LOCUS       sequence-469642-       10342 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of Chimera of D-region cCGN in cCGNL1 in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10342)
  AUTHORS   Paschoud S, Guillemot L, Citi S
  TITLE     Distinct domains of paracingulin are involved in its targeting to 
            the actin cytoskeleton and regulation of apical junction assembly.
  JOURNAL   J Biol Chem. 2012 Apr 13;287(16):13159-69. doi: 
            10.1074/jbc.M111.315622. Epub 2012 Feb 7.
  PUBMED    22315225
REFERENCE   2  (bases 1 to 10342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.315622"; journalName: "J Biol Chem"; date: 
            "2012-04-13- 13"; volume: "287"; issue: "16"; pages: "13159-69"
COMMENT     Sequence Label: YFP-cCGNL1-cCGN_B-Myc
FEATURES             Location/Qualifiers
     source          1..10342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(1030..1041)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             5002..5031
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     intron          5077..5649
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(5781..5800)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5846..5901
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5900..5919)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6299..6316)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6470..7058)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6550..6569)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7229..8020,8021..8089))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7852..7871
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8090..8194)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8262..8280
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     polyA_signal    8300..8381
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     8325..8344
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(8498..9523)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8498..9523)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(9532..9861)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9546..9681
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    join(9875..9893,9894..9916,9917..9935,9936..9958,9959..9977,
                     9978..10000,10001..10019,10020..10042,10043..10061,
                     10062..10084,10085..10103,10104..10126,10127..10145)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        10178..10216
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10213..10237
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      join(10337..10342,1..4)
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagct accagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagagc
      721 ggccgcatgg agctgtattt cggcgaatac caacacgtac agcaagaata tggcgtccat
      781 ctgagactcg caagcgatga tagcaaaaag ccaaggaatt cccaacactc taaggcaggc
      841 tcctatggtg tcagtattcg ggtgcaggga atcgatggtc acccctacat agtcctgaat
      901 aacacagagc ggtgtctggc aggcacatct tttcctgaaa atgggccacc atttccatct
      961 ccaatgagaa gtaacctgcc tctgcattcc agcaacgggt ccgtgctgga ggagagcagc
     1021 gcagaggaat tgcagctccc agaaaatccg tatgcccagc ccagcccagt aagaaacctg
     1081 aaacagtcct ctctcttcga aggcaagaac ggggttctag aacgcaaaga ggggccaatg
     1141 aagccatccc acgtgttgaa ctttcagagg catccagagc ttctgcagcc ctatgaccct
     1201 gaaaagaatg aatcgaactt gcaaaaccac cagcctcctg agcataattg gctaaacaca
     1261 ttgacagaag aaggtaccaa caataagaag gcatggacct gcttccccaa gcctggtagt
     1321 tcccagccta ccagccctcc tttggaagat gcgggcaaat ccggcgtgac ggccatccgt
     1381 ttgtgcagct ctgtggtcat cgatgacccc aagaagcaga cctcagtgtg cgtaaatgtt
     1441 gagagctgca ccggggaggc gctggtaggg gaggcccctt cccctagtgg gaggtccccc
     1501 actgcccaca gcccgcacac ccatcctgaa gccaagaaag ccaggccgga cgtgcttccc
     1561 ttccggcgac aagattcagc gggacctgtc ctggacggag ctcggtcccg gaggtcctct
     1621 tcctcgtcgg caacccccac gtcggccaac tctttgtaca gatttctgct tgatgatcag
     1681 gaatgtgcca tccacgctga caatgtgaat cgccacgaaa acagacggta tatccccttc
     1741 ctgccaggaa ctgggcggga tattgacacg ggatcaattc ctggtgtgga tcaactgatt
     1801 gaaaagtttg accaaaaacc cgggcttcag aggagaggaa ggcctgggaa gcggaacaga
     1861 atcaatccag aagacaggaa gaggtccaga agcgtggata gtgcctttcc tttcggtctg
     1921 caggggaact cggagtacct cagcgaattt agccggaacc tgggcaagtc cagcgaacac
     1981 ctcctccgcc cgtcgcaggt gtgcccgcag cggtcacctg cccaggagca ccgcgggaga
     2041 cagagcgtgg gccgcgcctt cgccaagctg cagggggccc acctccggcc tgcccagcag
     2101 agcaaagatg ggaaagctcc agcaaacaga gggggtcggg agagtacaaa cacgcgtgtg
     2161 tcctcccccc cagcccagaa taaaaaggag gacgaaatca agacagccac ggcgacgctg
     2221 atgttacaga atcgggccct agcgacttcg cccgactctg gtgccaagaa gatttctgtg
     2281 aagacattta cttccgcctc caacactcag gccacaccag atctgttgaa aggccaacaa
     2341 gagctcactc aacaaaccaa tgaggagacg gccaagcaga tactttacaa ttacctcaaa
     2401 gaaggaagca ccgataatga tgatgcgact aaacggaaag tcatcaagct agagtcatct
     2461 cggcttgggc tggagcggca gctgcaggag aaggcagaag agtgcagcca actgcaggag
     2521 ctgctggaaa ggagaaaggg cgaggcccag cagagcacca aagagctcca gaacatgaag
     2581 ctccttgtgg accagagtga aagggtacga tgtgggctgg aggcccaggt gaaggagctg
     2641 caggacaagc tgaaacaggc ccaggagcct gagcctgcta aggaggcgct catgaaggac
     2701 ctgttagagg cccgagagct tctggaagag gtcttagagg ggaagcagcg gatggaggag
     2761 cacctgagac tgcgggagcg ggagctgaca gccctgaagg gggccctgaa ggaggaggtg
     2821 gcctcccgtg accaggaggt ggaacatgtc cggcagcagt gccagcgaga tgcagagcag
     2881 cttcgccgga gtatacagga tgcaacccag gaccatgctg cattagaggt ggagaggcag
     2941 aaaatgtcaa ccctggtgcg ggagctgcag aaggagctgg aggagacctc agaggagaca
     3001 gggcattggc agagcatgtt ccagaagaac aaggatgaac ttagagccac taagcaggaa
     3061 ctcctgcagc tgcggatgga gaaggaggag atagaagagg agcttgggga gaagatagag
     3121 gtcttacaaa gggaattagg gcaggcccga gctggtgctg cagatactcg ccaaatggag
     3181 gagcttaaga aggagctgtg ccagacacag aaggaactta aggaactgaa ggaagagcag
     3241 cagaaccagg aggtggctgg gcgtcaccgg gagcgggaat tggagaagca gctaaaggtt
     3301 gaggctgatc gaggccaggg gttggaacag cagaacctcc agctacaaaa aaccctccag
     3361 caactgagac aggactgtga agaggcttcc aaggctcagg tggcagcaga ggcagaggtg
     3421 gcggtgctgg ggcagcgacg ggcagcagtg gaagtgacac ttcgggagac ccaggaggag
     3481 aatgacgagt tccgacggcg tattctgggt ctggaacagc agctaaagga ggcccgaggc
     3541 ctggcagagg gcggggaagt ggcagaggca aggcttcggg acaaggtgca gcggctagag
     3601 gcagagaaac agcggctcga agaggccctg aatgccgctc aggaagagga ggggagcctg
     3661 gcagctgcca agcgggcact ggaggcccgg ctggaggagg cccagcgggg gttggcccgc
     3721 ctagggcagg agcagatcga tagaaaggaa gaaagagaag ccacatcagt cagaaggacc
     3781 ctggagggtg agctggaaga cgtgcagaga aatctgagtc gggccaccca ggagcagaag
     3841 cagttgtctg agaagcttaa agatgagact gagcagaaag agcagttaag gaggctgaag
     3901 aacgagatgg agaacgagcg atggcatctg gacaagacca ttgaaaaatt gcagaaagag
     3961 atggctgaca ttgttgaggt gtcccgtgta tcaacactgg agctccagaa ccagctagat
     4021 gagtacaagg agaaaaatcg cagggaactt gcagaaatgc aaaggcagtt gaaggagaaa
     4081 acgctagagg ctgaaaagtc acgtctgact gccatgaaac tgcaggatga gatgcgtctg
     4141 atggaggagg agttacggga ctaccagaga gctcaggatg aggcgctcac aaaaaggcag
     4201 cttctggagc agacactcaa ggacctggaa tacgagctgg aggccaaaag tcacctcaaa
     4261 gatgaccgaa gcagactcgt caagcagatg gaggacaagg tgtctcagct ggagatggaa
     4321 ctggaagaag aaagaaataa ctcggatttg ctctcggaga ggatcactcg gagcagggaa
     4381 cagatggagc agatgaggag cgagctcctg caggaaagag ctctcaagca ggacttggag
     4441 tgtgacaaga tttccttgga gaggcagaac aaagatttaa agagccggat tatccatctg
     4501 gaaggctcct acaggtccag caaagagggg ctggtggtgc agatggaggc caggatcgcg
     4561 gagctggagg accgtctgga aaacgaggag agggaccggg ccagcctcca gctcagcaac
     4621 cgacggctgg agcggaaggt gaaggagcta gtgatgcaag tggacgatga gcacctgtct
     4681 ctgactgatc agaaggacca gcttagcttg cgcttgaaag caatgaaacg acaagtagag
     4741 gaggccgagg aggagatcga cagactagaa agttctaaaa agaagctgca gagggagctg
     4801 gaggagcaga tggatgtgaa tgagcagctg cagggacaac tcaattccat gaagaaggac
     4861 ctaagactca agaagctgcc gagcaaagtg ctggacgaca cggatgatga tgacctcagt
     4921 acggacgggg ggagcctcca cgaggcgccg ctgagctaca ccttccccaa ggacagcgct
     4981 gccaccagcc agatcgtcga tgaacaaaaa ctcatctcag aagaggatct gaattaggtc
     5041 gacctcgacg atatctctag agctgagaac ttcagggtga gtttggggac ccttgattgt
     5101 tctttctttt tcgctattgt aaaattcatg ttatatggag ggggcaaagt tttcagggtg
     5161 ttgtttagaa tgggaagatg tcccttgtat caccatggac cctcatgata attttgtttc
     5221 tttcactttc tactctgttg acaaccattg tctcctctta ttttcttttc attttctgta
     5281 actttttcgt taaactttag cttgcatttg taacgaattt ttaaattcac ttttgtttat
     5341 ttgtcagatt gtaagtactt tctctaatca cttttttttc aaggcaatca gggtatatta
     5401 tattgtactt cagcacagtt ttagagaaca attgttataa ttaaatgata aggtagaata
     5461 tttctgcata taaattctgg ctggcgtgga aatattctta ttggtagaaa caactacacc
     5521 ctggtcatca tcctgccttt ctctttatgg ttacaatgat atacactgtt tgagatgagg
     5581 ataaaatact ctgagtccaa accgggcccc tctgctaacc atgttcatgc cttcttctct
     5641 ttcctacagc tcctgggcaa cgtgctggtt gttgtgctgt ctcatcattt tggcaaagaa
     5701 ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg
     5761 gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag
     5821 ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt
     5881 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat
     5941 cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac
     6001 aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta
     6061 ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt
     6121 ttttctttaa catccctaaa attttcctta catgttttac tagccagatt tttcctcctc
     6181 tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct cgactgcatt
     6241 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     6301 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6361 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6421 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6481 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6541 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6601 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6661 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6721 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6781 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6841 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6901 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6961 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     7021 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7081 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     7141 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     7201 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7261 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7321 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7381 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7441 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7501 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     7561 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7621 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7681 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     7741 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7801 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
     7861 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     7921 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     7981 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     8041 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     8101 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     8161 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     8221 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     8281 cctttcgtct tcactcgagg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     8341 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     8401 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
     8461 acaaatgtgg tatggctgat tatgatcctc tagaactcta ttcctttgcc ctcggacgag
     8521 tgctggggcg tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg
     8581 ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg
     8641 cgtcgcatcg accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat
     8701 agagttggtc aagaccaatg cggagcatat acgcccggag ccgcggcgat cctgcaagct
     8761 ccggatgcct ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc
     8821 agaagaagat gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa
     8881 tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca
     8941 cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg
     9001 cgacggacgc actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag
     9061 caatcgcgca tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg
     9121 ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat cgcatccatg gcctccgcga
     9181 ccggctgcag aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg
     9241 cacggcggga gatgcaatag gtcaggctct cgctgaattc cccaatgtca agcacttccg
     9301 gaatcgggag cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat
     9361 cggcgcagct atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac
     9421 gagattcttc gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca
     9481 gaaacttctc gacagacgtc gcggtgagtt caggcttttt catggaagct ttttgcaaaa
     9541 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc
     9601 ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact
     9661 gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt
     9721 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt
     9781 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     9841 tccacaccct aactgacaca ctcgagttta ccactcccta tcagtgatag agaaaagtga
     9901 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
     9961 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
    10021 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
    10081 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
    10141 agagaaaagt gaaagtcgag ctcggtaccc gggtcgaggt aggcgtgtac ggtgggaggc
    10201 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
    10261 ttttgacctc catagaagac accgggaccg atccagcctc cgcggccccg aattcgagct
    10321 cggtacccgg ggatccgcca cc
//
