LOCUS       sequence-469496-        5295 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5295)
  TITLE     TLR Signaling Plasmids
REFERENCE   2  (bases 1 to 5295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGL3-ELAM-luc
FEATURES             Location/Qualifiers
     source          1..5295
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    8..56
                     /note="synthetic polyadenylation signal"
     misc_feature    70..161
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     110..129
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      481..490
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             487..2139
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(553..571)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2180..2301
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2191..2210)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2245..2264
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2623..2643
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(2795..2812)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2966..3554)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3046..3065)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3725..4516,4517..4585))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4348..4367
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4586..4690)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4717..5172
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4804..4823)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5014..5035
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc
       61 gatagtacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata
      121 ggctgtcccc agtgcaagtg caggtgccag aacatttctc tatcgatagg taccgagctc
      181 ttacgcgtgc tagaatcatg catttttgtc atattaataa aattgcatat acgatataaa
      241 ggcatggaca aaggtgaagt agcttcaaga gacagagttt ctgacatcat tgtaatttta
      301 agcatcgtgg atattcccgg gaaagttttt ggatgccatt ggggatttcc tctttactgg
      361 atgtggacaa tatcctccta taagcttggg gattacatag ttcaggactt gggcataaaa
      421 ggcagagcag ggcacggatc tggatctaag taagcttggc attccggtac tgttggtaaa
      481 gccaccatgg aagacgccaa aaacataaag aaaggcccgg cgccattcta tccgctggaa
      541 gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct ggttcctgga
      601 acaattgctt ttacagatgc acatatcgag gtggacatca cttacgctga gtacttcgaa
      661 atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa tcacagaatc
      721 gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc gttatttatc
      781 ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct caacagtatg
      841 ggcatttcgc agcctaccgt ggtgttcgtt tccaaaaagg ggttgcaaaa aattttgaac
      901 gtgcaaaaaa agctcccaat catccaaaaa attattatca tggattctaa aacggattac
      961 cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg ttttaatgaa
     1021 tacgattttg tgccagagtc cttcgatagg gacaagacaa ttgcactgat catgaactcc
     1081 tctggatcta ctggtctgcc taaaggtgtc gctctgcctc atagaactgc ctgcgtgaga
     1141 ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac tgcgatttta
     1201 agtgttgttc cattccatca cggttttgga atgtttacta cactcggata tttgatatgt
     1261 ggatttcgag tcgtcttaat gtatagattt gaagaagagc tgtttctgag gagccttcag
     1321 gattacaaga ttcaaagtgc gctgctggtg ccaaccctat tctccttctt cgccaaaagc
     1381 actctgattg acaaatacga tttatctaat ttacacgaaa ttgcttctgg tggcgctccc
     1441 ctctctaagg aagtcgggga agcggttgcc aagaggttcc atctgccagg tatcaggcaa
     1501 ggatatgggc tcactgagac tacatcagct attctgatta cacccgaggg ggatgataaa
     1561 ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga aggttgtgga tctggatacc
     1621 gggaaaacgc tgggcgttaa tcaaagaggc gaactgtgtg tgagaggtcc tatgattatg
     1681 tccggttatg taaacaatcc ggaagcgacc aacgccttga ttgacaagga tggatggcta
     1741 cattctggag acatagctta ctgggacgaa gacgaacact tcttcatcgt tgaccgcctg
     1801 aagtctctga ttaagtacaa aggctatcag gtggctcccg ctgaattgga atccatcttg
     1861 ctccaacacc ccaacatctt cgacgcaggt gtcgcaggtc ttcccgacga tgacgccggt
     1921 gaacttcccg ccgccgttgt tgttttggag cacggaaaga cgatgacgga aaaagagatc
     1981 gtggattacg tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt
     2041 gtggacgaag taccgaaagg tcttaccgga aaactcgacg caagaaaaat cagagagatc
     2101 ctcataaagg ccaagaaggg cggaaagatc gccgtgtaat tctagagtcg gggcggccgg
     2161 ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     2221 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     2281 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     2341 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat
     2401 aaggatctga acgatggagc ggagaatggg cggaactggg cggagttagg ggcgggatgg
     2461 gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc
     2521 tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc
     2581 tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat
     2641 tccacagcgg atccgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg
     2701 gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact
     2761 cgtaggacag gtgccggcag cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     2821 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     2881 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     2941 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     3001 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     3061 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     3121 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     3181 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3241 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3301 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3361 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     3421 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3481 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3541 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     3601 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     3661 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     3721 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     3781 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     3841 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     3901 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     3961 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     4021 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     4081 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     4141 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     4201 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     4261 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     4321 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     4381 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     4441 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     4501 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     4561 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     4621 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     4681 gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa
     4741 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     4801 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4861 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     4921 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     4981 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     5041 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     5101 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     5161 cgcttacaat ttgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     5221 gggcctcttc gctattacgc cagcccaagc taccatgata agtaagtaat attaaggtac
     5281 gggaggtact tggag
//
