LOCUS       sequence-469545-        1263 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of low affinity green fluorescent pyruvate 
            biosensor GreenPy1Low.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Imai S, Hario S, Suerte C, Yamaguchi I, Sakoi K, Terai T, 
            Takahashi-Yamashiro K, Campbell RE
  TITLE     High-performance genetically-encoded green and red fluorescent 
            biosensors for pyruvate
  JOURNAL   bioRxiv 2025.04.17.649293
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.04.17.649293"
COMMENT     Sequence Label: pcDNA-GreenPy1Low
FEATURES             Location/Qualifiers
     source          1..1263
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     549..570
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(676..697)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(937..956)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atggtgcaat ctttttccga tccgctggta gaactgcttc cagatcaccc ggaatcccaa
       61 tatgacttgc tcgaagcgcg ccatgcgctg gaagggatcg cagcatatta cgctgccttg
      121 cgcagcacag atgaagataa agagaggatc agagagttgc atcatgcgat tgagcttgcc
      181 caacaaagtg gtgatctcga tgccgaaagt aacgctgtac ttcagtatca aaccgcggtg
      241 accgaggcag cgcacaatgt ggtccttttg cacttgttga gatgtatgga gcctatgctc
      301 gcacagaata tccgacaaaa ctttgagctg ctgtaccgtc ataacgtcta tgtcatggcc
      361 gacaagcaga ggaacggcat caaggcgaac ttcaagatcc gccacaacat cgagggcggc
      421 ggcgtgcagc tcgcctacca ccaccagcag aacaccccca tcggcgacgg ccccgtgctg
      481 ctgcccgaca accactacct gtgcacccag tccaaacttt cgaaagaccc caacgagaag
      541 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
      601 gagctgtaca tgggcggtac cggagggagc atggtgagca agggcgagga gctgttcacc
      661 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      721 tccggcgagg gtgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
      781 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
      841 tgcttcggcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
      901 gaaggctaca tccaggagcg caccatcttc ttcgaggacg acggcaacta caagacccgc
      961 gccgaggtga agttcgaggg cgacaccctg gtgagccgca tcgagctgaa gggcatcgac
     1021 ttcaaggagg acggcaacat cctggggcat aagctggagt acaactcgtg gcgacgagag
     1081 atgctgccgc tcgtgtcttc ccatcgcact cgcatatttg aagctattat ggcaggaaaa
     1141 ccggaggaag ccagggaagc gtcccataaa cacttggcct ttattgagga aatcctgctg
     1201 gaccggtcaa gggaggaatc acgccgcgaa cgctcattga gacggctgga gcaaagaaag
     1261 aat
//
