LOCUS       sequence-469608-       10406 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for PiggyBac mediated integration and stable expression of hFGFR2c 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10406)
  AUTHORS   Zlinska V, Feketova Z, Czyrek A, Chudzian J, Zivkovic ML, Ursachi 
            VC, Dudeja P, Fafilek B, Rynes J, Rico-Llanos G, Koudelka A, Roy T, 
            Biadun M, Raskova V, Svozilova K, Stroblova M, Krzyscik M, Hristova 
            K, Krowarsch D, Foldynova-Trantirkova S, Zakrzewska M, Trantirek L, 
            Krejci P
  TITLE     Specific inhibition of fibroblast growth factor receptor 1 signaling
            by a DNA aptamer.
  JOURNAL   Mol Ther Nucleic Acids. 2024 Nov 28;36(1):102405. doi: 
            10.1016/j.omtn.2024.102405. eCollection 2025 Mar 11.
  PUBMED    39759879
REFERENCE   2  (bases 1 to 10406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Nucleic Acids."; date: "2024-11-28"; volume: "36(1):102405. doi"; 
            pages: " 10.1016/j.omtn.2024.102405. eCollection 2025 Mar 11"
COMMENT     Sequence Label: pTR01F/hFGFR2c-V5
FEATURES             Location/Qualifiers
     source          1..10406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     1421..1440
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        1574..1777
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1728..1748
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1822..1841
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1822..1840
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2294..2305
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     CDS             4436..4477
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4487..4504
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4555..4676
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4924..5135
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             5148..5416
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      5445..5454
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5475..6137
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             6207..6260
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6261..6860
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6261..6280)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6757..6777
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(6914..6934)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    6976..7097
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   7860..7894
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8385..8401)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8385..8401)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8398..8420)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8409..8425
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8433..8439,8440..8457,8458..8463))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8478..8499
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8616..8633)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8787..9375)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8867..8886)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9546..10337,10338..10406))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10169..10188
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccacta gtattatgcc cagtacatga
     1381 ccttatggga ctttcctact tggcagtaca tctacgtaaa ctagcaaaat aggctgtccc
     1441 cagtgcaagt gcaggtgcca gaacatttct ctggcctaac tggccggtac ctgagctcgc
     1501 tagcgggaat ttccggggac tttccgggaa tttccgggga ctttccggga atttccagat
     1561 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     1621 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     1681 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     1741 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
     1801 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagtt
     1861 aagcttggta ccgagctcgg atccagtacc cttcaccatg gtcagctggg gtcgtttcat
     1921 ctgcctggtc gtggtcacca tggcaacctt gtccctggcc cggccctcct tcagtttagt
     1981 tgaggatacc acattagagc cagaagagcc accaaccaaa taccaaatct ctcaaccaga
     2041 agtgtacgtg gctgcgccag gggagtcgct agaggtgcgc tgcctgttga aagatgccgc
     2101 cgtgatcagt tggactaagg atggggtgca cttggggccc aacaatagga cagtgcttat
     2161 tggggagtac ttgcagataa agggcgccac gcctagagac tccggcctct atgcttgtac
     2221 tgccagtagg actgtagaca gtgaaacttg gtacttcatg gtgaatgtca cagatgccat
     2281 ctcatccgga gatgatgagg atgacaccga tggtgcggaa gattttgtca gtgagaacag
     2341 taacaacaag agagcaccat actggaccaa cacagaaaag atggaaaagc ggctccatgc
     2401 tgtgcctgcg gccaacactg tcaagtttcg ctgcccagcc gggggaaacc caatgccaac
     2461 catgcggtgg ctgaaaaacg ggaaggagtt taagcaggag catcgcattg gaggctacaa
     2521 ggtacgaaac cagcactgga gcctcattat ggaaagtgtg gtcccatctg acaagggaaa
     2581 ttatacctgt gtggtggaga atgaatacgg gtccatcaat cacacgtacc acctggatgt
     2641 tgtggagcga tcgcctcacc ggcccatcct ccaagccgga ctgccggcaa atgcctccac
     2701 agtggtcgga ggagacgtag agtttgtctg caaggtttac agtgatgccc agccccacat
     2761 ccagtggatc aagcacgtgg aaaagaacgg cagtaaatac gggcccgacg ggctgcccta
     2821 cctcaaggtt ctcaaggccg ccggtgttaa caccacggac aaagagattg aggttctcta
     2881 tattcggaat gtaacttttg aggacgctgg ggaatatacg tgcttggcgg gtaattctat
     2941 tgggatatcc tttcactctg catggttgac agttctgcca gcgcctggaa gagaaaagga
     3001 gattacagct tccccagact acctggagat agccatttac tgcatagggg tcttcttaat
     3061 cgcctgtatg gtggtaacag tcatcctgtg ccgaatgaag aacacgacca agaagccaga
     3121 cttcagcagc cagccggctg tgcacaagct gaccaaacgt atccccctgc ggagacaggt
     3181 aacagtttcg gctgagtcca gctcctccat gaactccaac accccgctgg tgaggataac
     3241 aacacgcctc tcttcaacgg cagacacccc catgctggca ggggtctccg agtatgaact
     3301 tccagaggac ccaaaatggg agtttccaag agataagctg acactgggca agcccctggg
     3361 agaaggttgc tttgggcaag tggtcatggc ggaagcagtg ggaattgaca aagacaagcc
     3421 caaggaggcg gtcaccgtgg ccgtgaagat gttgaaagat gatgccacag agaaagacct
     3481 ttctgatctg gtgtcagaga tggagatgat gaagatgatt gggaaacaca agaatatcat
     3541 aaatcttctt ggagcctgca cacaggatgg gcctctctat gtcatagttg agtatgcctc
     3601 taaaggcaac ctccgagaat acctccgagc ccggaggcca cccgggatgg agtactccta
     3661 tgacattaac cgtgttcctg aggagcagat gaccttcaag gacttggtgt catgcaccta
     3721 ccagctggcc agaggcatgg agtacttggc ttcccaaaaa tgtattcatc gagatttagc
     3781 agccagaaat gttttggtaa cagaaaacaa tgtgatgaaa atagcagact ttggactcgc
     3841 cagagatatc aacaatatag actattacaa aaagaccacc aatgggcggc ttccagtcaa
     3901 gtggatggct ccagaagccc tgtttgatag agtatacact catcagagtg atgtctggtc
     3961 cttcggggtg ttaatgtggg agatcttcac tttagggggc tcgccctacc cagggattcc
     4021 cgtggaggaa ctttttaagc tgctgaagga aggacacaga atggataagc cagccaactg
     4081 caccaacgaa ctgtacatga tgatgaggga ctgttggcat gcagtgccct cccagagacc
     4141 aacgttcaag cagttggtag aagacttgga tcgaatcctc actctcacaa ccaatgagga
     4201 atacttggac ctcagccaac ctctcgaaca gtattcacct agttaccctg acacaagaag
     4261 ttcttgttct tcaggagatg attctgtttt ttctccagac cccatgcctt acgaaccatg
     4321 ccttcctcag tatccacaca taaacggcag tgttaaaaca gcgaagggtc aagacaattc
     4381 tgcagatatc cagcacagtg gcggccgctc gagtctagag ggcccgcggt tcgaaggtaa
     4441 gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggtcatc atcaccatca
     4501 ccattgagtt taaaccctag agtcggggcg gccggccgct tcgagcagac atgataagat
     4561 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     4621 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     4681 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
     4741 gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tccgtttgcg tattgggcgc
     4801 tcttccgctg atctgcgcag caccatggcc tgaaataacc tctgaaagag gaacttggtt
     4861 agctaccttc taaatcggat ccgcggccgc gaaggatctg cgatcgctcc ggtgcccgtc
     4921 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     4981 gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     5041 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     5101 ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct
     5161 ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc
     5221 tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc
     5281 tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc
     5341 tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc
     5401 tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc atggagagcg
     5461 acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc ctgaacggcg
     5521 tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc atgaccaaca
     5581 agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc cacgtgatgg
     5641 gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc ttcctgcacg
     5701 ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc
     5761 tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg
     5821 tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc cgcagcaacg
     5881 ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca
     5941 ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac atgcacttca
     6001 agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc ttccgccgcg
     6061 tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac gccttcaaga
     6121 cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg
     6181 ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg
     6241 tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     6301 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     6361 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     6421 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     6481 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     6541 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     6601 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     6661 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     6721 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     6781 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     6841 cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa agattgactg
     6901 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     6961 atcatgcgtt aactaaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     7021 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     7081 ctcatcaatg tatcttatca tgtctggaat tgactcaaat gatgtcaatt agtctatcag
     7141 aagctcatct ggtctccctt ccgggggaca agacatccct gtttaatatt taaacagcag
     7201 tgttcccaaa ctgggttctt atatcccttg ctctggtcaa ccaggttgca gggtttcctg
     7261 tcctcacagg aacgaagtcc ctaaagaaac agtggcagcc aggtttagcc ccggaattga
     7321 ctggattcct tttttagggc ccattggtat ggctttttcc ccgtatcccc ccaggtgtct
     7381 gcaggctcaa agagcagcga gaagcgttca gaggaaagcg atcccgtgcc accttccccg
     7441 tgcccgggct gtccccgcac gctgccggct cggggatgcg gggggagcgc cggaccggag
     7501 cggagccccg ggcggctcgc tgctgccccc tagcggggga gggacgtaat tacatccctg
     7561 ggggctttgg gggggggctg tccctgatat ctataacaag aaaatatata tataataagt
     7621 tatcacgtaa gtagaacatg aaataacaat ataattatcg tatgagttaa atcttaaaag
     7681 tcacgtaaaa gataatcatg cgtcattttg actcacgcgg tcgttatagt tcaaaatcag
     7741 tgacacttac cgcattgaca agcacgcctc acgggagctc caagcggcga ctgagatgtc
     7801 ctaaatgcac agcgacggat tcgcgctatt tagaaagaga gagcaatatt tcaagaatgc
     7861 atgcgtcaat tttacgcaga ctatctttct agggttaatc tagctgcatc aggatcatat
     7921 cgtcgggtct tttttccggc tcagtcatcg cccaagctgg cgctatctgg gcatcgggga
     7981 ggaagaagcc cgtgcctttt cccgcgaggt tgaagcggca tggaaagagt ttgccgagga
     8041 tgactgctgc tgcattgacg ttgagcgaaa acgcacgttt accatgatga ttcgggaagg
     8101 tgtggccatg cacgccttta acggtgaact gttcgttcag gccacctggg ataccagttc
     8161 gtcgcggctt ttccggacac agttccggat ggtcagcccg aagcgcatca gcaacccgaa
     8221 caataccggc gacagccgga actgccgtgc cggtgtgcag attaatgaca gcggtgcggc
     8281 gctgggatat tacgtcagcg aggacgggta tcctggctgg atgccgcaga aatggacatg
     8341 gataccccgt gagttacccg gcgggcgcgc ttggcgtaat catggtcata gctgtttcct
     8401 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     8461 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     8521 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     8581 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8641 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8701 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8761 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8821 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8881 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8941 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     9001 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     9061 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     9121 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     9181 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     9241 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     9301 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     9361 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     9421 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     9481 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     9541 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9601 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9661 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9721 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9781 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9841 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9901 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9961 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    10021 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    10081 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    10141 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    10201 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    10261 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    10321 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    10381 gcgacacgga aatgttgaat actcat
//
