LOCUS       sequence-469583-        5997 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  encodes the orphan human GPR150 receptor, untagged.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5997)
  TITLE     Gruber lab plasmids 2025
REFERENCE   2  (bases 1 to 5997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GPR150-untagged
FEATURES             Location/Qualifiers
     source          1..5997
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1937..1946
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1943..1945,1946..1948,1949..2662)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1943..1945,1946..1948,1949..2659)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1988..2009)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2249..2268)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2596..2617
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2785..2906
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2822..2841)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2876..2895
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2913..3368)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3050..3071)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3262..3281
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3395..3499
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3501..3858
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3501..3692
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3524..3544)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3709..3844
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3893..4687
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3893..4687
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3947..3966)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4557..4576
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4875..4894)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4919..4966
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5295..5883
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5784..5803
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagat ggaggatctg ttctctccgt ccattcttcc tcctgcaccc
      661 aatatatccg tccctattct gttgggttgg ggtttgaacc tgacactggg gcagggagct
      721 cctgcgagtg gaccccccag tcgcagagtc agactggttt tcctcggcgt gatcctggtg
      781 gtagcggtag ccggcaatac tactgttttg tgccgacttt gtggcggcgg aggcccgtgg
      841 gctgggccta aacgccggaa aatggacttc cttctggtgc agttggcttt ggccgatctg
      901 tacgcctgcg ggggtaccgc cctgagtcag ttggcctggg aactgctggg cgaaccacgg
      961 gccgccactg gggatttggc ctgccggttc cttcagttgc tccaagcctc tggcagaggg
     1021 gcctccgccc acctggtggt tttgatagcg ctcgaacggc gccgggccgt gcggctgcct
     1081 cacggaagac cacttcccgc cagagcgctg gctgcgctcg gctggttgct cgctctgttg
     1141 ttggccctgc cccctgcctt tgtggtgcgc ggagactccc ctagcccatt gccgccccct
     1201 ccaccaccaa ctagcctgca acctggtgca ccccctgcgg ctagggcttg gccaggcgag
     1261 agacggtgtc acggcatctt tgcccctctg cccagatggc atcttcaggt gtacgcattt
     1321 tatgaggcag tggccggctt cgtggcccca gtcacagtgc tgggcgtcgc ctgcggccat
     1381 ctcctcagtg tctggtggcg ccaccggcct caggccccgg cggcagccgc tccctggtcc
     1441 gctagccccg gcagggcacc cgccccttct gcgctgcccc gggccaaggt ccagagcctc
     1501 aagatgagct tgctgctggc ccttcttttt gtcgggtgtg agcttcccta cttcgctgct
     1561 agactggccg ccgcctggtc aagcgggcct gctggagact gggaaggcga aggcttgagc
     1621 gctgcgctta gagtcgtcgc catggcaaac tcagctctga acccatttgt gtacctgttc
     1681 ttccaggcag gagattgtag gcttaggagg cagctgcgca agagactcgg aagcttgtgt
     1741 tgtgcaccac aaggtggtgc cgaagacgag gagggcccaa gagggcacca ggcactctac
     1801 cgccaaaggt ggccgcaccc gcactatcat catgcacgga gagaacccct ggacgagggc
     1861 ggtctgaggc ctccaccacc aaggcccagg ccactgccat gttcctgtga gtcagctttc
     1921 taaggatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     1981 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     2041 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     2101 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     2161 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     2221 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     2281 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     2341 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     2401 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     2461 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     2521 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     2581 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     2641 catggacgag ctgtacaagt aaagcggccg cgactctaga tcataatcag ccataccaca
     2701 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     2761 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     2821 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     2881 ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa
     2941 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     3001 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     3061 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     3121 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     3181 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     3241 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     3301 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     3361 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     3421 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     3481 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
     3541 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     3601 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     3661 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     3721 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     3781 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     3841 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga
     3901 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     3961 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     4021 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
     4081 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     4141 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     4201 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     4261 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     4321 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     4381 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
     4441 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     4501 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     4561 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     4621 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     4681 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     4741 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     4801 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct
     4861 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
     4921 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt
     4981 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
     5041 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
     5101 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt
     5161 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     5221 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     5281 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     5341 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     5401 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     5461 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     5521 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     5581 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     5641 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     5701 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     5761 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     5821 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     5881 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     5941 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat
//
