LOCUS       sequence-46949-e       10126 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10126)
  AUTHORS   Kon T, Oyama T, Shimo-Kon R, Imamula K, Shima T, Sutoh K, Kurisu G
  TITLE     The 2.8 A crystal structure of the dynein motor domain.
  JOURNAL   Nature. 2012 Mar 7;484(7394):345-50. doi: 10.1038/nature10955.
  PUBMED    22398446
REFERENCE   2  (bases 1 to 10126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-03-7- 7"; volume: "484"; issue: "7394"; pages:
            "345-50"
COMMENT     Sequence Label: pMB38-HF380
FEATURES             Location/Qualifiers
     source          1..10126
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..37
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             47..70
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agatctaaaa atgacgcgtc atcatcatca tcatcatggt ggtggtgatt ataaagatga
       61 tgatgataaa ggtggtggta aggtaccagt cgaagaggag attcaagatt tgaaagcagt
      121 ttgggtcgaa ctttcaaata catggcaaga aatcgatagt cttaaagaga ctgcttggtc
      181 tgctatcatt ccaagaaaag ttagaaaatc attggaagat actttacaaa aattgaaaaa
      241 tttaccaaat cgtattcgtc aatactctgc ctttgatcat gctcaaaact taattaaaat
      301 ctatctcaaa ggtaatgcaa tcatcaccga tcttcactct gaagccatca aagatcgtca
      361 ttggaaaatt ttaaagaaac gtttaaatac caattggatc attacagagt taacattggg
      421 ttccatttgg gattcagatt tagcaagaaa tgaaaatatc tatcgtgagg ttatcactgc
      481 agctcaaggt gaaattgctt tagaggaatt ccttaaaggt gttagagaat tttggacaac
      541 tctcgaattg gatctcgtta actatcaacg taaatgtaaa ttggtacgtg gttgggatga
      601 tttattcaac aaattggcag aacatttgaa ttcaatttcc gctatgaaaa tgtcaccata
      661 ctataaggtt ttcgaagagg aagcaaatca ttgggatgat agattaaata aggttagatc
      721 attattggat gtttggatcg atgttcaaag acgttgggtc tatttagaag gtattttcag
      781 tggtagtggt gatatcaatc aattgttacc agctgaatca actcgtttca agagtatcaa
      841 ctctgaattc attgcaatcc ttaaaaaggt tagtggtgca ccattgattt tggaagtatt
      901 ggccattgaa agaattcaac aaactatgga acgtttatcc gatctcttgg gtaaagttca
      961 aaaagcattg ggtgaatatt tggagagaca aagatctgca tttgctcgtt tctatttcgt
     1021 aggtgatgaa gatcttttgg aaatcattgg taatagtaaa gatatcatta agattcaaaa
     1081 gcatttcaga aagatgtttg ctggtttggc taatttaact ttggatgatg aaaaaaccac
     1141 cattattggt atgtcctctg ctgaaggtga aaccgttacc tttaagaaac caatttccat
     1201 cgctaatggt ccaaagattc atgaatggtt aacaatggtt gaatctgaaa tgaaatcaac
     1261 tttggcaact ttattaagtg aatcattaca acatttcaat caagtcgatg ttaatgatca
     1321 ttctaaatac tctgaatggg ttgataatta tccaactcaa ttggtccttc ttacaagtca
     1381 aattgtttgg tccactcaag tcgatcaagc tttaggtggt ggtacattac aacaatcaaa
     1441 gattcaagaa caattacaat ccattgaaca aaccactcaa atgattttaa ataatttggc
     1501 cgatagtgtt ttacaagatt tatccgctca aaagagaaag aaattcgaac atttaattac
     1561 agagttggtt catcaacgtg atgtggtcag acaattacaa aaatgtaaaa accttactgg
     1621 taataaagat tttgattggc tctatcatat gcgttactat tatgatgcta ctcaagagaa
     1681 tgttcttcat aaattggtca tccatatggc aaatgctact ttttattatg gtttcgaata
     1741 tttaggtatt ggtgaacgtt tggttcaaac tccattaact gatcgttgtt atcttacact
     1801 aactcaagca ttggagagta gaatgggtgg taatccattt ggtccagcag gtactggtaa
     1861 aactgaaacc gttaaagcat tgggtagtca attgggtcgt ttcgtattgg ttttctgttg
     1921 tgatgaaggt tttgatcttc aagctatgtc tcgtattttc gttggtcttt gtcaatgtgg
     1981 tgcttggggt tgtttcgatg agttcaatcg tttggaggaa agaattctct ctgctgtctc
     2041 tcaacaaatt caaaccattc aagtcgctct caaagagaat tccaaagagg ttgaactttt
     2101 aggtggtaaa aatatttcac tccaccaaga tatgggtatt tttgtaacaa tgaatccagg
     2161 ttatgcaggt cgttccaatt taccagataa tttaaagaaa cttttccgtt caatggccat
     2221 gattaaacca gatcgtgaaa tgattgctca agttatgtta tacagtcaag gctttaaaac
     2281 tgcagaagtt ttagccggta aaatcgttcc actctttaaa ctttgtcaag aacaattgtc
     2341 agctcaatct cattatgatt ttggtttacg tgctctcaag agtgttttag ttagtgcagg
     2401 tggtatcaag agaaaatgtc aaccaccaca attaccacca atcactgatg ctgaatcaaa
     2461 aactaaagct gatcaaatct attgtcaata tgaaattggt gtgttattaa actctatcaa
     2521 tgataccatg attccaaaat tggttgctga cgatattcca ttgattcaat cattgttatt
     2581 ggatgttttc ccaggttcac aattacaacc aattcaaatg gatcaattac gtaaaaagat
     2641 tcaagaaatt gccaaacaaa gacatttggt taccaaacaa gaatgggttg aaaagatcct
     2701 tcaattacat caaattctca atatcaatca tggtgttatg atggttggtc catctggtgg
     2761 tggtaaaact acaagttggg aggtttattt agaagccatc gaacaagttg ataacattaa
     2821 atccgaagct catgttatgg atccaaaagc tatcaccaaa gatcaattat ttggttcatt
     2881 ggatttaaca actcgtgaat ggaccgatgg tttattcact gcaacccttc gtcgtatcat
     2941 tgataatgtt cgtggtgaat ccactaaacg tcattggatc attttcgatg gtgatgttga
     3001 tccagaatgg gtagaaaatt tgaactctct cttggatgat aataaattat taacacttcc
     3061 aaatggtgaa cgtttagcac ttccaaataa tgttagagtt atgtttgaag tacaagatct
     3121 taaatatgca acactcgcta ccatttcacg ttgtggtatg gtttggttta gtgaagagat
     3181 tttaacaact caaatgattt tccaaaatta tttagatact ctttcaaatg aaccatttga
     3241 tccacaagaa aaagaacaac aaaaaagaaa tgaaaatgct caattacaac aacaacaaca
     3301 aacaactatt acttcaccaa ttttaacttc accaccaact acatcatcat catcacgttc
     3361 aaccacttca acaacttcaa tgataccagc tggattaaaa gttcaaaaag aatgtgcagc
     3421 aattatttca caatattttg aaccaggtgg tttggttcat aaagttttag aagatgctgg
     3481 tcaaagacca catattatgg atttcactcg tcttagagta ttaaattctt tcttctcctt
     3541 aatgaataga tcaattgtca atgttattga atataatcaa ttacattcag atttcccaat
     3601 gtcaccagaa aatcaaagta attatattac caatcgttta ctttattcat taatgtgggg
     3661 tttaggtggt agtatgggtt tagttgaaag agagaacttc tctaaattca ttcaaaccat
     3721 tgcaattact ccagttccag ccaatactat tccattgtta gattattccg tatcaattga
     3781 tgatgccaat tggagtttat ggaagaataa agtaccatca gttgaagttg aaactcataa
     3841 agttgcctca ccagatgttg tcattccaac cgttgataca actcgtcatg ttgatgtatt
     3901 acatgcttgg ttatctgaac atcgtccatt gattctttgt ggtccaccag gttctggtaa
     3961 aaccatgaca ctcacaagta cacttcgtgc tttcccagac tttgaggtgg tttcattgaa
     4021 tttcagttca gcaacaacac cagaactttt actcaagaca ttcgatcatc attgtgaata
     4081 taaacgtaca ccaagtggtg aaactgtact tcgtccaact caattaggta aatggttagt
     4141 agtattttgt gatgaaatca atcttccatc cactgataaa tatggcactc aacgtgttat
     4201 tacattcatt cgtcaaatgg ttgaaaaagg tggtttctgg agaacatccg atcatacttg
     4261 gattaaattg gataagattc aattcgttgg tgcatgtaat ccaccaactg atgctggtcg
     4321 tgttcaatta actcatcgtt tccttagaca tgctccaatc ttattggtag atttcccatc
     4381 aacttcatct ctcactcaaa tctatggtac ttttaataga gccttaatga aacttttacc
     4441 aaatcttcgt tcattcgctg ataatttaac cgatgcaatg gttgaattct atagtgaatc
     4501 tcaaaaacgt ttcactcctg atattcaagc tcattacatt tattcaccac gtgaactctc
     4561 tcgttgggat cgtgctctct tagaggcaat tcaaactatg gatggttgta cattggaagg
     4621 tttagtacgt ttatgggccc atgaagctct tagattattc caagatcgtc tcgttgaaac
     4681 tgaagaaaaa gaatggaccg ataaaaagat tgatgaagta gctcttaaac atttcccatc
     4741 tgttaattta gatgctttga aacgtccaat cctctatagt aattggttaa ccaaagatta
     4801 tcaaccagtt aatcgttcag atcttcgtga atatgtcaaa gcaagattaa aggtattcta
     4861 tgaagaagag ttggatgttc cattggtatt attcaatgaa gtattggatc atattcttag
     4921 aattgatcgt gttttccgtc aaccacaagg tcatgcactt ttaattggtg tatctggtgg
     4981 tggtaaatca gttttatcac gttttgtcgc ttggatgaat ggtttatcaa tctatacaat
     5041 caaagttaac aacaattata aatcctctga tttcgatgat gatcttcgta tgttattaaa
     5101 acgtgcaggt tgtaaagaag agaagatttg tttcattttc gatgaatcca atgttttaga
     5161 atcatctttc cttgaacgta tgaatactct attggcaggt ggtgaagttc caggtctttt
     5221 cgaaggtgaa gaattcactg cactcatgca tgcttgtaaa gaaaccgccc aaagaaatgg
     5281 tctcatttta gattctgaag aagaacttta caaatacttt acaagtcaag ttcgtcgtaa
     5341 tcttcatgta gttttcacaa tgaatccagc aagtccagat ttccacaatc gttcagctac
     5401 ctctcctgca cttttcaatc gttgtgtttt ggattggttt ggtgaatggt caccagaagc
     5461 tttattccaa gttggttctg aattcactag aaatctcgat cttgaaaatc cacaatacat
     5521 tgctccacca gttttcattc aagaagcaga gataatgggt aataatttaa tggccatccc
     5581 accatcacat cgtgatgcag tcgtaagttc attggtttat attcatcaaa ccattggtga
     5641 agccaatatt cgtttactta aacgtcaagg tcgtcaaaat tatgtaactc cacgtcatta
     5701 tttggatttc attaatcaag tggtattatt aatcaatgag aaacgtgatc aattagaaga
     5761 ggaacaactt catctcaata ttggtcttaa gaaattacgt gatactgagg ctcaagttaa
     5821 agatcttcaa gttagtttag ctcaaaagaa tcgtgaactc gatgttaaga atgaacaagc
     5881 caatcaaaaa ttaaaacaaa tggttcaaga tcaacaagca gctgaaatta aacaaaagga
     5941 tgctcgtgaa cttcaagttc aattggatgt tagaaacaaa gagattgccg ttcaaaaagt
     6001 taaagcttat gcagatttag aaaaagctga accagccatt atcgaagctc aagaagctgt
     6061 atccaccatt aaaaagaaac atttggatga aattaaatct ttaccaaaac caccaactcc
     6121 agtcaaattg gcaatggaag cagtttgttt aatgttgggt ggtaagaaat tagaatgggc
     6181 agatattaga aagaagatta tggaaccaaa tttcattacc tcgattatta attatgatac
     6241 aaagaaaatg atgacaccaa aaatcagaga agccattacc aaaggttatt tagaagatcc
     6301 aggtttcgat tatgaaacag ttaatcgtgc ctccaaagct tgtggtccat tggttaaatg
     6361 ggcaaccgct caaacttatt actctgaaat tttagatcgt attaaaccat tacgtgaaga
     6421 ggttgaacaa ttagaaaacg ctgcaaatga attgaaattg aaacaagatg aaatcgttgc
     6481 caccattact gccttggaga aatctattgc cacctataag gaagagtatg caactttaat
     6541 tcgtgaaact gaacaaatta aaaccgaatc aagtaaagtc aagaataagg tcgatcgttc
     6601 aattgctctt ttagataatc ttaactctga acgtggtaga tgggaacaac aaagtgaaaa
     6661 ctttaacact caaatgtcaa ctgttgttgg tgatgttgtt ttagcatctg ctttccttgc
     6721 atatattggt ttctttgatc aaaacttccg tactgacctt atgagaaaat ggatgattcg
     6781 tttagattca gttggtatta aattcaagag tgatctatca gtaccatctt tcctttccaa
     6841 accagaagaa cgtcttaact ggcatgcaaa ttctttacca tccgatgaat tatgtattga
     6901 aaatgcaatc atgcttaaac gtttcaatcg ttatccattg gttatagatc catctggtca
     6961 agccatggaa ttccttatga atcaatatgc cgacaaaaag attaccaaaa ctagtttcct
     7021 cgattcatca tttatgaaga atttagagag tgctctcaga tttggttgtc cattattggt
     7081 acaagatgtt gaaaacattg atccagtatt gaatccagta ctcaataaag aaattcgtaa
     7141 gaaaggtggt cgtattctca ttcgtttagg tgatcaagat gtcgatttct ctccatcctt
     7201 tatgatcttt ttgttcactc gtgacccaac cgctcacttt actccagatc tttgttctcg
     7261 tgttaccttt gtaaatttca ctgtcactcc atcctcactt caaagtcaat gtcttcatga
     7321 agctcttaaa actgaaagac cagatactca taagaaacgt tctgatctcc tcaagattca
     7381 aggtgaattc caagttaaat taagaattct cgaaaaatct ttattgaatg ctctttccca
     7441 agcctctggt aacattttag atgatgattc tgtcatttca acccttgaaa cattaaagaa
     7501 agaaaccact gaaattgcac ttaaagttga agaaaccgag accgttatgc aagaaattag
     7561 tgaagtttca gctctttata atccaatggc tctctcatgt tcacgtgttt actttgcaat
     7621 ggaagaactt tctcaattcc atctctacca attctcttta cgtgctttct tggatatctt
     7681 ttataatcta cttaataata atccaaattt agtcgataaa aaagatccaa atgaacgttt
     7741 ggtttattta tcaaaagata ttttctcaat gactttcaat cgtgtaactc gtacactctt
     7801 aaatgatgat aaattaacat ttgcacttca attaactatc atttcagtta aaggtacatc
     7861 caatgaaatc gaagaaagtg aatgggactt ccttttgaaa ggtggtgata atttaacatc
     7921 aatcaaagaa accattccac aattggattc attattatca actactcaac aaaaatggtt
     7981 aatttgtctt agacaacaag ttccatcatt ctctaaattg gttgatcata ttcaacaaaa
     8041 ctcttccgat tggaaacaat tctttggtaa agatcaagtt ggtgaaccaa tcattccaga
     8101 atcttggatc gtagctcaag ctcaactttc taatcaacaa tcaaccattg tttcaaactt
     8161 tagaaaaatt cttttaatga aagctttcca tagtgataga gttttacaat attctcatag
     8221 ttttgtttgt tcagtatttg gtgaagattt cttaaatact caagaattag atatggcaaa
     8281 cattgtagag aaagaggtta aatcttcatc accattgtta ttatgttcag ttccaggtta
     8341 tgatgcatca agtaaagtcg atgatttagc attacaatta cataaacaat ataagtcatt
     8401 tgctattggt tcacctgaag gttttgaatt agccgagaaa tcaatttatg ccgccgctaa
     8461 atctggtact tgggtattat taaagaatat tcatttggca ccacaatggt tggtacaatt
     8521 ggaaaagaaa cttcattcac tctctccaca tccaagcttt agattattta tgacctctga
     8581 aattcatcca gctcttccag caaatcttct tcgtatgtca aatgtattct cttatgaaaa
     8641 tccaccaggt gttaaagcta atcttttaca tacctttatt ggtattcctg caactcgtat
     8701 ggataaacaa ccagcagaac gttcacgtat ctatttcctt ttagcttggt tccatgcaat
     8761 cattcaagaa agacttcgtt atattccatt gggttggact aaattctttg aattcaatga
     8821 tgccgattta cgtggtgccc tagattcaat cgattattgg gttgatctct atagtaaagg
     8881 tagaagtaac attgatccag acaagattcc atggatcgct gttcgtacca ttttaggtag
     8941 tacaatttat ggtggtagaa ttgataatga atttgatatg cgtttacttt attcattcct
     9001 tgaacaactt tttactccaa gtgcattcaa tccagatttc ccattagtac catccattgg
     9061 tttatcagtt ccagaaggta caactcgtgc tcactttatg aaatggattg aagctttacc
     9121 agagatttca actccaattt ggttaggttt acctgaaaat gctgaatcat tactcttgtc
     9181 aaataaagct agaaagatga ttaatgatct ccaaaaaatg caatcatctg aagaagatgg
     9241 tgaagatgat caagttagtg gtagttcaaa gaaagaatca tcatcatcat catcagaaga
     9301 taaaggtaaa gctaaactta gagcaaccat cactgaatgg actaaactct taccaaaacc
     9361 attgaaacaa ttaaagagaa ccactcaaaa tattaaagat ccattattcc gttgctttga
     9421 aagagaaatt tcaactggtg gtaaattagt taaaaagatt accaatgatc tcgcaaatct
     9481 tttagaacta atttctggta atattaaatc aaccaattat cttcgttcac ttaccacttc
     9541 aatctctaaa ggtatcgttc caaaagaatg gaaatggtat tcagtacctg aaaccattag
     9601 tctttcagtt tggatctctg atttctcaaa gagaatgcaa caattatcag agatttcaga
     9661 aagttcagat tatagttcca ttcaagtttg gttgggtggt ttattaaatc cagaagctta
     9721 cattaccgct actagacaat ccgcttctca attgaatggt tggtccttag aaaaccttag
     9781 attacacgct tcctcattgg gtaaaatctc ttcagaaggt ggtgctagtt tcaatgttaa
     9841 aggtatggct ttagaaggtg ctgtttggaa caatgatcaa ctcacaccaa ccgatatcct
     9901 ctcaactcca atttcaatcg ctactttaac ttggaaagat aaagatgatc caatctttaa
     9961 caattcatct tcaaaactat ctgtaccagt ctatctcaat gaaactagat cggaattatt
    10021 attctctata gatttaccat atgatcaatc aacttcaaaa caaaattggt atcaaagatc
    10081 agtttcaatc agttcatgga aatctgatat ttaagagctc acgcgt
//
