LOCUS       sequence-46950-e        9760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9760)
  AUTHORS   Kon T, Oyama T, Shimo-Kon R, Imamula K, Shima T, Sutoh K, Kurisu G
  TITLE     The 2.8 A crystal structure of the dynein motor domain.
  JOURNAL   Nature. 2012 Mar 7;484(7394):345-50. doi: 10.1038/nature10955.
  PUBMED    22398446
REFERENCE   2  (bases 1 to 9760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-03-7- 7"; volume: "484"; issue: "7394"; pages:
            "345-50"
COMMENT     Sequence Label: pMB38-HF380deltaMTBD
FEATURES             Location/Qualifiers
     source          1..9760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..37
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             47..70
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agatctaaaa atgacgcgtc atcatcatca tcatcatggt ggtggtgatt ataaagatga
       61 tgatgataaa ggtggtggta aggtaccagt cgaagaggag attcaagatt tgaaagcagt
      121 ttgggtcgaa ctttcaaata catggcaaga aatcgatagt cttaaagaga ctgcttggtc
      181 tgctatcatt ccaagaaaag ttagaaaatc attggaagat actttacaaa aattgaaaaa
      241 tttaccaaat cgtattcgtc aatactctgc ctttgatcat gctcaaaact taattaaaat
      301 ctatctcaaa ggtaatgcaa tcatcaccga tcttcactct gaagccatca aagatcgtca
      361 ttggaaaatt ttaaagaaac gtttaaatac caattggatc attacagagt taacattggg
      421 ttccatttgg gattcagatt tagcaagaaa tgaaaatatc tatcgtgagg ttatcactgc
      481 agctcaaggt gaaattgctt tagaggaatt ccttaaaggt gttagagaat tttggacaac
      541 tctcgaattg gatctcgtta actatcaacg taaatgtaaa ttggtacgtg gttgggatga
      601 tttattcaac aaattggcag aacatttgaa ttcaatttcc gctatgaaaa tgtcaccata
      661 ctataaggtt ttcgaagagg aagcaaatca ttgggatgat agattaaata aggttagatc
      721 attattggat gtttggatcg atgttcaaag acgttgggtc tatttagaag gtattttcag
      781 tggtagtggt gatatcaatc aattgttacc agctgaatca actcgtttca agagtatcaa
      841 ctctgaattc attgcaatcc ttaaaaaggt tagtggtgca ccattgattt tggaagtatt
      901 ggccattgaa agaattcaac aaactatgga acgtttatcc gatctcttgg gtaaagttca
      961 aaaagcattg ggtgaatatt tggagagaca aagatctgca tttgctcgtt tctatttcgt
     1021 aggtgatgaa gatcttttgg aaatcattgg taatagtaaa gatatcatta agattcaaaa
     1081 gcatttcaga aagatgtttg ctggtttggc taatttaact ttggatgatg aaaaaaccac
     1141 cattattggt atgtcctctg ctgaaggtga aaccgttacc tttaagaaac caatttccat
     1201 cgctaatggt ccaaagattc atgaatggtt aacaatggtt gaatctgaaa tgaaatcaac
     1261 tttggcaact ttattaagtg aatcattaca acatttcaat caagtcgatg ttaatgatca
     1321 ttctaaatac tctgaatggg ttgataatta tccaactcaa ttggtccttc ttacaagtca
     1381 aattgtttgg tccactcaag tcgatcaagc tttaggtggt ggtacattac aacaatcaaa
     1441 gattcaagaa caattacaat ccattgaaca aaccactcaa atgattttaa ataatttggc
     1501 cgatagtgtt ttacaagatt tatccgctca aaagagaaag aaattcgaac atttaattac
     1561 agagttggtt catcaacgtg atgtggtcag acaattacaa aaatgtaaaa accttactgg
     1621 taataaagat tttgattggc tctatcatat gcgttactat tatgatgcta ctcaagagaa
     1681 tgttcttcat aaattggtca tccatatggc aaatgctact ttttattatg gtttcgaata
     1741 tttaggtatt ggtgaacgtt tggttcaaac tccattaact gatcgttgtt atcttacact
     1801 aactcaagca ttggagagta gaatgggtgg taatccattt ggtccagcag gtactggtaa
     1861 aactgaaacc gttaaagcat tgggtagtca attgggtcgt ttcgtattgg ttttctgttg
     1921 tgatgaaggt tttgatcttc aagctatgtc tcgtattttc gttggtcttt gtcaatgtgg
     1981 tgcttggggt tgtttcgatg agttcaatcg tttggaggaa agaattctct ctgctgtctc
     2041 tcaacaaatt caaaccattc aagtcgctct caaagagaat tccaaagagg ttgaactttt
     2101 aggtggtaaa aatatttcac tccaccaaga tatgggtatt tttgtaacaa tgaatccagg
     2161 ttatgcaggt cgttccaatt taccagataa tttaaagaaa cttttccgtt caatggccat
     2221 gattaaacca gatcgtgaaa tgattgctca agttatgtta tacagtcaag gctttaaaac
     2281 tgcagaagtt ttagccggta aaatcgttcc actctttaaa ctttgtcaag aacaattgtc
     2341 agctcaatct cattatgatt ttggtttacg tgctctcaag agtgttttag ttagtgcagg
     2401 tggtatcaag agaaaatgtc aaccaccaca attaccacca atcactgatg ctgaatcaaa
     2461 aactaaagct gatcaaatct attgtcaata tgaaattggt gtgttattaa actctatcaa
     2521 tgataccatg attccaaaat tggttgctga cgatattcca ttgattcaat cattgttatt
     2581 ggatgttttc ccaggttcac aattacaacc aattcaaatg gatcaattac gtaaaaagat
     2641 tcaagaaatt gccaaacaaa gacatttggt taccaaacaa gaatgggttg aaaagatcct
     2701 tcaattacat caaattctca atatcaatca tggtgttatg atggttggtc catctggtgg
     2761 tggtaaaact acaagttggg aggtttattt agaagccatc gaacaagttg ataacattaa
     2821 atccgaagct catgttatgg atccaaaagc tatcaccaaa gatcaattat ttggttcatt
     2881 ggatttaaca actcgtgaat ggaccgatgg tttattcact gcaacccttc gtcgtatcat
     2941 tgataatgtt cgtggtgaat ccactaaacg tcattggatc attttcgatg gtgatgttga
     3001 tccagaatgg gtagaaaatt tgaactctct cttggatgat aataaattat taacacttcc
     3061 aaatggtgaa cgtttagcac ttccaaataa tgttagagtt atgtttgaag tacaagatct
     3121 taaatatgca acactcgcta ccatttcacg ttgtggtatg gtttggttta gtgaagagat
     3181 tttaacaact caaatgattt tccaaaatta tttagatact ctttcaaatg aaccatttga
     3241 tccacaagaa aaagaacaac aaaaaagaaa tgaaaatgct caattacaac aacaacaaca
     3301 aacaactatt acttcaccaa ttttaacttc accaccaact acatcatcat catcacgttc
     3361 aaccacttca acaacttcaa tgataccagc tggattaaaa gttcaaaaag aatgtgcagc
     3421 aattatttca caatattttg aaccaggtgg tttggttcat aaagttttag aagatgctgg
     3481 tcaaagacca catattatgg atttcactcg tcttagagta ttaaattctt tcttctcctt
     3541 aatgaataga tcaattgtca atgttattga atataatcaa ttacattcag atttcccaat
     3601 gtcaccagaa aatcaaagta attatattac caatcgttta ctttattcat taatgtgggg
     3661 tttaggtggt agtatgggtt tagttgaaag agagaacttc tctaaattca ttcaaaccat
     3721 tgcaattact ccagttccag ccaatactat tccattgtta gattattccg tatcaattga
     3781 tgatgccaat tggagtttat ggaagaataa agtaccatca gttgaagttg aaactcataa
     3841 agttgcctca ccagatgttg tcattccaac cgttgataca actcgtcatg ttgatgtatt
     3901 acatgcttgg ttatctgaac atcgtccatt gattctttgt ggtccaccag gttctggtaa
     3961 aaccatgaca ctcacaagta cacttcgtgc tttcccagac tttgaggtgg tttcattgaa
     4021 tttcagttca gcaacaacac cagaactttt actcaagaca ttcgatcatc attgtgaata
     4081 taaacgtaca ccaagtggtg aaactgtact tcgtccaact caattaggta aatggttagt
     4141 agtattttgt gatgaaatca atcttccatc cactgataaa tatggcactc aacgtgttat
     4201 tacattcatt cgtcaaatgg ttgaaaaagg tggtttctgg agaacatccg atcatacttg
     4261 gattaaattg gataagattc aattcgttgg tgcatgtaat ccaccaactg atgctggtcg
     4321 tgttcaatta actcatcgtt tccttagaca tgctccaatc ttattggtag atttcccatc
     4381 aacttcatct ctcactcaaa tctatggtac ttttaataga gccttaatga aacttttacc
     4441 aaatcttcgt tcattcgctg ataatttaac cgatgcaatg gttgaattct atagtgaatc
     4501 tcaaaaacgt ttcactcctg atattcaagc tcattacatt tattcaccac gtgaactctc
     4561 tcgttgggat cgtgctctct tagaggcaat tcaaactatg gatggttgta cattggaagg
     4621 tttagtacgt ttatgggccc atgaagctct tagattattc caagatcgtc tcgttgaaac
     4681 tgaagaaaaa gaatggaccg ataaaaagat tgatgaagta gctcttaaac atttcccatc
     4741 tgttaattta gatgctttga aacgtccaat cctctatagt aattggttaa ccaaagatta
     4801 tcaaccagtt aatcgttcag atcttcgtga atatgtcaaa gcaagattaa aggtattcta
     4861 tgaagaagag ttggatgttc cattggtatt attcaatgaa gtattggatc atattcttag
     4921 aattgatcgt gttttccgtc aaccacaagg tcatgcactt ttaattggtg tatctggtgg
     4981 tggtaaatca gttttatcac gttttgtcgc ttggatgaat ggtttatcaa tctatacaat
     5041 caaagttaac aacaattata aatcctctga tttcgatgat gatcttcgta tgttattaaa
     5101 acgtgcaggt tgtaaagaag agaagatttg tttcattttc gatgaatcca atgttttaga
     5161 atcatctttc cttgaacgta tgaatactct attggcaggt ggtgaagttc caggtctttt
     5221 cgaaggtgaa gaattcactg cactcatgca tgcttgtaaa gaaaccgccc aaagaaatgg
     5281 tctcatttta gattctgaag aagaacttta caaatacttt acaagtcaag ttcgtcgtaa
     5341 tcttcatgta gttttcacaa tgaatccagc aagtccagat ttccacaatc gttcagctac
     5401 ctctcctgca cttttcaatc gttgtgtttt ggattggttt ggtgaatggt caccagaagc
     5461 tttattccaa gttggttctg aattcactag aaatctcgat cttgaaaatc cacaatacat
     5521 tgctccacca gttttcattc aagaagcaga gataatgggt aataatttaa tggccatccc
     5581 accatcacat cgtgatgcag tcgtaagttc attggtttat attcatcaaa ccattggtga
     5641 agccaatatt cgtttactta aacgtcaagg tcgtcaaaat tatgtaactc cacgtcatta
     5701 tttggatttc attaatcaag tggtattatt aatcaatgag aaacgtgatc aattagaaga
     5761 ggaacaactt catctcaata ttggtcttaa gaaattacgt gatactgagg ctcaagttaa
     5821 agatcttcaa gttagtttag ctcaaaagaa tcgtgaactc gatgttaaga atgaacaagc
     5881 caatcaaaaa ttaaaacaaa tggttcaaga tcaacaagca gctgaaatta aacaaaagga
     5941 tgctcgtgaa cttcaagttc aattggatgt tagaaacaaa gagattgccg ttcaaaaagt
     6001 taaagcttat gcagatttag aaaaagctga accaaccggt ccattacgtg aagaggttga
     6061 acaattagaa aacgctgcaa atgaattgaa attgaaacaa gatgaaatcg ttgccaccat
     6121 tactgccttg gagaaatcta ttgccaccta taaggaagag tatgcaactt taattcgtga
     6181 aactgaacaa attaaaaccg aatcaagtaa agtcaagaat aaggtcgatc gttcaattgc
     6241 tcttttagat aatcttaact ctgaacgtgg tagatgggaa caacaaagtg aaaactttaa
     6301 cactcaaatg tcaactgttg ttggtgatgt tgttttagca tctgctttcc ttgcatatat
     6361 tggtttcttt gatcaaaact tccgtactga ccttatgaga aaatggatga ttcgtttaga
     6421 ttcagttggt attaaattca agagtgatct atcagtacca tctttccttt ccaaaccaga
     6481 agaacgtctt aactggcatg caaattcttt accatccgat gaattatgta ttgaaaatgc
     6541 aatcatgctt aaacgtttca atcgttatcc attggttata gatccatctg gtcaagccat
     6601 ggaattcctt atgaatcaat atgccgacaa aaagattacc aaaactagtt tcctcgattc
     6661 atcatttatg aagaatttag agagtgctct cagatttggt tgtccattat tggtacaaga
     6721 tgttgaaaac attgatccag tattgaatcc agtactcaat aaagaaattc gtaagaaagg
     6781 tggtcgtatt ctcattcgtt taggtgatca agatgtcgat ttctctccat cctttatgat
     6841 ctttttgttc actcgtgacc caaccgctca ctttactcca gatctttgtt ctcgtgttac
     6901 ctttgtaaat ttcactgtca ctccatcctc acttcaaagt caatgtcttc atgaagctct
     6961 taaaactgaa agaccagata ctcataagaa acgttctgat ctcctcaaga ttcaaggtga
     7021 attccaagtt aaattaagaa ttctcgaaaa atctttattg aatgctcttt cccaagcctc
     7081 tggtaacatt ttagatgatg attctgtcat ttcaaccctt gaaacattaa agaaagaaac
     7141 cactgaaatt gcacttaaag ttgaagaaac cgagaccgtt atgcaagaaa ttagtgaagt
     7201 ttcagctctt tataatccaa tggctctctc atgttcacgt gtttactttg caatggaaga
     7261 actttctcaa ttccatctct accaattctc tttacgtgct ttcttggata tcttttataa
     7321 tctacttaat aataatccaa atttagtcga taaaaaagat ccaaatgaac gtttggttta
     7381 tttatcaaaa gatattttct caatgacttt caatcgtgta actcgtacac tcttaaatga
     7441 tgataaatta acatttgcac ttcaattaac tatcatttca gttaaaggta catccaatga
     7501 aatcgaagaa agtgaatggg acttcctttt gaaaggtggt gataatttaa catcaatcaa
     7561 agaaaccatt ccacaattgg attcattatt atcaactact caacaaaaat ggttaatttg
     7621 tcttagacaa caagttccat cattctctaa attggttgat catattcaac aaaactcttc
     7681 cgattggaaa caattctttg gtaaagatca agttggtgaa ccaatcattc cagaatcttg
     7741 gatcgtagct caagctcaac tttctaatca acaatcaacc attgtttcaa actttagaaa
     7801 aattctttta atgaaagctt tccatagtga tagagtttta caatattctc atagttttgt
     7861 ttgttcagta tttggtgaag atttcttaaa tactcaagaa ttagatatgg caaacattgt
     7921 agagaaagag gttaaatctt catcaccatt gttattatgt tcagttccag gttatgatgc
     7981 atcaagtaaa gtcgatgatt tagcattaca attacataaa caatataagt catttgctat
     8041 tggttcacct gaaggttttg aattagccga gaaatcaatt tatgccgccg ctaaatctgg
     8101 tacttgggta ttattaaaga atattcattt ggcaccacaa tggttggtac aattggaaaa
     8161 gaaacttcat tcactctctc cacatccaag ctttagatta tttatgacct ctgaaattca
     8221 tccagctctt ccagcaaatc ttcttcgtat gtcaaatgta ttctcttatg aaaatccacc
     8281 aggtgttaaa gctaatcttt tacatacctt tattggtatt cctgcaactc gtatggataa
     8341 acaaccagca gaacgttcac gtatctattt ccttttagct tggttccatg caatcattca
     8401 agaaagactt cgttatattc cattgggttg gactaaattc tttgaattca atgatgccga
     8461 tttacgtggt gccctagatt caatcgatta ttgggttgat ctctatagta aaggtagaag
     8521 taacattgat ccagacaaga ttccatggat cgctgttcgt accattttag gtagtacaat
     8581 ttatggtggt agaattgata atgaatttga tatgcgttta ctttattcat tccttgaaca
     8641 actttttact ccaagtgcat tcaatccaga tttcccatta gtaccatcca ttggtttatc
     8701 agttccagaa ggtacaactc gtgctcactt tatgaaatgg attgaagctt taccagagat
     8761 ttcaactcca atttggttag gtttacctga aaatgctgaa tcattactct tgtcaaataa
     8821 agctagaaag atgattaatg atctccaaaa aatgcaatca tctgaagaag atggtgaaga
     8881 tgatcaagtt agtggtagtt caaagaaaga atcatcatca tcatcatcag aagataaagg
     8941 taaagctaaa cttagagcaa ccatcactga atggactaaa ctcttaccaa aaccattgaa
     9001 acaattaaag agaaccactc aaaatattaa agatccatta ttccgttgct ttgaaagaga
     9061 aatttcaact ggtggtaaat tagttaaaaa gattaccaat gatctcgcaa atcttttaga
     9121 actaatttct ggtaatatta aatcaaccaa ttatcttcgt tcacttacca cttcaatctc
     9181 taaaggtatc gttccaaaag aatggaaatg gtattcagta cctgaaacca ttagtctttc
     9241 agtttggatc tctgatttct caaagagaat gcaacaatta tcagagattt cagaaagttc
     9301 agattatagt tccattcaag tttggttggg tggtttatta aatccagaag cttacattac
     9361 cgctactaga caatccgctt ctcaattgaa tggttggtcc ttagaaaacc ttagattaca
     9421 cgcttcctca ttgggtaaaa tctcttcaga aggtggtgct agtttcaatg ttaaaggtat
     9481 ggctttagaa ggtgctgttt ggaacaatga tcaactcaca ccaaccgata tcctctcaac
     9541 tccaatttca atcgctactt taacttggaa agataaagat gatccaatct ttaacaattc
     9601 atcttcaaaa ctatctgtac cagtctatct caatgaaact agatcggaat tattattctc
     9661 tatagattta ccatatgatc aatcaacttc aaaacaaaat tggtatcaaa gatcagtttc
     9721 aatcagttca tggaaatctg atatttaaga gctcacgcgt
//
