LOCUS       sequence-469604-       10379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for PiggyBac mediated integration and stable expression of hFGFR3b 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10379)
  AUTHORS   Zlinska V, Feketova Z, Czyrek A, Chudzian J, Zivkovic ML, Ursachi 
            VC, Dudeja P, Fafilek B, Rynes J, Rico-Llanos G, Koudelka A, Roy T, 
            Biadun M, Raskova V, Svozilova K, Stroblova M, Krzyscik M, Hristova 
            K, Krowarsch D, Foldynova-Trantirkova S, Zakrzewska M, Trantirek L, 
            Krejci P
  TITLE     Specific inhibition of fibroblast growth factor receptor 1 signaling
            by a DNA aptamer.
  JOURNAL   Mol Ther Nucleic Acids. 2024 Nov 28;36(1):102405. doi: 
            10.1016/j.omtn.2024.102405. eCollection 2025 Mar 11.
  PUBMED    39759879
REFERENCE   2  (bases 1 to 10379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Nucleic Acids."; date: "2024-11-28"; volume: "36(1):102405. doi"; 
            pages: " 10.1016/j.omtn.2024.102405. eCollection 2025 Mar 11"
COMMENT     Sequence Label: pTR01F/hFGFR3b-V5
FEATURES             Location/Qualifiers
     source          1..10379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     1421..1440
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        1574..1777
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1728..1748
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1822..1841
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1822..1840
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4409..4450
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4460..4477
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4528..4649
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4897..5108
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             5121..5389
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      5418..5427
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5448..6110
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             6180..6233
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6234..6833
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6234..6253)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6730..6750
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(6887..6907)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    6949..7070
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   7833..7867
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8358..8374)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8358..8374)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8371..8393)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8382..8398
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8406..8412,8413..8430,8431..8436))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8451..8472
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8589..8606)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8760..9348)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8840..8859)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9519..10310,10311..10379))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10142..10161
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccacta gtattatgcc cagtacatga
     1381 ccttatggga ctttcctact tggcagtaca tctacgtaaa ctagcaaaat aggctgtccc
     1441 cagtgcaagt gcaggtgcca gaacatttct ctggcctaac tggccggtac ctgagctcgc
     1501 tagcgggaat ttccggggac tttccgggaa tttccgggga ctttccggga atttccagat
     1561 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     1621 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     1681 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     1741 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
     1801 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagtt
     1861 aagcttggta ccgagctcgg atccagtacc cttcacccac gaccccacca tgggcgcccc
     1921 tgcctgcgcc ctcgcgctct gcgtggccgt ggccatcgtg gccggcgcct cctcggagtc
     1981 cttggggacg gagcagcgcg tcgtggggcg agcggcagaa gtcccgggcc cagagcccgg
     2041 ccagcaggag cagttggtct tcggcagcgg ggatgctgtg gagctgagct gtcccccgcc
     2101 cgggggtggt cccatggggc ccactgtctg ggtcaaggat ggcacagggc tggtgccctc
     2161 ggagcgtgtc ctggtggggc cccagcggct gcaggtgctg aatgcctccc acgaggactc
     2221 cggggcctac agctgccggc agcggctcac gcagcgcgta ctgtgccact tcagtgtgcg
     2281 ggtgacagac gctccatcct cgggagatga cgaagacggg gaggacgagg ctgaggacac
     2341 aggtgtggac acaggggccc cttactggac acggcccgag cggatggaca agaagctgct
     2401 ggccgtgccg gccgccaaca ccgtccgctt ccgctgccca gccgctggca accccactcc
     2461 ctccatctcc tggctgaaga acggcaggga gttccgcggc gagcaccgca ttggaggcat
     2521 caagctgcgg catcagcagt ggagcctggt catggaaagc gtggtgccct cggaccgcgg
     2581 caactacacc tgcgtcgtgg agaacaagtt tggcagcatc cggcagacgt acacgctgga
     2641 cgtgctggag cgctccccgc accggcccat cctgcaggcg gggctgccgg ccaaccagac
     2701 ggcggtgctg ggcagcgacg tggagttcca ctgcaaggtg tacagtgacg cacagcccca
     2761 catccagtgg ctcaagcacg tggaggtgaa cggcagcaag gtgggcccgg acggcacacc
     2821 ctacgttacc gtgctcaagt cctggatcag tgagagtgtg gaggccgacg tgcgcctccg
     2881 cctggccaat gtgtcggagc gggacggggg cgagtacctc tgtcgagcca ccaatttcat
     2941 aggcgtggcc gagaaggcct tttggctgag cgttcacggg ccccgagcag ccgaggagga
     3001 gctggtggag gctgacgagg cgggcagtgt gtatgcaggc atcctcagct acggggtggg
     3061 cttcttcctg ttcatcctgg tggtggcggc tgtgacgctc tgccgcctgc gcagcccccc
     3121 caagaaaggc ctgggctccc ccaccgtgca caagatctcc cgcttcccgc tcaagcgaca
     3181 ggtgtccctg gagtccaacg cgtccatgag ctccaacaca ccactggtgc gcatcgcaag
     3241 gctgtcctca ggggagggcc ccacgctggc caatgtctcc gagctcgagc tgcctgccga
     3301 ccccaaatgg gagctgtctc gggcccggct gaccctgggc aagccccttg gggagggctg
     3361 cttcggccag gtggtcatgg cggaggccat cggcattgac aaggaccggg ccgccaagcc
     3421 tgtcaccgta gccgtgaaga tgctgaaaga cgatgccact gacaaggacc tgtcggacct
     3481 ggtgtctgag atggagatga tgaagatgat cgggaaacac aaaaacatca tcaacctgct
     3541 gggcgcctgc acgcagggcg ggcccctgta cgtgctggtt gagtacgcgg ccaagggtaa
     3601 cctgcgggag tttctgcggg cgcggcggcc cccgggcctg gactactcct tcgacacctg
     3661 caagccgccc gaggagcagc tcaccttcaa ggacctggtg tcctgtgcct accaggtggc
     3721 ccggggcatg gagtacttgg cctcccagaa gtgcatccac agggacctgg ctgcccgcaa
     3781 tgtgctggtg accgaggaca acgtgatgaa gatcgcagac ttcgggctgg cccgggacgt
     3841 gcacaacctc gactactaca agaagacaac caacggccgg ctgcccgtga agtggatggc
     3901 gcctgaggcc ttgtttgacc gagtctacac tcaccagagt gacgtctggt cctttggggt
     3961 cctgctctgg gagatcttca cgctgggggg ctccccgtac cccggcatcc ctgtggagga
     4021 gctcttcaag ctgctgaagg agggccaccg catggacaag cccgccaact gcacacacga
     4081 cctgtacatg atcatgcggg agtgctggca tgccgcgccc tcccagaggc ccaccttcaa
     4141 gcagctggtg gaggacctgg accgtgtcct taccgtgacg tccaccgacg agtacctgga
     4201 cctgtcggcg cctttcgagc agtactcccc gggtggccag gacaccccca gctccagctc
     4261 ctcaggggac gactccgtgt ttgcccacga cctgctgccc ccggccccac ccagcagtgg
     4321 gggctcgcgg acggccaagg gtcaagacaa ttctgcagat atccagcaca gtggcggccg
     4381 ctcgagtcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct
     4441 cgattctacg cgtaccggtc atcatcacca tcaccattga gtttaaaccc tagagtcggg
     4501 gcggccggcc gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa
     4561 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     4621 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     4681 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     4741 aaatcgataa ggatccgttt gcgtattggg cgctcttccg ctgatctgcg cagcaccatg
     4801 gcctgaaata acctctgaaa gaggaacttg gttagctacc ttctaaatcg gatccgcggc
     4861 cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
     4921 tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg
     4981 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     5041 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     5101 gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct
     5161 gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct
     5221 gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg
     5281 cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg
     5341 ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac
     5401 cggcgcctac gctagacgcc accatggaga gcgacgagag cggcctgccc gccatggaga
     5461 tcgagtgccg catcaccggc accctgaacg gcgtggagtt cgagctggtg ggcggcggag
     5521 agggcacccc caagcagggc cgcatgacca acaagatgaa gagcaccaaa ggcgccctga
     5581 ccttcagccc ctacctgctg agccacgtga tgggctacgg cttctaccac ttcggcacct
     5641 accccagcgg ctacgagaac cccttcctgc acgccatcaa caacggcggc tacaccaaca
     5701 cccgcatcga gaagtacgag gacggcggcg tgctgcacgt gagcttcagc taccgctacg
     5761 aggccggccg cgtgatcggc gacttcaagg tggtgggcac cggcttcccc gaggacagcg
     5821 tgatcttcac cgacaagatc atccgcagca acgccaccgt ggagcacctg caccccatgg
     5881 gcgataacgt gctggtgggc agcttcgccc gcaccttcag cctgcgcgac ggcggctact
     5941 acagcttcgt ggtggacagc cacatgcact tcaagagcgc catccacccc agcatcctgc
     6001 agaacggggg ccccatgttc gccttccgcc gcgtggagga gctgcacagc aacaccgagc
     6061 tgggcatcgt ggagtaccag cacgccttca agacccccat cgccttcgcc agatcccgcg
     6121 ctcagtcgtc caattctgcc gtggacggca ccgccggacc cggctccacc ggatctcgcg
     6181 agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc cctatgaccg
     6241 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     6301 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     6361 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     6421 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     6481 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     6541 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     6601 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     6661 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     6721 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     6781 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaaatcaac
     6841 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     6901 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc gttaactaaa cttgtttatt
     6961 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     7021 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     7081 aattgactca aatgatgtca attagtctat cagaagctca tctggtctcc cttccggggg
     7141 acaagacatc cctgtttaat atttaaacag cagtgttccc aaactgggtt cttatatccc
     7201 ttgctctggt caaccaggtt gcagggtttc ctgtcctcac aggaacgaag tccctaaaga
     7261 aacagtggca gccaggttta gccccggaat tgactggatt ccttttttag ggcccattgg
     7321 tatggctttt tccccgtatc cccccaggtg tctgcaggct caaagagcag cgagaagcgt
     7381 tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg cacgctgccg
     7441 gctcggggat gcggggggag cgccggaccg gagcggagcc ccgggcggct cgctgctgcc
     7501 ccctagcggg ggagggacgt aattacatcc ctgggggctt tggggggggg ctgtccctga
     7561 tatctataac aagaaaatat atatataata agttatcacg taagtagaac atgaaataac
     7621 aatataatta tcgtatgagt taaatcttaa aagtcacgta aaagataatc atgcgtcatt
     7681 ttgactcacg cggtcgttat agttcaaaat cagtgacact taccgcattg acaagcacgc
     7741 ctcacgggag ctccaagcgg cgactgagat gtcctaaatg cacagcgacg gattcgcgct
     7801 atttagaaag agagagcaat atttcaagaa tgcatgcgtc aattttacgc agactatctt
     7861 tctagggtta atctagctgc atcaggatca tatcgtcggg tcttttttcc ggctcagtca
     7921 tcgcccaagc tggcgctatc tgggcatcgg ggaggaagaa gcccgtgcct tttcccgcga
     7981 ggttgaagcg gcatggaaag agtttgccga ggatgactgc tgctgcattg acgttgagcg
     8041 aaaacgcacg tttaccatga tgattcggga aggtgtggcc atgcacgcct ttaacggtga
     8101 actgttcgtt caggccacct gggataccag ttcgtcgcgg cttttccgga cacagttccg
     8161 gatggtcagc ccgaagcgca tcagcaaccc gaacaatacc ggcgacagcc ggaactgccg
     8221 tgccggtgtg cagattaatg acagcggtgc ggcgctggga tattacgtca gcgaggacgg
     8281 gtatcctggc tggatgccgc agaaatggac atggataccc cgtgagttac ccggcgggcg
     8341 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     8401 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     8461 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     8521 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     8581 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     8641 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     8701 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     8761 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     8821 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     8881 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     8941 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     9001 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     9061 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     9121 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     9181 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     9241 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     9301 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     9361 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     9421 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     9481 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     9541 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     9601 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     9661 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     9721 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     9781 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     9841 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     9901 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     9961 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    10021 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    10081 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    10141 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    10201 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    10261 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    10321 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcat
//
