 gaccctgggc tgcctggtga agggctactt ccccgagccc gtgaccctga cctggaacag
      841 cggcagcctg agcagcggcg tgcacacctt ccccgccctg ctgcagagcg gcctgtacac
      901 cctgagcagc agcgtgaccg tgaccagcaa cacctggccc agccagacca tcacctgcaa
      961 cgtggcccac cccgccagca gcaccaaggt ggacaagaag atcgagccta gggtgcccat
     1021 cacccagaac ccctgccccc ctctgaagga gtgcccaccc tgcgccgccc ccgacctgct
     1081 gggcggcccc agcgtgttca tcttcccccc caagatcaag gacgtgctga tgatcagcct
     1141 gagccccatg gtgacctgcg tggtggtgga cgtgagcgag gacgaccccg acgtgcagat
     1201 cagctggttc gtgaacaacg tggaggtgca caccgcccag acccagaccc acagggagga
     1261 ctacaacagc accctgaggg tggtgagcgc cctgcccatc cagcaccagg actggatgag
     1321 cggcaaggag ttcaagtgca aggtgaacaa cagggccctg ccaagcccta tcgagaagac
     1381 catcagcaag cccaggggcc ccgtgagggc cccccaggtg tacgtgctgc ctcccccagc
     1441 cgaggagatg accaagaagg agttcagcct gacctgcatg atcaccggct tcctgcccgc
     1501 cgagatcgcc gtggactgga ccagcaacgg caggaccgag cagaactaca agaacaccgc
     1561 caccgtgctg gacagcgacg gcagctactt catgtacagc aagctgaggg tgcagaaaag
     1621 cacctgggag aggggcagcc tgttcgcctg cagcgtggtg cacgagggcc tgcacaacca
     1681 cctgaccacc aagaccatca gcaggagcct gggcaagtga taaggatcca gacatgataa
     1741 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     1801 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     1861 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     1921 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatccggct gcctcgcgcg
     1981 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     2041 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     2101 gtgtcggggc gcagccatga ggtcgactct agaggatcga tccccgcccc ggacgaacta
     2161 aacctgacta cgacatctct gccccttctt cgcggggcag tgcatgtaat cccttcagtt
     2221 ggttggtaca acttgccaac tgggccctgt tccacatgtg acacgggggg ggaccaaaca
     2281 caaaggggtt ctctgactgt agttgacatc cttataaatg gatgtgcaca tttgccaaca
     2341 ctgagtggct ttcatcctgg agcagacttt gcagtctgtg gactgcaaca caacattgcc
     2401 tttatgtgta actcttggct gaagctctta caccaatgct gggggacatg tacctcccag
     2461 gggcccagga agactacggg aggctacacc aacgtcaatc agaggggcct gtgtagctac
     2521 caataagcgg accctcaaga gggcattagc aatagtgttt ataaggcccc cttgttaacc
     2581 ctaaacgggt agcatatgct tcccgggtag tagtatatac tatccagact aaccctaatt
     2641 caatagcata tgttacccaa cgggaagcat atgctatcga attagggtta gtaaaagggt
     2701 cctaaggaac agcgatatct cccaccccat gagctgtcac ggttttattt acatggggtc
     2761 aggattccac gagggtagtg aaccatttta gtcacaaggg cagtggctga agatcaagga
     2821 gcgggcagtg aactctcctg aatcttcgcc tgcttcttca ttctccttcg tttagctaat
     2881 agaataactg ctgagttgtg aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc
     2941 aggtgacgcc cccagaataa aatttggacg gggggttcag tggtggcatt gtgctatgac
     3001 accaatataa ccctcacaaa ccccttgggc aataaatact agtgtaggaa tgaaacattc
     3061 tgaatatctt taacaataga aatccatggg gtggggacaa gccgtaaaga ctggatgtcc
     3121 atctcacacg aatttatggc tatgggcaac acataatcct agtgcaatat gatactgggg
     3181 ttattaagat gtgtcccagg cagggaccaa gacaggtgaa ccatgttgtt acactctatt
     3241 tgtaacaagg ggaaagagag tggacgccga cagcagcgga ctccactggt tgtctctaac
     3301 acccccgaaa attaaacggg gctccacgcc aatggggccc ataaacaaag acaagtggcc
     3361 actctttttt ttgaaattgt ggagtggggg cacgcgtcag cccccacacg ccgccctgcg
     3421 gttttggact gtaaaataag ggtgtaataa cttggctgat tgtaaccccg ctaaccactg
     3481 cggtcaaacc acttgcccac aaaaccacta atggcacccc ggggaatacc tgcataagta
     3541 ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg gaggacaaat tacacacact
     3601 tgcgcctgag cgccaagcac agggttgttg gtcctcatat tcacgaggtc gctgagagca
     3661 cggtgggcta atgttgccat gggtagcata tactacccaa atatctggat agcatatgct
     3721 atcctaatct atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     3781 atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt agcataggct
     3841 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     3901 atcctaatct gtatccgggt agcatatgct atcctaatag agattagggt agtatatgct
     3961 atcctaattt atatctgggt agcatatact acccaaatat ctggatagca tatgctatcc
     4021 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagca taggctatcc
     4081 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc
     4141 taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc
     4201 taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc
     4261 tcatgcatat acagtcagca tatgataccc agtagtagag tgggagtgct atcctttgca
     4321 tatgccgcca cctcccaagg gggcgtgaat tttcgctgct tgtccttttc ctgctggttg
     4381 ctcccattct taggtgaatt taaggaggcc aggctaaagc cgtcgcatgt ctgattgctc
     4441 accaggtaaa tgtcgctaat gttttccaac gcgagaaggt gttgagcgcg gagctgagtg
     4501 acgtgacaac atgggtatgc ccaattgccc catgttggga ggacgaaaat ggtgacaaga
     4561 cagatggcca gaaatacacc aacagcacgc atgatgtcta ctggggattt attctttagt
     4621 gcgggggaat acacggcttt taatacgatt gagggcgtct cctaacaagt tacatcactc
     4681 ctgcccttcc tcaccctcat ctccatcacc tccttcatct ccgtcatctc cgtcatcacc
     4741 ctccgcggca gccccttcca ccataggtgg aaaccaggga ggcaaatcta ctccatcgtc
     4801 aaagctgcac acagtcaccc tgatattgca ggtaggagcg ggctttgtca taacaaggtc
     4861 cttaatcgca tccttcaaaa cctcagcaaa tatatgagtt tgtaaaaaga ccatgaaata
     4921 acagacaatg gactccctta gcgggccagg ttgtgggccg ggtccagggg ccattccaaa
     4981 ggggagacga ctcaatggtg taagacgaca ttgtggaata gcaagggcag ttcctcgcct
     5041 taggttgtaa agggaggtct tactacctcc atatacgaac acaccggcga cccaagttcc
     5101 ttcgtcggta gtcctttcta cgtgactcct agccaggaga gctcttaaac cttctgcaat
     5161 gttctcaaat ttcgggttgg aacctccttg accacgatgc tttccaaacc accctccttt
     5221 tttgcgcctg cctccatcac cctgaccccg gggtccagtg cttgggcctt ctcctgggtc
     5281 atctgcgggg ccctgctcta tcgctcccgg gggcacgtca ggctcaccat ctgggccacc
     5341 ttcttggtgg tattcaaaat aatcggcttc ccctacaggg tggaaaaatg gccttctacc
     5401 tggagggggc ctgcgcggtg gagacccgga tgatgatgac tgactactgg gactcctggg
     5461 cctcttttct ccacgtccac gacctctccc cctggctctt tcacgacttc cccccctggc
     5521 tctttcacgt cctctacccc ggcggcctcc actacctcct cgaccccggc ctccactacc
     5581 tcctcgaccc cggcctccac tgcctcctcg accccggcct ccacctcctg ctcctgcccc
     5641 tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc
     5701 tgcccctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctcc
     5761 tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctgctcctgc
     5821 ccctcctgcc cctcctgctc ctgcccctcc tgcccctcct gctcctgccc ctcctgctcc
     5881 tgcccctcct gctcctgccc ctcctgctcc tgcccctcct gcccctcctg cccctcctcc
     5941 tgctcctgcc cctcctgctc ctgcccctcc tgcccctcct gcccctcctg ctcctgcccc
     6001 tcctcctgct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc
     6061 ccctcctcct gctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctgcccctcc
     6121 tgcccctcct gcccctcctc ctgctcctgc ccctcctcct gctcctgccc ctcctgcccc
     6181 tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgcccctc ctcctgctcc
     6241 tgcccctcct gcccctcctg cccctcctcc tgctcctgcc cctcctcctg ctcctgcccc
     6301 tcctgcccct cctgcccctc ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc
     6361 ccctcctgct cctgcccctc ccgctcctgc tcctgctcct gttccaccgt gggtcccttt
     6421 gcagccaatg caacttggac gtttttgggg tctccggaca ccatctctat gtcttggccc
     6481 tgatcctgag ccgcccgggg ctcctggtct tccgcctcct cgtcctcgtc ctcttccccg
     6541 tcctcgtcca tggttatcac cccctcttct ttgaggtcca ctgccgccgg agccttctgg
     6601 tccagatgtg tctcccttct ctcctaggcc atttccaggt cctgtacctg gcccctcgtc
     6661 agacatgatt cacactaaaa gagatcaata gacatcttta ttagacgacg ctcagtgaat
     6721 acagggagtg cagactcctg ccccctccaa cagccccccc accctcatcc ccttcatggt
     6781 cgctgtcaga cagatccagg tctgaaaatt ccccatcctc cgaaccatcc tcgtcctcat
     6841 caccaattac tcgcagcccg gaaaactccc gctgaacatc ctcaagattt gcgtcctgag
     6901 cctcaagcca ggcctcaaat tcctcgtccc cctttttgct ggacggtagg gatggggatt
     6961 ctcgggaccc ctcctcttcc tcttcaaggt caccagacag agatgctact ggggcaacgg
     7021 aagaaaagct gggtgcggcc tgtgaggatc agcttatcga tgataagctg tcaaacatga
     7081 gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     7141 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     7201 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     7261 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     7321 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     7381 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     7441 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     7501 taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
     7561 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     7621 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     7681 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     7741 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     7801 cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa
     7861 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     7921 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     7981 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     8041 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     8101 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     8161 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     8221 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     8281 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     8341 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     8401 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     8461 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     8521 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     8581 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     8641 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     8701 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     8761 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     8821 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     8881 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     8941 cctggccttt tgctggcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag
     9001 catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa
     9061 ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccccgag
     9121 atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg
     9181 gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat
     9241 ccgttagcga ggtgccgccc tgcttcatcc ccgtggcccg ttgctcgcgt ttgctggcgg
     9301 tgtccccgga agaaatatat ttgcatgtct ttagttctat gatgacacaa accccgccca
     9361 gcgtcttgtc attggcgaat tcgaacacgc agatgcagtc ggggcggcgc ggtccgaggt
     9421 ccacttcgca tattaaggtg acgcgtgtgg cctcgaacac cgagcgaccc tgcagcgacc
     9481 cgcttaacag cgtcaacagc gtgccgcaga tcccgggggg caatgagata tgaaaaagcc
     9541 tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga
     9601 cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg
     9661 tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta
     9721 tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag
     9781 cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc
     9841 tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc
     9901 ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata
     9961 cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac
    10021 tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg
    10081 ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt
    10141 cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga
    10201 ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca
    10261 gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc
    10321 gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga
    10381 tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt
    10441 cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt
    10501 actcgccgat agtggaaacc gacgccccag cactcgtccg gatcgggaga tgggggaggc
    10561 taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa
    10621 gacagaataa aacgcacggg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
    10681 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
    10741 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
    10801 ggggcggcag gccctgccat agccactggc cccgtgggtt agggacgggg tcccccatgg
    10861 ggaatggttt atggttcgtg ggggttatta ttttgggcgt tgcgtggggt caggtccacg
    10921 actggactga gcagacagac ccatggtttt tggatggcct gggcatggac cgcatgtact
    10981 ggcgcgacac gaacaccggg cgtctgtggc tgccaaacac ccccgacccc caaaaaccac
    11041 cgcgcggatt tctggcgtgc caagctagtc gaccaattct catgtttgac agcttatcat
    11101 cgcagatccg ggcaacgttg ttgccattgc tgcaggcgca gaactggtag gtatggaaga
    11161 tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa
    11221 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
    11281 ttggctcatg tccaatatga ccgccatgtt
//
