LOCUS       sequence-469588-       10409 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for PiggyBac mediated integration and stable expression of hFGFR2b 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10409)
  AUTHORS   Zlinska V, Feketova Z, Czyrek A, Chudzian J, Zivkovic ML, Ursachi 
            VC, Dudeja P, Fafilek B, Rynes J, Rico-Llanos G, Koudelka A, Roy T, 
            Biadun M, Raskova V, Svozilova K, Stroblova M, Krzyscik M, Hristova 
            K, Krowarsch D, Foldynova-Trantirkova S, Zakrzewska M, Trantirek L, 
            Krejci P
  TITLE     Specific inhibition of fibroblast growth factor receptor 1 signaling
            by a DNA aptamer.
  JOURNAL   Mol Ther Nucleic Acids. 2024 Nov 28;36(1):102405. doi: 
            10.1016/j.omtn.2024.102405. eCollection 2025 Mar 11.
  PUBMED    39759879
REFERENCE   2  (bases 1 to 10409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Nucleic Acids."; date: "2024-11-28"; volume: "36(1):102405. doi"; 
            pages: " 10.1016/j.omtn.2024.102405. eCollection 2025 Mar 11"
COMMENT     Sequence Label: pTR01F/hFGFR2b-V5
FEATURES             Location/Qualifiers
     source          1..10409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     1421..1440
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        1574..1777
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1728..1748
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1822..1841
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1822..1840
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2294..2305
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     CDS             4439..4480
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4490..4507
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4558..4679
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4927..5138
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             5151..5419
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      5448..5457
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5478..6140
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             6264..6863
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6264..6283)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6760..6780
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(6917..6937)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    6979..7100
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   7863..7897
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8388..8404)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8388..8404)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8401..8423)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8412..8428
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8436..8442,8443..8460,8461..8466))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8481..8502
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8619..8636)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8790..9378)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8870..8889)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9549..10340,10341..10409))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10172..10191
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccacta gtattatgcc cagtacatga
     1381 ccttatggga ctttcctact tggcagtaca tctacgtaaa ctagcaaaat aggctgtccc
     1441 cagtgcaagt gcaggtgcca gaacatttct ctggcctaac tggccggtac ctgagctcgc
     1501 tagcgggaat ttccggggac tttccgggaa tttccgggga ctttccggga atttccagat
     1561 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     1621 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     1681 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     1741 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
     1801 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagtt
     1861 aagcttggta ccgagctcgg atccagtacc cttcaccatg gtcagctggg gtcgtttcat
     1921 ctgcctggtc gtggtcacca tggcaacctt gtccctggcc cggccctcct tcagtttagt
     1981 tgaggatacc acattagagc cagaagagcc accaaccaaa taccaaatct ctcaaccaga
     2041 agtgtacgtg gctgcgccag gggagtcgct agaggtgcgc tgcctgttga aagatgccgc
     2101 cgtgatcagt tggactaagg atggggtgca cttggggccc aacaatagga cagtgcttat
     2161 tggggagtac ttgcagataa agggcgccac gcctagagac tccggcctct atgcttgtac
     2221 tgccagtagg actgtagaca gtgaaacttg gtacttcatg gtgaatgtca cagatgccat
     2281 ctcatccgga gatgatgagg atgacaccga tggtgcggaa gattttgtca gtgagaacag
     2341 taacaacaag agagcaccat actggaccaa cacagaaaag atggaaaagc ggctccatgc
     2401 tgtgcctgcg gccaacactg tcaagtttcg ctgcccagcc gggggaaacc caatgccaac
     2461 catgcggtgg ctgaaaaacg ggaaggagtt taagcaggag catcgcattg gaggctacaa
     2521 ggtacgaaac cagcactgga gcctcattat ggaaagtgtg gtcccatctg acaagggaaa
     2581 ttatacctgt gtggtggaga atgaatacgg gtccatcaat cacacgtacc acctggatgt
     2641 tgtggagcga tcgcctcacc ggcccatcct ccaagccgga ctgccggcaa atgcctccac
     2701 agtggtcgga ggagacgtag agtttgtctg caaggtttac agtgatgccc agccccacat
     2761 ccagtggatc aagcacgtgg aaaagaacgg cagtaaatac gggcccgacg ggctgcccta
     2821 cctcaaggtt ctcaagcact cggggataaa tagttccaat gcagaagtgc tggctctgtt
     2881 caatgtgacc gaggcggatg ctggggaata tatatgtaag gtctccaatt atatagggca
     2941 ggccaaccag tctgcctggc tcactgtcct gccaaaacag caagcgcctg gaagagaaaa
     3001 ggagattaca gcttccccag actacctgga gatagccatt tactgcatag gggtcttctt
     3061 aatcgcctgt atggtggtaa cagtcatcct gtgccgaatg aagaacacga ccaagaagcc
     3121 agacttcagc agccagccgg ctgtgcacaa gctgaccaaa cgtatccccc tgcggagaca
     3181 ggtaacagtt tcggctgagt ccagctcctc catgaactcc aacaccccgc tggtgaggat
     3241 aacaacacgc ctctcttcaa cggcagacac ccccatgctg gcaggggtct ccgagtatga
     3301 acttccagag gacccaaaat gggagtttcc aagagataag ctgacactgg gcaagcccct
     3361 gggagaaggt tgctttgggc aagtggtcat ggcggaagca gtgggaattg acaaagacaa
     3421 gcccaaggag gcggtcaccg tggccgtgaa gatgttgaaa gatgatgcca cagagaaaga
     3481 cctttctgat ctggtgtcag agatggagat gatgaagatg attgggaaac acaagaatat
     3541 cataaatctt cttggagcct gcacacagga tgggcctctc tatgtcatag ttgagtatgc
     3601 ctctaaaggc aacctccgag aatacctccg agcccggagg ccacccggga tggagtactc
     3661 ctatgacatt aaccgtgttc ctgaggagca gatgaccttc aaggacttgg tgtcatgcac
     3721 ctaccagctg gccagaggca tggagtactt ggcttcccaa aaatgtattc atcgagattt
     3781 agcagccaga aatgttttgg taacagaaaa caatgtgatg aaaatagcag actttggact
     3841 cgccagagat atcaacaata tagactatta caaaaagacc accaatgggc ggcttccagt
     3901 caagtggatg gctccagaag ccctgtttga tagagtatac actcatcaga gtgatgtctg
     3961 gtccttcggg gtgttaatgt gggagatctt cactttaggg ggctcgccct acccagggat
     4021 tcccgtggag gaacttttta agctgctgaa ggaaggacac agaatggata agccagccaa
     4081 ctgcaccaac gaactgtaca tgatgatgag ggactgttgg catgcagtgc cctcccagag
     4141 accaacgttc aagcagttgg tagaagactt ggatcgaatc ctcactctca caaccaatga
     4201 ggaatacttg gacctcagcc aacctctcga acagtattca cctagttacc ctgacacaag
     4261 aagttcttgt tcttcaggag atgattctgt tttttctcca gaccccatgc cttacgaacc
     4321 atgccttcct cagtatccac acataaacgg cagtgttaaa acagcgaagg gtcaagacaa
     4381 ttctgcagat atccagcaca gtggcggccg ctcgagtcta gagggcccgc ggttcgaagg
     4441 taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtc atcatcacca
     4501 tcaccattga gtttaaaccc tagagtcggg gcggccggcc gcttcgagca gacatgataa
     4561 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     4621 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     4681 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     4741 aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatccgttt gcgtattggg
     4801 cgctcttccg ctgatctgcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg
     4861 gttagctacc ttctaaatcg gatccgcggc cgcgaaggat ctgcgatcgc tccggtgccc
     4921 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
     4981 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
     5041 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
     5101 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
     5161 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
     5221 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
     5281 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
     5341 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
     5401 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagacgcc accatggaga
     5461 gcgacgagag cggcctgccc gccatggaga tcgagtgccg catcaccggc accctgaacg
     5521 gcgtggagtt cgagctggtg ggcggcggag agggcacccc caagcagggc cgcatgacca
     5581 acaagatgaa gagcaccaaa ggcgccctga ccttcagccc ctacctgctg agccacgtga
     5641 tgggctacgg cttctaccac ttcggcacct accccagcgg ctacgagaac cccttcctgc
     5701 acgccatcaa caacggcggc tacaccaaca cccgcatcga gaagtacgag gacggcggcg
     5761 tgctgcacgt gagcttcagc taccgctacg aggccggccg cgtgatcggc gacttcaagg
     5821 tggtgggcac cggcttcccc gaggacagcg tgatcttcac cgacaagatc atccgcagca
     5881 acgccaccgt ggagcacctg caccccatgg gcgataacgt gctggtgggc agcttcgccc
     5941 gcaccttcag cctgcgcgac ggcggctact acagcttcgt ggtggacagc cacatgcact
     6001 tcaagagcgc catccacccc agcatcctgc agaacggggg ccccatgttc gccttccgcc
     6061 gcgtggagga gctgcacagc aacaccgagc tgggcatcgt ggagtaccag cacgccttca
     6121 agacccccat cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc gtggacggca
     6181 ccgccggacc cggctccacc ggatctcgcg agggcagagg aagtcttcta acatgcggtg
     6241 acgkggagga gaatcccggc cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     6301 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     6361 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     6421 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     6481 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     6541 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     6601 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     6661 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     6721 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     6781 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     6841 tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta caaaatttgt gaaagattga
     6901 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     6961 tgtatcatgc gttaactaaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     7021 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     7081 aaactcatca atgtatctta tcatgtctgg aattgactca aatgatgtca attagtctat
     7141 cagaagctca tctggtctcc cttccggggg acaagacatc cctgtttaat atttaaacag
     7201 cagtgttccc aaactgggtt cttatatccc ttgctctggt caaccaggtt gcagggtttc
     7261 ctgtcctcac aggaacgaag tccctaaaga aacagtggca gccaggttta gccccggaat
     7321 tgactggatt ccttttttag ggcccattgg tatggctttt tccccgtatc cccccaggtg
     7381 tctgcaggct caaagagcag cgagaagcgt tcagaggaaa gcgatcccgt gccaccttcc
     7441 ccgtgcccgg gctgtccccg cacgctgccg gctcggggat gcggggggag cgccggaccg
     7501 gagcggagcc ccgggcggct cgctgctgcc ccctagcggg ggagggacgt aattacatcc
     7561 ctgggggctt tggggggggg ctgtccctga tatctataac aagaaaatat atatataata
     7621 agttatcacg taagtagaac atgaaataac aatataatta tcgtatgagt taaatcttaa
     7681 aagtcacgta aaagataatc atgcgtcatt ttgactcacg cggtcgttat agttcaaaat
     7741 cagtgacact taccgcattg acaagcacgc ctcacgggag ctccaagcgg cgactgagat
     7801 gtcctaaatg cacagcgacg gattcgcgct atttagaaag agagagcaat atttcaagaa
     7861 tgcatgcgtc aattttacgc agactatctt tctagggtta atctagctgc atcaggatca
     7921 tatcgtcggg tcttttttcc ggctcagtca tcgcccaagc tggcgctatc tgggcatcgg
     7981 ggaggaagaa gcccgtgcct tttcccgcga ggttgaagcg gcatggaaag agtttgccga
     8041 ggatgactgc tgctgcattg acgttgagcg aaaacgcacg tttaccatga tgattcggga
     8101 aggtgtggcc atgcacgcct ttaacggtga actgttcgtt caggccacct gggataccag
     8161 ttcgtcgcgg cttttccgga cacagttccg gatggtcagc ccgaagcgca tcagcaaccc
     8221 gaacaatacc ggcgacagcc ggaactgccg tgccggtgtg cagattaatg acagcggtgc
     8281 ggcgctggga tattacgtca gcgaggacgg gtatcctggc tggatgccgc agaaatggac
     8341 atggataccc cgtgagttac ccggcgggcg cgcttggcgt aatcatggtc atagctgttt
     8401 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     8461 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     8521 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     8581 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     8641 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     8701 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     8761 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     8821 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     8881 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     8941 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     9001 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     9061 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     9121 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     9181 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     9241 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     9301 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     9361 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     9421 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     9481 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     9541 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     9601 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     9661 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     9721 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     9781 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     9841 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     9901 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     9961 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    10021 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    10081 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    10141 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    10201 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    10261 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    10321 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    10381 agggcgacac ggaaatgttg aatactcat
//
