LOCUS       sequence-469553-        1272 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of high affinity red fluorescent pyruvate 
            biosensor ApplePy1High.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Imai S, Hario S, Suerte C, Yamaguchi I, Sakoi K, Terai T, 
            Takahashi-Yamashiro K, Campbell RE
  TITLE     High-performance genetically-encoded green and red fluorescent 
            biosensors for pyruvate
  JOURNAL   bioRxiv 2025.04.17.649293
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.04.17.649293"
COMMENT     Sequence Label: pcDNA-ApplePy1High
FEATURES             Location/Qualifiers
     source          1..1272
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggtgcaat ctttctccga tccgctgcta gaactgcttt ccgatcaccc ggaatcccaa
       61 tatgacttgc tcgaaacgcg ccatgcgctg gaagggatcg cagcatatta cgctgccttg
      121 tgcagctcag atgaagagaa agagaggatc agagagttgc atcatgcgat tgagcttgcc
      181 caacaaagtg gtgatctcga tgccgaaagt aacgctgtac ttcagtatca aatcgcggtg
      241 accgaggcag cgcacaatgt ggtccttttg cacttgctga gatatatgga gcctatgctc
      301 gcacagaata tccgacataa ctttgagctg ctgtactctg gtacggtggt ttccgagcgg
      361 atgttccccg aggacggcgc cctgaagagc gaggtcaaga aggggctgag gctgaaggac
      421 ggcggccact acgccgtgga ggtcaagacc acctacaagg ccaagaagcc cgtgcccctg
      481 cccggcgcct acatcgtcga catcatgttg gacatcgtgt cccacaacga ggactacact
      541 atcgtggaac agttcgaacg cgccgagggc cgccacccca ccggcggcct ggacgagctg
      601 tacaagggag gtacaggcgg gagtctggta agcaagggcg aggaggatga catggccatc
      661 gtcaaggagt tcatgcgctt caaggtgcac atggagggct ccgttaacgg ccacgagttc
      721 gagatcgaag gcgagggcga gggccgcccc tacgaggcct ttcagactgc taagctgaag
      781 gtgaccaagg gtggccccct gcccttcgcc tgggacatct tatcccctca gttcatgtac
      841 ggctccaagg cctacattaa gcacccagcc gatatccccg actacttcaa gctgtccttc
      901 cccgagggct tcagctggga gcgcgtgatg tacttcgagg acggcggcat tattcacgtt
      961 aaccaggact cctccctgca ggacggcgta tttatctaca aggtgaagct gcgcggcacc
     1021 aacttccccc ccgacggccc cgtaatgcag atgaagacca tgggctggga gtcgggttgg
     1081 cgacgagaga tgctgccgct cgtggcctcc catcgcactc gcatatttga agctattatg
     1141 gcaggaaaac cggaggaagc cagggaagcg tcacatagac acttggcctt tattaaggaa
     1201 atcctgctgg accggtcaag gggggaatca cgccgcgaac tctcattgag aaggctggag
     1261 caaagagaga at
//
