LOCUS       sequence-469643-        9976 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of Chimera of B-region cCGNL1 in cCGN in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9976)
  AUTHORS   Paschoud S, Guillemot L, Citi S
  TITLE     Distinct domains of paracingulin are involved in its targeting to 
            the actin cytoskeleton and regulation of apical junction assembly.
  JOURNAL   J Biol Chem. 2012 Apr 13;287(16):13159-69. doi: 
            10.1074/jbc.M111.315622. Epub 2012 Feb 7.
  PUBMED    22315225
REFERENCE   2  (bases 1 to 9976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.315622"; journalName: "J Biol Chem"; date: 
            "2012-04-13- 13"; volume: "287"; issue: "16"; pages: "13159-69"
COMMENT     Sequence Label: GFP-cCGN-cCGNL1_B-Myc
FEATURES             Location/Qualifiers
     source          1..9976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4636..4665
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     intron          4711..5283
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(5415..5434)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5480..5535
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5534..5553)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5933..5950)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6104..6692)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6184..6203)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6863..7654,7655..7723))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7486..7505
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7724..7828)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7896..7914
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     polyA_signal    7934..8015
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     7959..7978
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(8132..9157)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8132..9157)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(9166..9495)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9180..9315
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    join(9509..9527,9528..9550,9551..9569,9570..9592,9593..9611,
                     9612..9634,9635..9653,9654..9676,9677..9695,9696..9718,
                     9719..9737,9738..9760,9761..9779)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        9812..9850
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9847..9871
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      join(9971..9976,1..4)
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagagc
      721 ggccgcatgg ctgagccccg gggccctgta gaccatggag tccagattcg cttcatcaca
      781 gagccaatgg gtaatgcaga gatggacacc ctgcgtcgtg gtggacgacg tcctgctaag
      841 gatgctagag ccaataccta tggagtcgct gtgcgtgtgc agggcattgc tgggcagccc
      901 tttgtggtgc tcaacagtgg ggagcaaggt agtgactcct ttggggtcca gatcaagggg
      961 accaacaaca gagggcctcc aggagctcta agctctgact ctgaactccc tgaaagcacc
     1021 tactctcatg ccaaggaatt tcctgcccgc tcacagggca gcatgtctga tgaggagctt
     1081 ggggcccatt ggaatggaag gctactccga tcccagtccc aggcatcact gaaaggccct
     1141 gcccctgtga gtccgagcac caggagcact agcctgctgc agctggcccc tgaagaattc
     1201 tcccctagtg ggaggtcccc cactgcccac agcccgcaca cccatcctga agccaagaaa
     1261 gccaggccgg acgtgcttcc cttccggcga caagattcag cgggacctgt cctggacgga
     1321 gctcggtccc ggaggtcctc ttcctcgtcg gcaaccccca cgtcggccaa ctctttgtac
     1381 agatttctgc ttgatgatca ggaatgtgcc atccacgctg acaatgtgaa tcgccacgaa
     1441 aacagacggt atatcccctt cctgccagga actgggcggg atattgacac gggatcaatt
     1501 cctggtgtgg atcaactgat tgaaaagttt gaccaaaaac ccgggcttca gaggagagga
     1561 aggcctggga agcggaacag aatcaatcca gaagacagga agaggtccag aagcgtggat
     1621 agtgcctttc ctttcggtct gcaggggaac tcggagtacc tcagcgaatt tagccggaac
     1681 ctgggcaagt ccagcgaaca cctcctccgc gatgggcatc agttccctac ccattggact
     1741 cctagcacaa agcgtgacag ccacatgggc aactccaaac agtcatccca gaatcagggc
     1801 cctcttggtg gatttagctg ttcccgtcag acccaggact gggttcttca gagttttgag
     1861 gagccacggg ggagagcctg ggaccctggc atgctacagt tcaaatcaac tccagacctt
     1921 ctccgagacc agcaggagac ggccccaccg ggcagtgtgg accatgtgaa ggccactatc
     1981 tatagcatcc tgagggaggg aagctcagaa accgaaacct ctgtgaggag gaaggtcaat
     2041 ttggtgctgg aacagatgca gcctagcggc cgcctggtga tgacttctgg atctgctaag
     2101 ggcctgactg ggcagagtga gctcagccaa aaagtggagg agctacagca gaaactggat
     2161 gaggaggtga agaaacggcc gaagctagag tcatctcggc ttgggctgga gcggcagctg
     2221 caggagaagg cagaagagtg cagccaactg caggagctgc tggaaaggag aaagggcgag
     2281 gcccagcaga gcaccaaaga gctccagaac atgaagctcc ttgtggacca gagtgaaagg
     2341 gtacgatgtg ggctggaggc ccaggtgaag gagctgcagg acaagctgaa acaggcccag
     2401 gagcctgagc ctgctaagga ggcgctcatg aaggacctgt tagaggcccg agagcttctg
     2461 gaagaggtct tagaggggaa gcagcggatg gaggagcacc tgagactgcg ggagcgggag
     2521 ctgacagccc tgaagggggc cctgaaggag gaggtggcct cccgtgacca ggaggtggaa
     2581 catgtccggc agcagtgcca gcgagatgca gagcagcttc gccggagtat acaggatgca
     2641 acccaggacc atgctgcatt agaggtggag aggcagaaaa tgtcaaccct ggtgcgggag
     2701 ctgcagaagg agctggagga gacctcagag gagacagggc attggcagag catgttccag
     2761 aagaacaagg atgaacttag agccactaag caggaactcc tgcagctgcg gatggagaag
     2821 gaggagatag aagaggagct tggggagaag atagaggtct tacaaaggga attagggcag
     2881 gcccgagctg gtgctgcaga tactcgccaa atggaggagc ttaagaagga gctgtgccag
     2941 acacagaagg aacttaagga actgaaggaa gagcagcaga accaggaggt ggctgggcgt
     3001 caccgggagc gggaattgga gaagcagcta aaggttgagg ctgatcgagg ccaggggttg
     3061 gaacagcaga acctccagct acaaaaaacc ctccagcaac tgagacagga ctgtgaagag
     3121 gcttccaagg ctcaggtggc agcagaggca gaggtggcgg tgctggggca gcgacgggca
     3181 gcagtggaag tgacacttcg ggagacccag gaggagaatg acgagttccg acggcgtatt
     3241 ctgggtctgg aacagcagct aaaggaggcc cgaggcctgg cagagggcgg ggaagtggca
     3301 gaggcaaggc ttcgggacaa ggtgcagcgg ctagaggcag agaaacagcg gctcgaagag
     3361 gccctgaatg ccgctcagga agaggagggg agcctggcag ctgccaagcg ggcactggag
     3421 gcccggctgg aggaggccca gcgggggttg gcccgcctag ggcaggagca gctagcactg
     3481 aaccgggccc tagaggagga ggggaagcag cgggaggcgc tccggcggag caaggctgag
     3541 ctggaggaac agaagcgctt gctggacaag actgtgtgcc agctcaacaa ggagctggag
     3601 cagattggga acgattctaa gcaggctctg cagcagctcc aggcccagct ggaggattac
     3661 aaggagaagg cccggcggga ggtggcggat gcccagcgtc aggccaagga atgggccact
     3721 gaggctgaga agaactcagg ggggctgagc cggcttcagg atgagaccca gaggctccgg
     3781 caggccctgc aggcatccca ggctgaccgg gacacagcaa ggctggataa agagctgctg
     3841 gcccagcgac tgcagggcct ggagcaagag gcagagaaca agaagcgctc ccaggatgac
     3901 agggcccggc agctgaaggg tcttgaggaa aaagtctcac ggctggaagc agagttagat
     3961 gaggagagga gtaccgtgga gctgctgaca gaacgtgtga ctcgtggccg ggaccaggta
     4021 gaccagctga ggtcagagct catgcaggag agatctgctc gacaggacct ggagtgtgac
     4081 aaaatctccc tagagagaca gaacaaggac ttgaagagcc gattggccag ctcagaaggc
     4141 ttccagaagc ccagtgccag cctctctcag cttgaatccc agaatcagga gttgcaggag
     4201 cggctgcagg ctgaagagag ggagaagacg gtccttcagt ccaccaaccg aaaactggag
     4261 cggagggtta aagagctgtc catccaaatt gatgatgagc ggcagcatgt caatgaccag
     4321 aaggaccagc taagcctgag ggtgaaggct ttgaagcggc aggtggatga agcagaagag
     4381 gagattgagc gactggatgg cctgaggaag aaggcccagc gtgagctgga agagcaacat
     4441 gaggtcaatg aacagctcca ggcccggatc aaaaccctag agaaggactc ctggcgcaaa
     4501 gcctcccgtt cagctgctga gtcagcccag cgtgaagggc tgagctcgga tgaggagttt
     4561 gacagtgtct acgacccttc ttccattgca tcgctgctta cggagagcaa cctgcagacc
     4621 agctcctgta tcgatgaaca aaaactcatc tcagaagagg atctgaatta ggtcgacctc
     4681 gacgatatct ctagagctga gaacttcagg gtgagtttgg ggacccttga ttgttctttc
     4741 tttttcgcta ttgtaaaatt catgttatat ggagggggca aagttttcag ggtgttgttt
     4801 agaatgggaa gatgtccctt gtatcaccat ggaccctcat gataattttg tttctttcac
     4861 tttctactct gttgacaacc attgtctcct cttattttct tttcattttc tgtaactttt
     4921 tcgttaaact ttagcttgca tttgtaacga atttttaaat tcacttttgt ttatttgtca
     4981 gattgtaagt actttctcta atcacttttt tttcaaggca atcagggtat attatattgt
     5041 acttcagcac agttttagag aacaattgtt ataattaaat gataaggtag aatatttctg
     5101 catataaatt ctggctggcg tggaaatatt cttattggta gaaacaacta caccctggtc
     5161 atcatcctgc ctttctcttt atggttacaa tgatatacac tgtttgagat gaggataaaa
     5221 tactctgagt ccaaaccggg cccctctgct aaccatgttc atgccttctt ctctttccta
     5281 cagctcctgg gcaacgtgct ggttgttgtg ctgtctcatc attttggcaa agaattcact
     5341 cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac
     5401 aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt
     5461 gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat
     5521 tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat
     5581 gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt
     5641 tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat
     5701 agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct
     5761 ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga
     5821 ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgactg cattaatgaa
     5881 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     5941 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     6001 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     6061 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6121 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6181 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     6241 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     6301 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     6361 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     6421 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     6481 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     6541 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     6601 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     6661 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     6721 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     6781 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     6841 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     6901 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     6961 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     7021 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7081 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     7141 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     7201 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     7261 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     7321 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     7381 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     7441 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac
     7501 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     7561 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     7621 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     7681 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     7741 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     7801 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     7861 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     7921 gtcttcactc gaggcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     7981 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     8041 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
     8101 gtggtatggc tgattatgat cctctagaac tctattcctt tgccctcgga cgagtgctgg
     8161 ggcgtcggtt tccactatcg gcgagtactt ctacacagcc atcggtccag acggccgcgc
     8221 ttctgcgggc gatttgtgta cgcccgacag tcccggctcc ggatcggacg attgcgtcgc
     8281 atcgaccctg cgcccaagct gcatcatcga aattgccgtc aaccaagctc tgatagagtt
     8341 ggtcaagacc aatgcggagc atatacgccc ggagccgcgg cgatcctgca agctccggat
     8401 gcctccgctc gaagtagcgc gtctgctgct ccatacaagc caaccacggc ctccagaaga
     8461 agatgttggc gacctcgtat tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg
     8521 ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt
     8581 gccggacttc ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg
     8641 acgcactgac ggtgtcgtcc atcacagttt gccagtgata cacatgggga tcagcaatcg
     8701 cgcatatgaa atcacgccat gtagtgtatt gaccgattcc ttgcggtccg aatgggccga
     8761 acccgctcgt ctggctaaga tcggccgcag cgatcgcatc catggcctcc gcgaccggct
     8821 gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc
     8881 gggagatgca ataggtcagg ctctcgctga attccccaat gtcaagcact tccggaatcg
     8941 ggagcgcggc cgatgcaaag tgccgataaa cataacgatc tttgtagaaa ccatcggcgc
     9001 agctatttac ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa gcacgagatt
     9061 cttcgccctc cgagagctgc atcaggtcgg agacgctgtc gaacttttcg atcagaaact
     9121 tctcgacaga cgtcgcggtg agttcaggct ttttcatgga agctttttgc aaaagcctag
     9181 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc
     9241 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg
     9301 agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg
     9361 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga
     9421 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     9481 ccctaactga cacactcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     9541 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     9601 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
     9661 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
     9721 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
     9781 aagtgaaagt cgagctcggt acccgggtcg aggtaggcgt gtacggtggg aggcctatat
     9841 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     9901 cctccataga agacaccggg accgatccag cctccgcggc cccgaattcg agctcggtac
     9961 ccggggatcc gccacc
//
